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pTWIN1-His6-Ssp-Httex1-15Q

Plasmid

#84348

Purpose

Expression of the human Huntingtin Exon1 protein containing 15Q in E.coli

Depositor

Hilal Lashuel

Article

Vieweg et al J Biol Chem. 2016 Jun 3;291(23):12074-86

Insert

Huntingtin Exon1 (HTT Human)

Tags

N-terminal Ssp intein (His-tagged)

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pACE-MBP-FBXL17 (310–701)

Plasmid

#228369

Purpose

express human FBXL17 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXL17 (FBXL17 Human)

Tags

MBP

Expression

Insect

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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Strep-His-Tev-hCGNL1

Plasmid

#236520

Purpose

For expression of Paracingulin (human) in insect cells

Depositor

Sandra Citi

Article

Rouaud et al J Cell Biol. 2023 Jul 3;222(7):e20220806

Insert

CGNL1 (CGNL1 Human)

Tags

2xStrep-10xHis-Tev

Expression

Insect

Mutation

WT

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

Plasmid

#229544

Purpose

Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

Depositor

Domitilla Del Vecchio

Article

Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

Insert

Oct4(co)-mNeonGreen (POU5F1 Human)

Use

Lentiviral

Tags

Oct4-mNeonGreen

Expression

Mammalian

Mutation

codon-optimized OCT4

Promoter

TRE12

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pRetroX-TRE3G-GMIP P363D P364D P366D

Plasmid

#221323

Purpose

Dox-inducible expression of GMIP mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG

Mutation

P363D P364D P366D

Promoter

TRE3G

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-MAVS(pLxIS) E433A

Plasmid

#221258

Purpose

Expression of MAVS pLxIS (393-441) mutant in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS pLxIS (393-441) (Mavs Mouse)

Use

Retroviral

Tags

FLAG-4xFKBP

Mutation

E433A

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS(GMIP pLxIS)

Plasmid

#221316

Purpose

Expression of MAVS chimera with GMIP pLxIS domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS chimera with GMIP pLxIS domain (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-MAVS(pLxIS trunc)

Plasmid

#221314

Purpose

Expression of MAVS truncated pLxIS domain in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS truncated pLxIS domain (Mavs Mouse)

Use

Retroviral

Tags

FLAG

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-MAVS(trunc Cterm)

Plasmid

#221259

Purpose

Expression of MAVS truncation (393-431) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS truncation (393-431) (Mavs Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(pLxIS) S365A

Plasmid

#221241

Purpose

Expression of mutant STING CTT in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Mutation

S356A

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-STING(pLxIS) L373A

Plasmid

#221242

Purpose

Expression of mutant STING CTT in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

STING CTT (Sting1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Mutation

L373A

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2-mt2

Plasmid

#220240

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2-mt1

Plasmid

#220239

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

R27A, R31A, F165A

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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NEIL3-mNeonGreen

Plasmid

#215144

Purpose

Expresses NEIL3-mNeonGreen in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

NEIL3 (NEIL3 Human)

Use

Retroviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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SS-Cyclin D3

Plasmid

#215116

Purpose

Expresses SS-Cyclin D3 in mammalian cells.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CCND3 (CCND3 Human)

Tags

SS (STREP-STREP)

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1mut-F961A_S

Plasmid

#147514

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-CIM1-F961A (gw Human, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-M2WA-CIM1-2mut-siRNAres_S

Plasmid

#147517

Purpose

Insect Expression of HsTNRC6A-M2WA-CIM1-2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-M2WA-CIM1-2mut-siRNAres (TNRC6A Human)

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_205-830-dsRNAres_S

Plasmid

#147513

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_205-830-dsRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_205-830-dsRNAres (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-CIM1-2mut-siRNAres_R

Plasmid

#147412

Purpose

Insect Expression of HsTNRC6A-CIM1-2mut-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

HsTNRC6A-CIM1-2mut-siRNAres (TNRC6A Human)

Expression

Insect

Mutation

one non silent mutation K1578E compared to the se…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-M2WA_R

Plasmid

#147387

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-M2WA

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-M2WA (gw Fly, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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