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pHH0103_CACNB2-1/2

Plasmid

#91314

Purpose

Protein expression and purification of human SH3 domain construct CACNB2-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CACNB2-1/2 (CACNB2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_EPS8L1-1/1-IS2/2

Plasmid

#91315

Purpose

Protein expression and purification of human SH3 domain construct EPS8L1-1/1-IS2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

EPS8L1-1/1-IS2/2 (EPS8L1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_FCHSD1-2/2

Plasmid

#91258

Purpose

Protein expression and purification of human SH3 domain construct FCHSD1-2/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

FCHSD1-2/2 (FCHSD1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYO1F-1/1

Plasmid

#91260

Purpose

Protein expression and purification of human SH3 domain construct MYO1F-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MYO1F-1/1 (MYO1F Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DLG4-1/1

Plasmid

#91266

Purpose

Protein expression and purification of human SH3 domain construct DLG4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DLG4-1/1 (DLG4 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pRSM4

Plasmid

#101642

Purpose

Encodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

KCyG4219

Tags

6XHis

Expression

Bacterial

Mutation

C-terminal residues (221-223) deletion. Ser220Val…

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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HeFm1SpCas9

Plasmid

#92110

Purpose

Expression plasmid for human codon-optimized increased fidelity HeFm1SpCas9 (without U6-sgRNA coding sequence)

Depositor

Ervin Welker

Article

Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

Insert

3xFLAG-NLS-Streptococcus pyogenes Highly enhanced Fidelity mut1 Cas9-NLS

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A, Q695A, K848A, Q926A, K1003A, R1060A

Promoter

Cbh

Available Since

Oct. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_CASKIN2-1/1

Plasmid

#91261

Purpose

Protein expression and purification of human SH3 domain construct CASKIN2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

CASKIN2-1/1 (CASKIN2 Human)

Expression

Bacterial

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 N184X

Plasmid

#92373

Purpose

Inducible expression of M87 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, N184X

Promoter

Tet-responsive P tight

Available Since

June 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP S245X with 5'UTR

Plasmid

#92376

Purpose

Inducible expression of S245X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M87 S245X

Plasmid

#92374

Purpose

Inducible expression of M87 S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 S245X

Plasmid

#92372

Purpose

Inducible expression of M1S245X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, S245X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTRE/SP M1 N184X

Plasmid

#92371

Purpose

Inducible expression of M1 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, N184X

Promoter

Tet-responsive P tight

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/SP S245X with 5'UTR

Plasmid

#92370

Purpose

expression of 245X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

S245X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M87 N184X

Plasmid

#92367

Purpose

expression of M87 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, N184X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1 N184X

Plasmid

#92365

Purpose

expression of M1 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, N184X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 3xFLAG TPP1 88-249 delta166-172

Plasmid

#53550

Purpose

mammalian expression of TPP1 OB-fold domain with 166-172 replaced with "GSSG"

Depositor

Kathleen Collins

Article

Sexton et al J Biol Chem. 2012 Oct 5;287(41):34455-64

Insert

TPP1 (ACD Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

contains aa 88-249, with aa 166-172 replaced with…

Promoter

CMV

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pFSW Doc2beta 6X mutant

Plasmid

#128816

Purpose

To make virus expressing Doc2beta 6X mutant

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

IRES-EGFP

Mutation

D163A, D218A, D220N, D303A, D357A, D359A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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SNX9-PX (246-376)

Plasmid

#119090

Purpose

Bacterial expression of human phox homology (PX) domain, SNX9-PX (246-376)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX9-PX (246-376) (SNX9 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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PI3KC2gamma-PX (1200-1310)

Plasmid

#119121

Purpose

Bacterial expression of human phox homology (PX) domain, PI3KC2gamma-PX (1200-1310)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

PI3KC2gamma-PX (1200-1310) (PIK3C2G Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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