We narrowed to 5,339 results for: 290

1. pDONR223_SETD8_WT

   Plasmid

   #82290

   Purpose

   Gateway Donor vector containing SETD8, part of the Target Accelerator Plasmid Collection.

   Depositor

   William Hahn, David Root, Jesse Boehm

   Article

   Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

   Insert

   SETD8 (KMT5A Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   P316R

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pORANGE GFP-Arpc5 KI

   Plasmid

   #131503

   Purpose

   Endogenous tagging of Arp2/3 complex subunit 5: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Arpc5, Arpc5 Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE GFP-CACNA1E KI

   Plasmid

   #131481

   Purpose

   Endogenous tagging of CaV2.3, R: N-terminal (amino acid position: G5)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cacna1e, Cacna1e Rat, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available Since

   Sept. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SEPT_Cdk2-T160A-siR

   Plasmid

   #73975

   Purpose

   HDR template to generate Cdk2-T160A-siR

   Depositor

   Steven Dowdy, Manuel Kaulich

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160A (CDK2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   T160 mutated to Ala and siRNA target site mutated

   Promoter

   Available Since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SEPT_Cdk2-T160E-siR

   Plasmid

   #73976

   Purpose

   HDR template to generate Cdk2-T160E-siR

   Depositor

   Steven Dowdy, Manuel Kaulich

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160E (CDK2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   T160 mutated to Glu and siRNA target site mutated

   Promoter

   Available Since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP SNAP47

   Plasmid

   #101915

   Purpose

   Expresses SNAP47 in mammalian cells

   Depositor

   Thierry Galli

   Article

   Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

   Insert

   SNAP47 (SNAP47 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX304-YAP1 _SH3bm

   Plasmid

   #59141

   Purpose

   YAP1 mutants that harbored a deletion of SH3 binding motif, which disrupts interaction of YES1 with YAP1

   Depositor

   William Hahn

   Article

   Shao et al Cell. 2014 Jul 3;158(1):171-84. doi: 10.

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   SH3 domain deleted (aa278-290)

   Promoter

   Available Since

   Oct. 23, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pPICZ-His6-K2P4.1B-mCherry-StreptagII

   Plasmid

   #158744

   Purpose

   Expresses K2P4.1 isoform B with TEV protease cleavable N- and C-terminal affinity tags.

   Depositor

   Arthur Laganowsky

   Article

   Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

   Insert

   K2P4.1-B (KCNK4 Human)
   

   Use

   Tags

   His6 and mCherry with Streptag II

   Expression

   Yeast

   Mutation

   Residues 1-290 and N-linked glycosylation sites r…

   Promoter

   AOX1

   Available Since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

   Plasmid

   #127289

   Purpose

   all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

   Depositor

   Fred Gage

   Article

   Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

   Inserts

   Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
   Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Ngn2 and Ascl1 linked via 2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLfa-PUb-MCS

    Plasmid

    #52908

    Purpose

    Aedes aegypti polyubiquitin promoter followed by MCS

    Depositor

    Zach Adelman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ae aegptyi polyubiquitin

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR_mCherry

    Plasmid

    #229078

    Purpose

    Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1s

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pIVTR(T7G)-PuroR

    Plasmid

    #172290

    Purpose

    In vitro transcription of Puro resistance gene (pac)

    Depositor

    Patrik Verstreken

    Insert

    PuroR
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter G-initiating

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-5X-1 U1-C-FL

    Plasmid

    #129077

    Purpose

    generation of GST-tagged U1-C protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    U1-C (SNRPC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV CAG Barcode7

    Plasmid

    #229069

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    CAG Barcode7
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV CAG Barcode6

    Plasmid

    #229068

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    CAG Barcode6
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV CAG Barcode4

    Plasmid

    #229065

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    CAG Barcode4
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV syn Barcode1

    Plasmid

    #229062

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    Syn Barcode1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV CAG Barcode5

    Plasmid

    #229067

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    CAG Barcode5
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV CAG Barcode3

    Plasmid

    #229064

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    CAG Barcode3
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV CAG Barcode2

    Plasmid

    #229063

    Purpose

    Expression mapping

    Depositor

    Scott Sternson

    Insert

    CAG Barcode2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge