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pUBC(k)-AT5G01640-mKOk

Plasmid

#224847

Purpose

Plasmid for expression of AT5G01640.1 coding sequence tagged with mKOk in plants

Depositor

Rebecca Roston

Article

LaBrant et al bioRxiv 2025.06.20.660776; doi: https://

Insert

AT5G01640.1 (PRA1.B5 Mustard Weed)

Expression

Plant

Available Since

July 3, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM01

Plasmid

#233473

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Nat selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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pMTKM03

Plasmid

#233475

Purpose

SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and G418 selection.

Depositor

Vincent Martin

Article

Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF5)-TS.FF5x1-bGH

Plasmid

#235277

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

June 2, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF4)-TS.FF6x1-bGH

Plasmid

#235282

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF5)-TS.FF6x1-bGH

Plasmid

#235260

Purpose

ComMAND open-loop circuit regulating mRuby2 with miRE-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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rsZACRO/pcDNA3

Plasmid

#236199

Purpose

Mammalian expression of rsZACRO, a positively reversibly photoswitchable fluorescent protein

Depositor

Takeharu Nagai

Article

Ozaki-Noma et al ACS Nano. 2025 Feb 25;19(7):7188-7201. d

Insert

rsZACRO

Expression

Mammalian

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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rsZACRO/pRSETB

Plasmid

#236200

Purpose

Bacterial expression of rsZACRO, a positively reversibly photoswitchable fluorescent protein

Depositor

Takeharu Nagai

Article

Ozaki-Noma et al ACS Nano. 2025 Feb 25;19(7):7188-7201. d

Insert

rsZACRO

Expression

Bacterial

Available Since

May 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1alpha TDH

Plasmid

#236457

Purpose

stable expression of TDH using viral infection

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

TDH (Tdh Mouse)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pUC18 HA PTPL1

Plasmid

#237335

Purpose

expression in bacterial cells for protein purification

Depositor

Michele Pagano

Article

Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

Insert

PTPL1 (PTPN13 Human)

Tags

HA

Expression

Bacterial

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA FLAG p85beta

Plasmid

#237336

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

Insert

p85beta (PIK3R2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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hPGK-mRuby2-bGH

Plasmid

#235254

Purpose

ComMAND base gene regulating mRuby2, hPGK-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

hPGK (human phosphoglycerate kinase)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miR.FF5)-TS.FF6x1-bGH

Plasmid

#235276

Purpose

ComMAND open-loop circuit regulating mRuby2 with miR-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF4)-TS.FF6x1-bGH

Plasmid

#235270

Purpose

ComMAND open-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miRE.FF4)-TS.FF4x1-bGH

Plasmid

#235271

Purpose

ComMAND closed-loop circuit regulating mRuby2, EF1a

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miR.FF4)-TS.FF4x1-bGH

Plasmid

#235283

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miR-FF4

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-TS.FF4x1-mRuby2-bGH

Plasmid

#235274

Purpose

3' microRNA target site expression for dual-vector iFFL circuit (closed-loop)

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a-mRuby2(miRE.FF5)-TS.FF5x1-bGH

Plasmid

#235261

Purpose

ComMAND closed-loop circuit regulating mRuby2 with miRE-FF5

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EF1a-tagBFP-bGH

Plasmid

#235257

Purpose

tagBFP expression to serve as a "filler" plasmid for transfections

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

tagBFP

Use

Synthetic Biology

Expression

Mammalian

Mutation

Silent mutations to remove BsaI sites in tagBFP

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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EFS-mRuby2-bGH

Plasmid

#235253

Purpose

ComMAND base gene regulating mRuby2, EFS-driven

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EFS (human EF1a short, intronless)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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