We narrowed to 17,199 results for: por

1. Fluorescent reporter for MOBE3-4

   Plasmid

   #219946

   Purpose

   Mammalian expression of 2x-deadEGFP(A111V/L202S) and aptamer-gRNAs to correct both mutations for MOBE3-4

   Depositor

   Alexis Komor

   Article

   Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

   Insert

   CMV-EGFP(A111V/L202S); U6-A111V-gRNA-com-3'end; U6-L202S-gRNA-MS2-SL3
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   EGFP(A111V/L202S)

   Promoter

   CMV, U6

   Available Since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-REPORT(PGK)

   Plasmid

   #172328

   Purpose

   LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   d2eGFP
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3x SV40 NLS

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA3_GAL4UAS-Luciferase reporter

   Plasmid

   #64159

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Sport6 Baf60b

   Plasmid

   #24723

   Depositor

   Benoit Bruneau

   Article

   Lickert et al Nature. 2004 Nov 4. 432(7013):107-12.

   Insert

   Baf60b (Smarcd2 Mouse)
   

   Expression

   Bacterial and Mammalian

   Available Since

   Aug. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pORANGE Cplx2-GFP KI

   Plasmid

   #131476

   Purpose

   Endogenous tagging of Complexin2: C-terminal (amino acid position: L128)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cplx2 Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pmiR-report-hEZH2

   Plasmid

   #112543

   Purpose

   3'UTR reporter assay

   Depositor

   Shusheng Wang

   Article

   Zhou et al Mol Ther. 2014 Feb;22(2):378-389. doi: 1

   Insert

   EZH2 (EZH2 Human)
   

   Use

   Luciferase

   Available Since

   July 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. OMM PKA Reporter

   Plasmid

   #104550

   Purpose

   Expresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.

   Depositor

   David Lawrence

   Article

   O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

   Insert

   Tom20 - mTurquoise2 - VASP 148-164
   

   Tags

   mTurqouise2

   Expression

   Mammalian

   Promoter

   EF1 alpha

   Available Since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pORANGE CACNG2-GFP KI

   Plasmid

   #131504

   Purpose

   Endogenous tagging of Tarpγ2/Stargazin: Intramolecular (amino acid position: S265)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Cacng2, Cacng2 Mouse, Rat)
   

   Expression

   Mammalian

   Promoter

   U6 and Cbh

   Available Since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNeurog2-Luciferase reporter

   Plasmid

   #64126

   Purpose

   Photoactivatable transcription system. Luciferase reporter under the control of the Neurog2 promoter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   Neurog2 promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Promoter

   Neurog2

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pORANGE WASF1-GFP KI

    Plasmid

    #131502

    Purpose

    Endogenous tagging of WASP1/WAVE1: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Wasf1 Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pmiReport SHIP1 3' UTR

    Plasmid

    #26531

    Depositor

    David Baltimore

    Article

    O'Connell et al Proc Natl Acad Sci U S A. 2009 Apr 28. 1

    Insert

    SHIP1 3'UTR (Inpp5d Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pSCIPpay-BFP reporter

    Plasmid

    #154096

    Purpose

    Self-Cutting and Integrating Payload CRISPR backbone with BFP reporter

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    P2A-tagBFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA1_GAL4UAS-Luciferase reporter

    Plasmid

    #64157

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA1 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgRNA2_GAL4UAS-Luciferase reporter

    Plasmid

    #64158

    Purpose

    Photoactivatable transcription system. Lentiviral expression of sgRNA2 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA2 for GAL4UAS-Luciferase reporter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pORANGE GSG1l-GFP KI

    Plasmid

    #131492

    Purpose

    Endogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Gsg1l Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+methylated-reporter

    Plasmid

    #164491

    Purpose

    Reporter to test methylated mRNA stability, for mRNA injection

    Depositor

    Antonio Giraldez

    Article

    Kontur et al Cell Rep. 2020 Dec 29;33(13):108598. doi

    Insert

    A-less CDS, HA tag, 12x m6A motif 3'UTR
    

    Use

    Production of mrna for zebrafish injection.

    Tags

    HA tag

    Promoter

    SP6

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pORANGE CACNG8-GFP KI #1

    Plasmid

    #131473

    Purpose

    Endogenous tagging of Tarpγ8: Intramolecular (amino acid position: V376)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Cacng8 Rat)
    

    Expression

    Mammalian

    Promoter

    U6 and Cbh

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV_Donor/Reporter-COL4A5

    Plasmid

    #130280

    Purpose

    COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

    Depositor

    Silvestro Conticello

    Article

    Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

    Insert

    mCherry-eGFP
    

    Use

    AAV

    Promoter

    CMV

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTricHis-ILY-prepore

    Plasmid

    #160519

    Purpose

    Expression of inserted ILY variant

    Depositor

    Doryen Bubeck

    Article

    Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

    Insert

    ILY-prepore
    

    Tags

    His-Tag

    Expression

    Bacterial

    Mutation

    I104C, G244C

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CRISPR-Sa-TdTom. Reporter

    Plasmid

    #99652

    Purpose

    Red fluorescent reporter that can be activated by dSa Cas9 activator indicating activity

    Depositor

    George Church

    Insert

    RFP
    

    Expression

    Mammalian

    Mutation

    dead Cas9

    Available Since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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