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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,911 results for: LIC

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Showing: 3561 - 3580 of 8911 results

  2. pcDNA5-FRT-TO-FH-Nsp13_GC3opt

    Plasmid
    #157715
    Purpose
    mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FH-Nsp13_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    Flag-His8
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available since
    Aug. 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157715-plasmid-map-sequence-id-307906

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  6. 5HT2C-RNA1-FLAG

    Plasmid
    #79678
    Purpose
    serotonin receptor 2C RNA isoform 1 with C-terminal flag tag
    Depositor
    Stefan Stamm
    Publication
    Zhang et al EMBO Mol Med. 2016 Aug 1;8(8):878-94. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5HT2C-RNA1 (HTR2C Human)
    Tags
    flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  7. pEGFP-C3-PLK4-S285A/T289A-3xFLAG

    Plasmid
    #69841
    Purpose
    This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag
    Depositor
    Michel Bornens
    Publication
    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PLK4 (PLK4 Human)
    Tags
    3xFLAG tag and EGFP
    Expression
    Mammalian
    Mutation
    S285A/T289A
    Promoter
    CMV
    Available since
    Dec. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    69841-plasmid-map-sequence-id-123880

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  8. p168-759

    Plasmid
    #62619
    Purpose
    ePathBrick pETM6 vector encoding CsF3H, AaDFR, DuLAR with Protein Scaffold GBD:SH3:PDZ (1:1:4)
    Depositor
    Mattheos Koffas
    Publication
    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1
    Inserts
    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    Scaffold Protein encoding GBD:SH3:PDZ (1:1:4)
    Use
    Synthetic Biology
    Tags
    GBD, PDZ, and SH3
    Expression
    Bacterial
    Promoter
    T7
    Available since
    July 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    62619-plasmid-map-sequence-id-103510

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  9. pGL3Basic_ME.1/ApoEpromoter

    Plasmid
    #51435
    Purpose
    This plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancer
    Depositor
    Sohail Tavazoie
    Publication
    Pencheva et al Cell. 2014 Feb 27;156(5):986-1001. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ME.1+ApoE promoter -980/+(93 bp.) (APOE, ME1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    ME.1 enhancer is fused upstream of ApoE promoter …
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. NSP6-Flag

    Plasmid
    #210345
    Purpose
    Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells
    Depositor
    Michele Pagano
    Publication
    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 NSP6 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Many synonymous changes due to codon optimization
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    210345-plasmid-map-sequence-id-418203

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  11. pcDNA3-sACt-mCherry-NLS

    Plasmid
    #138214
    Purpose
    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.
    Depositor
    Jin Zhang
    Publication
    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sACt-mCherry-NLS (Adcy10 Rat)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    138214-plasmid-map-sequence-id-307416

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  12. pcDNA3-sACt-mCherry-NES

    Plasmid
    #138215
    Purpose
    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; cytosol targeted.
    Depositor
    Jin Zhang
    Publication
    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sACt-mCherry-NES (Adcy10 Rat)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    138215-plasmid-map-sequence-id-307425

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  13. pcDNA5-FRT-TO-Nsp8-Nsp7-HF_GC3opt

    Plasmid
    #157702
    Purpose
    mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp8-Nsp7 fusion protein under control of a tetracycline-inducible promoter
    Depositor
    David Tollervey
    Publication
    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nsp8-Nsp7-HF_GC3opt (ORF1ab SARS-CoV-2)
    Tags
    His8-Flag
    Expression
    Mammalian
    Mutation
    codon optimized; gene expression was optimized by…
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    157702-plasmid-map-sequence-id-307866

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  14. pCRISPR-Cas9/CD4-TK2

    Plasmid
    #104397
    Purpose
    The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.
    Depositor
    Jeronimo Blanco
    Publication
    Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TK2 (TK2 Human)
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    104397-plasmid-map-sequence-id-208338

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  15. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Publication
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

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  17. nuc-GafD-mTurboID-HA

    Plasmid
    #184641
    Purpose
    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Publication
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    nuc-GlycoID-HA
    Tags
    3xHA tag and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    GafD -> expressed 22-178 ; BirA* -> express…
    Promoter
    CMV
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184641-plasmid-map-sequence-id-366747

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  18. pCOTS-pyl-GFP(35TAG)

    Plasmid
    #92047
    Purpose
    This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.
    Depositor
    Lital Alfonta
    Publication
    Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    EGFP
    Pyrrolysyl tRNA(cua)
    Pyrrolysyl tRNA synthetase (methanosarcina mazei)
    Use
    Replicative expression plasmid for cyanobacteria …
    Tags
    Histag
    Mutation
    Tyrosine in position 35 of the GFP was mutated f…
    Promoter
    LeuP (native S. elongatus promoter), PpsbII, and …
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92047-plasmid-map-sequence-id-194905

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  20. Anti-Halobacterium GvpA scFv 2B10-1-pcDNA 3.4 TOPO

    Plasmid
    #174688
    Purpose
    Plasmid for recombinant expression in mammalian cells of an anti-Halobacterium salinarum GvpA single chain variable fragment derived from monoclonal antibody 2B10-1
    Depositor
    Jean Gariepy
    Publication
    Fernando et al ACS Pharmacol Transl Sci. 2024 Jun 3;7(6
    Insert
    Anti- Halobacterium salinarum GvpA single chain variable fragment 2B10-1
    Tags
    hemagglutinin (HA) tag, hexahistidine (HIS) tag
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV promoter
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174688-plasmid-map-sequence-id-347064

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Showing: 3561 - 3580 of 8911 results