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AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA (AAV1)

Viral prep

#65453-AAV1

Purpose

Ready-to-use AAV1 particles produced from AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA (#65453). In addition to the viral particles, you will also receive purified AAV pTRE-FSF-FLEX-EGFP-WPRE-bGHpA plasmid DNA. TRE-driven, Cre and Flp-dependent expression of EGFP. These AAV preparations are suitable purity for injection into animals.

Depositor

Hongkui Zeng

Publication

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Promoter

tetO

Tags

EGFP (Cre and Flp-dependent)

Available since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only
pOPINK-ALG13 (OTU, aa 216-353)

Plasmid

#61414

Purpose

Expresses human ALG13 (OTU domain) in E. coli.

Depositor

David Komander

Publication

Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

Insert

ALG13 (Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13) (ALG13 Human)

Tags

His6-GST-3C

Expression

Bacterial

Mutation

Deleted aa 1-215 and aa 354-1137.

Promoter

T7

Available since

March 12, 2015

Availability

Academic Institutions and Nonprofits only

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SM272_EF1A-dHyperLbCas12a-VPR-T2A-EGFP

Plasmid

#244210

Purpose

Mammalian expression of dHyperLbCas12a-VPR with EGFP selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a-VPR

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SM468_EF1A-dHyperLbCas12a-T2A-EGFP

Plasmid

#244220

Purpose

Mammalian expression of dHyperLbCas12a with EGFP selectable marker

Depositor

Tim Reddy

Publication

Melore et al Nat Commun. 2026 Feb 12. doi: 10.1038/s4

Insert

dHyperLbCas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pKEW302-AcrVA4

Plasmid

#115656

Purpose

Expresses AcrVA4 in bacteria, under TetR control.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA4

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAc5.1A-Dm-miR-13a

Plasmid

#22761

Depositor

Elisa Izaurralde

Publication

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 13a (mir-13a Fly, primary transcript)

Use

Drosophila expression

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-2a-1

Plasmid

#22764

Depositor

Elisa Izaurralde

Publication

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 2a-1 (mir-2a-1 Fly, primary transcript)

Use

Drosophila expression

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAc5.1A-Dm-miR-92a

Plasmid

#22767

Depositor

Elisa Izaurralde

Publication

Rehwinkel et al Mol Cell Biol. 2006 Apr . 26(8):2965-75.

Insert

micro RNA 92a (mir-92a Fly, primary transcript)

Use

Drosophila expression

Available since

Jan. 30, 2013

Availability

Academic Institutions and Nonprofits only

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sgRNA AAVS1

Plasmid

#128119

Purpose

Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Publication

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA AAVS1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTW195b63

Plasmid

#136931

Purpose

Directed evolution of SpCas11

Depositor

David Liu

Publication

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII(N)-npuN

Use

CRISPR

Expression

Bacterial

Mutation

none

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-Rpp38-C1

Plasmid

#134548

Purpose

Expresses YFP-Rpp38 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rpp38 (RPP38 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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YFP-RPL23a-C1

Plasmid

#134535

Purpose

Expresses YFP-RPL23a fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RPL23a (RPL23A Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YFP-Rpp21-C1

Plasmid

#134542

Purpose

Expresses YFP-Rpp21 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rpp21 (RPP21 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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YFP-Rpp29-C1

Plasmid

#134545

Purpose

Expresses YFP-Rpp29 fusion protein

Depositor

Susan Janicki

Publication

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Rpp29 (POP4 Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-FullLength-TET1-LLAA(20)

Plasmid

#124397

Purpose

Full-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cells

Depositor

Shaun Cowley

Publication

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

FLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L897A/L900A mutations (full length protein is 213…

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP Δgag/pol

Plasmid

#101343

Purpose

Deletion from the start of gag until 229 bases before the cPPT

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Mutation

delta GagPol

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSMF2

Plasmid

#112819

Purpose

Expresses wild-type Rpb1 in yeast cells

Depositor

Stephen Fuchs

Publication

Morrill et al J Biol Chem. 2016 May 27;291(22):11540-5

Insert

RPB1 (RPO21 Budding Yeast)

Expression

Yeast

Promoter

Native promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCW57.1-MBD3L2

Plasmid

#106332

Purpose

All-in-one lentivector with tet-inducible MBD3L2

Depositor

Stephen Tapscott

Publication

Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

Insert

methyl-CpG binding domain protein 3 like 2 (MBD3L2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

tight TRE promoter

Available since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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ABCA1 promoter luciferase reporter (P2 fragment)

Plasmid

#86444

Purpose

Human ABCA1 promoter luciferase reporter (P2 fragment)

Depositor

David Mu

Publication

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Human ABCA1 (ABCA1 Human)

Use

Luciferase

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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ABCA1 promoter luciferase reporter (P3 fragment)

Plasmid

#86445

Purpose

Human ABCA1 promoter luciferase reporter (P3 fragment)

Depositor

David Mu

Publication

Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s413

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Human ABCA1 (ABCA1 Human)

Use

Luciferase

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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