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Bov.hsc70.RSET.FL.wt(NarI)

Plasmid

#12532

Depositor

David McKay

Article

Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

Insert

bovine 70 kDa heat shock cognate protein full length wild type

Expression

Bacterial

Mutation

wild type

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pJL1 eforRed

Plasmid

#106320

Purpose

Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter

Depositor

James Collins

Article

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

eforRed

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pBAD-KIRIN1-GR

Plasmid

#107417

Purpose

Bacterial Expression plasmid for potassium ion ratiometric indicator KIRIN1-GR

Depositor

Robert Campbell

Article

Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

Insert

KIRIN1-GR

Expression

Bacterial

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pTAL6-BB

Plasmid

#36034

Depositor

Tom Ellis

Article

Blount et al PLoS One. 2012;7(3):e33279. Epub 2012 Ma

Type

Empty backbone

Expression

Yeast

Promoter

PTEF1

Available Since

July 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCap-101-Apr

Plasmid

#227507

Purpose

Capture plasmid with apramycin resistance and pIJ101 replicon

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Type

Empty backbone

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EF1a-FLEx-ASAP4e-Kv-WPRE

Plasmid

#201033

Purpose

Expresses somatically enriched ASAP4e from the EF-1alpha promoter in a cre-dependent manner

Depositor

Michael Lin

Article

Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

Insert

ASAP4b-Kv

Use

AAV

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-MCS

Plasmid

#185594

Purpose

Control lentivector that can be used to express inserts from the PGK promoter and/or shRNA cassettes from U6.

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Orange_CaMBI_110

Plasmid

#124094

Purpose

orange calcium-modulated bioluminescent indicator, 110nM

Depositor

Michael Lin

Article

Oh et al Nat Chem Biol. 2019 Apr 1. pii: 10.1038/

Insert

Orange CaMBI 110

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMK90

Plasmid

#13323

Depositor

Martin Gellert

Article

Mizuuchi et al J Biol Chem. 1984 Jul 25. 259(14):9199-2

Insert

gyrA

Expression

Bacterial

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pG2J

Plasmid

#102882

Purpose

Expresses OsTIR1 via the ADH1 promoter, and mEGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

Depositor

Matthias Heinemann

Article

Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

Inserts

OsTIR1
mEGFP-AID

Tags

3xMyc and truncated AID (71-114)

Expression

Yeast

Promoter

ADH1 and TEF1

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTET2 (CD)

Plasmid

#89736

Purpose

Expresses the CD-domain truncation to mouse TET2 in mammalian cells

Depositor

Yue Xiong

Article

Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

Insert

TET2 (Tet2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

CD (cysteine-rich, dioxygenase domain) truncation…

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-mCherry

Plasmid

#65259

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA6.MCV.LT339.V5.4

Plasmid

#28193

Depositor

Patrick Moore

Article

Shuda et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

Insert

MCPyV Truncated Tumor Large T antigen (399 tumor strain)

Tags

V5

Expression

Bacterial and Mammalian

Available Since

Oct. 3, 2011

Availability

Academic Institutions and Nonprofits only

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qMaLioffG

Plasmid

#212825

Purpose

green fluorescent FLIM indicator for endogenous ATP imaging

Depositor

Tetsuya Kitaguchi

Article

Arai et al Nat Commun. 2025 Nov 13;16(1):9972. doi:

Insert

qMaLioffG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pME-tdTomato no stop

Plasmid

#82404

Purpose

Gateway-compatible tdTomato fusion reporter

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

tdTomato (lacks stop codon)

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pTKDP-dhfr

Plasmid

#71323

Purpose

Donor plasmid - trimethoprim resistant donor cassette

Depositor

Thomas Kuhlman

Article

Tas et al PLoS One. 2015 Sep 2;10(9):e0136963. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pIC111

Plasmid

#44435

Depositor

Iain Cheeseman, Arshad Desai

Article

Cheeseman et al Sci STKE. 2005 Jan 11;2005(266):pl1.

Type

Empty backbone

Use

Retroviral

Tags

EGFP, His, and PreScission cleavage site (2x)

Expression

Mammalian

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

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K560GFP

Plasmid

#129761

Purpose

Expresses Drosophila Kinesin-1 truncated after coil 1 at 560 and tagged with GFP for single-molecule motor assays

Depositor

William Hancock

Insert

KHC (Khc Fly)

Tags

GFP and His6

Expression

Bacterial

Mutation

Truncation at 559

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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GNAS_pLX307

Plasmid

#98339

Purpose

Lentiviral expression of GNAS

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

GNAS (GNAS Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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EZH2 E10.3 gRNA

Plasmid

#90685

Purpose

3rd generation lentiviral gRNA plasmid targeting human EZH2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

EZH2 (Guide Designation E10.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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