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pCY 3040-04

Plasmid

#36219

Depositor

Anne Robinson

Publication

Young et al Yeast. 2012 Mar;29(3-4):119-36. doi: 10.

Insert

TRP1

Use

Yeast cassette for pcr and homologous recombinati…

Tags

yEpolylinker-yEGFP-TRP1

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Zeo/pTER shMDC1 (764-1)

Plasmid

#22194

Depositor

Eric Campeau

Insert

shMediator of DNA damage checkpoint 1

Use

Lentiviral and RNAi

Available since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST P38KTRDronpa

Plasmid

#205763

Purpose

Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Publication

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

P38 KTR Dronpa

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCMV Puro DEST ERKKTRrsFastLime

Plasmid

#205765

Purpose

Expression of ERK KTR rsFastLime under CMV promoter (With Puromycin Resistance)

Depositor

Edward Boyden

Publication

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

ERK KTR rsFastLime

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pFast-Flag-hHus1

Plasmid

#222470

Purpose

Expresses hHus1 in insect cells

Depositor

Aziz Sancar

Publication

Lindsey-Boltz et al Proc Natl Acad Sci U S A. 2001 Sep 25;98

Insert

hHus1 (HUS1 Human)

Tags

Flag epitope

Expression

Insect

Available since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-CAGGS (JDW 912)

Plasmid

#224472

Purpose

Gateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

CAGGS Promoter

Expression

Mammalian

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBES1-LacOx50-blast

Plasmid

#210221

Purpose

Integrates 50xLacO repeats upstream of the BES1 promoter with a blasticidin gene downstream of the promoter.

Depositor

Calvin Tiengwe

Publication

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pXJ40-Flag-iBox-PAK4cat

Plasmid

#190178

Purpose

expresses Flag-tagged iBox-PAK4cat

Depositor

Bianxiao Cui

Publication

Li et al Nano Lett. 2019 Oct 9;19(10):6955-6963.

Insert

iBox-PAK4cat

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLBS.EF/mScarlet(i)-hCdt100Nt

Plasmid

#129334

Purpose

Cell cycle reporter, lentivirus

Depositor

Yasushi Okada

Publication

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

mScarlet(i) (Addgene 85044)

Use

Lentiviral

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-TorT(sp)

Plasmid

#111255

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter followed by the E. coli TorT signal peptide

Depositor

Rosemarie Wilton

Publication

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

TorT signal peptide

Expression

Bacterial

Promoter

Pc

Available since

July 12, 2018

Availability

Academic Institutions and Nonprofits only

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NovoSplit657(B)-mNeonGreen

Plasmid

#255885

Purpose

Expression plasmid for NovoSplit657(B), the second part of the NovoSplit system, fused to mNeonGreen for fluorescence labeling.

Depositor

Julia Mahamid

Publication

Tran et al Science. 2026 Jul 16:eaeb0822. doi: 10.1

Insert

NovoSplit657(B)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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LgBiT-dSaCas9

Plasmid

#236758

Purpose

Split NanoLuc luciferase biosensor fused to dSaCas9

Depositor

David Segal

Publication

Heath et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

NanoLuc LgBit

Tags

dSaCas9

Expression

Mammalian

Promoter

CMV

Available since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pREC1010C-Eco1

Plasmid

#240690

Purpose

RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by J23115 constitutive promoter

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Eco1 RT, Eco1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010lac-Kva1

Plasmid

#240695

Purpose

RSF1010 origin of replication plasmid containing Kva1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Kva1 RT,Kva1 ncRNA, Beta and EcSSB

Expression

Bacterial

Mutation

WT

Available since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pREC1010lac-Ban1

Plasmid

#240693

Purpose

RSF1010 origin of replication plasmid containing Ban1 recombitron with a SapI flanked stuffer in the ncRNA expressed by lac promoter

Depositor

Seth Shipman

Publication

Gonzalez-Delgado et al Nat Biotechnol. 2026 Apr 23. doi: 10.103

Insert

Ban1 RT, Ban1 ncRNA, CspRecT and EcSSB

Expression

Bacterial

Mutation

WT

Available since

Aug. 7, 2025

Availability

Academic Institutions and Nonprofits only

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HaloKu70 sgRNA

Plasmid

#207583

Purpose

sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

Depositor

Jens Schmidt

Publication

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GAGCAGTAGCCAACATGTCA

Expression

Mammalian

Available since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pB_Tet-Off_Flex_DEST_EFS_mODC_tTA (JDW 936)

Plasmid

#229828

Purpose

A Piggybac-compatible, cre-dependent, Tet-off destination vector with a downstream WPRE. EFS driving destabilized tTA

Depositor

Joshua Wythe

Publication

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pAV-H1promoter-2xTetO-Tornado-1xALFA-5xSunTag

Plasmid

#231597

Purpose

Express circular RNA translation reporter with 1xALFATag-5xSunTag under H1 promoter

Depositor

Marvin Tanenbaum

Publication

Madern et al Cell. 2025 Jan 23:S0092-8674(25)00045-5.

Insert

Tornado-1xALFATag-5xSunTag

Expression

Mammalian

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_iRFP670

Plasmid

#191749

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Publication

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and iRFP670

Expression

Bacterial

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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csr-1_son-1_vsfGFP9

Plasmid

#191747

Purpose

Test fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.

Depositor

Jay C Dunlap

Publication

Wang et al Fungal Genet Biol. 2022 Dec 5:103763. do

Insert

NCU04288 (NCU04288 Neurospora crassa)

Tags

NCU04502 promoter and vsfGFP-9

Expression

Bacterial

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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