We narrowed to 27,610 results for: nes
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Plasmid#120082PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI
UseOtherAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNB330
Plasmid#60147PurposeDisintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)DepositorInsertNLuc
TagsNuclear localization signal (NLS)ExpressionYeastPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB783
Plasmid#60162PurposeFirefly luciferase (Promega) driven by RNR1 promoter.DepositorInsertFLuc
ExpressionYeastMutationA4V & S504G (no functional effect)PromoterRNR1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB763
Plasmid#60158PurposeFirefly luciferase (Promega) driven by LYS9 promoter.DepositorInsertFLuc
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLYS9prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB769
Plasmid#60138PurposeRed click beetle luciferase from Promega driven by MET17 promoter.DepositorInsertCBR
ExpressionYeastPromoterMET17prAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A hu_ncOGT WT
Plasmid#154281PurposeEntry vector encoding non-tagged O-GlcNAc TransferaseDepositorAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cyto-CaNARi
Plasmid#64849PurposeCytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.DepositorInsertCaNARi
TagsCerulean, Nuclear export signal (NES), and VenusExpressionMammalianPromoterCMVAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-Cyto-CaNARi
Plasmid#64849PurposeCytosol-targeted FRET biosensor for monitoring calcineurin (CaN) activation.DepositorInsertCaNARi
TagsCerulean, Nuclear export signal (NES), and VenusExpressionMammalianPromoterCMVAvailable SinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAB2076 pAAV REPAIR.t1
Plasmid#176323PurposepAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)DepositorInsertshuman codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)
Cas13bt1 crRNA + BpiI cloning site
UseAAV and CRISPRTags3xHA and HIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterEFS (short EF1alpha) and hU6Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB2076 pAAV REPAIR.t1
Plasmid#176323PurposepAAV EFS-HIVNES-dCas13bt1- (GGS)2-huADAR2(E488Q)-3xHA BGHpolyA::U6-BpiI-Cas13bt1 DR (full REPAIR.t1 + crRNA expression for AAV production)DepositorInsertshuman codon optimized Cas13bt1 (catalytically inactivated)
huADAR2dd(E488Q) (ADARB1 Human)
Cas13bt1 crRNA + BpiI cloning site
UseAAV and CRISPRTags3xHA and HIV NESExpressionMammalianMutationE488Q, only the deaminase domain (aa 276-702 are …PromoterEFS (short EF1alpha) and hU6Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_USP7_MATH
Plasmid#178490PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM20C
Plasmid#135487PurposeAgrobacterium T-DNA with divergent Spun H2A/Bpr driving H2B-tdTomato and hphDepositorInserthph SpunH2A/Bpr H2B-tdTomato
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2A/Bpr (divergent)Available SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_EIF4E_eiF4E
Plasmid#178474PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM4941 (pCV0053)
Plasmid#120083PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 3 (NS3).DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS3-RK2BOM, CV-aacC1, CV-ccdB-SwaI
UseOtherAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2
Plasmid#149517PurposeN-termial APEX2 tag. Gateway destination vector for mammanlian expressionDepositorTypeEmpty backboneTagsFLAG-APEX2ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJJiaDEST-APEX2
Plasmid#149517PurposeN-termial APEX2 tag. Gateway destination vector for mammanlian expressionDepositorTypeEmpty backboneTagsFLAG-APEX2ExpressionMammalianPromoterCMVAvailable SinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSTBlue-U6-sgRNA(tdTomato)
Plasmid#47871PurposeThis plasmid contains expression cassette for sgRNA targeting tdTomato.DepositorInsertsgRNA-tdTomato
UseCRISPRExpressionMammalianPromoterU6Available SinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A FKBP12F36V-OGT
Plasmid#154287PurposeEntry vector encoding FKBP12(F36V)-2xHA-OGT (dTAG fused-O-GlcNAc Transferase)DepositorAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK FKBP12(F36V)-OGT (Neo)
Plasmid#154294PurposeLentiviral vector encoding FKBP12(F36V)-2xHA-OGT (Neomycin resistant) off PGK promoterDepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x HA and FKBP12(F36V)ExpressionMammalianPromoterPGKAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM4891 (pCV0003)
Plasmid#120080PurposeBroad host range plasmid. Bacteria plasmid vector to test various parts and devices. Expression of GFP and a Kanamycin/Neomycin resistance gene in various cyanobacteria.DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aph1, CV-PconII-LTRBS-GFPmut2
UseOtherMutationmobA Y25FAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only