We narrowed to 28,330 results for: STI

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pCRISPRa_all-in-one_Bip gRNA1

Plasmid

#183696

Purpose

Expresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selective

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

gRNA targeting chinese hamster Bip (Hspa5)

Use

CRISPR

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pSBtet-Bla-dCas9-KRAB-MeCP2_hU6-A10

Plasmid

#196081

Purpose

Inducible CRISPRi vector conferring blasticidin S resistance, expressing gRNA targeting mouse Adam10.

Depositor

Joanna Bereta

Article

Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

Insert

gRNA targeting Adam10

Use

CRISPR

Expression

Mammalian

Mutation

Cloned using SapI sites - destroyed after inserti…

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pShuttle.Cre

Plasmid

#192930

Purpose

Gateway compatible vector with BsaI cloning site and Cre expression

Depositor

Thorsten Stiewe

Article

Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

Type

Empty backbone

Use

Gateway-compatible cloning vector

Promoter

CMV

Available Since

Dec. 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR4 Ta-SRO1 JN177

Plasmid

#192546

Purpose

pENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)

Use

Gateway Cloning

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR4 Ta-sro1 SR3

Plasmid

#192547

Purpose

pENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codon

Depositor

Lennart Wirthmueller

Article

Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

Insert

SIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)

Use

Gateway Cloning

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pENT-L1-Kozak-Ark-L2

Plasmid

#186362

Purpose

Entry clone with ORF encoding Ciona robusta Aurora kinase A flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Insert

Ciona robusta Aurora kinase A

Use

Expression of a fluorescent centrosome marker

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMA4000

Plasmid

#184857

Purpose

Cre-excisable TFAM in Blasticidin-resistannce retroviral vector

Depositor

Mikhail Alexeyev

Article

Kozhukhar et al Cells. 2022 Jul 11;11(14). pii: cells111

Insert

TFAM (TFAM Human)

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Aug. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a(+)_co_L-HicDH

Plasmid

#185453

Purpose

CDS of L-HicDH codon optimized for expression in Synechocystis sp. PCC 6803 in pET21a(+).

Depositor

Wolfgang Kroutil

Article

Jurkas et al Eng Microbiol. 2021 Nov 26;2(1):100008.

Insert

L-HicDH from Lactobacillus confusus DSM 20196

Expression

Bacterial

Promoter

T7

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pK-eCFP

Plasmid

#176559

Purpose

Expression of eCFP for auxotrophic selection in the absence of histidine

Depositor

Markus Ralser

Article

Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

Insert

eCFP

Expression

Yeast

Promoter

TDH1(GAP)

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pN1mcherry-NKD2EFMUT

Plasmid

#174333

Purpose

encodes human NKD2 132DFD134>AFA mutant (two D mutated to A)cDNA with a C-terminal mCherry fusion.

Depositor

Sonal Srikanth

Article

Wu et al Cell Rep. 2021 Aug 24;36(8):109603. doi:

Insert

Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2 Human)

Tags

mCherry tag

Expression

Mammalian

Mutation

NKD2132DFD134>AFA (substitutions of D132 and D…

Promoter

CMV

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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p1A CbbL-

Plasmid

#162693

Purpose

p1A negative control expressing inactive CbbLS (carbamylated K194M mutation) and Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

H. neapolitanus CbbLS and S. elongatus Prk

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

CbbL K194M (inactive rubisco)

Promoter

PLtet0-1 promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pProEx-Htb-cytATL2(R323Q)-6Gly-sfGFP

Plasmid

#124066

Purpose

E. coli. exp. and purif. of X. laevis cytATL-R232Q fragment fused to GFP in c-terminus.

Depositor

Tom Rapoport

Article

Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

Insert

cytATL-R232Q-sfGFP

Tags

sfGFP

Expression

Bacterial

Mutation

R232Q

Promoter

trc

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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zebrafish SLC2A2 (zfSLC2A2)

Plasmid

#65103

Purpose

Full length SLC2A2 cDNA (Danio rerio)

Depositor

Josep Planas

Article

Castillo et al Am J Physiol Regul Integr Comp Physiol.

Insert

SLC2A2 (slc2a2 Zebrafish)

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only
hC73S-HA

Plasmid

#111529

Purpose

Expresses human KCNB1 C73S in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human C73S-Kv2.1 + HA (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

C73S

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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hC73A-HA

Plasmid

#111526

Purpose

Expresses human KCNB1 C73A in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al J Neurosci. 2012 Mar 21;32(12):4133-44.

Insert

human C73A-Kv2.1 + HA (KCNB1 Human)

Tags

HA

Expression

Mammalian

Mutation

C73A

Promoter

CMV

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM W4108A

Plasmid

#83462

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (502)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, W4107A

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM S4144D

Plasmid

#83460

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a point mutant that disrupts the second calmodulin binding domain (513)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, S4144D

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO CD16.7-h-PKD1 FLM (4078-4169)

Plasmid

#83463

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and PKD1 sequence that corresponds to 4078 to 4169 of PC1 (96)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4169

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS S4144D-Myc/His

Plasmid

#83459

Purpose

Human PC1-CTp30 expression cassette with a point mutant that disrupts the second calmodulin binding domain (514)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4107-4303, S4144D

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO h-PKD1 FLS (4127-4303)-Myc/His

Plasmid

#83457

Purpose

Human PC1-CTp30 expression cassette lacking a Calmodulin binding domain (delta C1) (632)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

Amino-acids 4127-4303

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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