We narrowed to 548 results for: prime editing

1. pGL3-U6-sgRNA_SRD5A1-mcherry

   Plasmid

   #159780

   Purpose

   S. pyogenes sgRNA collocated with pegRNA targeting human SRD5A1 gene

   Depositor

   Xiaolong Wang

   Article

   Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

   Insert

   spacer of sgRNA targeting SRD5A1 gene (SRD5A1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-HD118

   Plasmid

   #172655

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD118A/pegRNA-HD118B
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-Sp-gRNA-IDS2_DF_A4b_attP_fwd

   Plasmid

   #182149

   Purpose

   To insert attP-fwd attachment site at IDS2 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS2-A4b-attP-fwd pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-Sp-gRNA-IDS_DF_D1b_attB_rev

   Plasmid

   #182152

   Purpose

   To insert attB-rev attachment site at IDS in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS-D1b-attB-rev pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-Sp-gRNA-IDS_DF_C2c_attB_rev

   Plasmid

   #182151

   Purpose

   To insert attB-rev attachment site at IDS in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS-C2c-attBrev pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pU6-Sp-gRNA-IDS2_DF_B7b_attP_fwd

   Plasmid

   #182150

   Purpose

   To install attP-fwd attachment site at IDS2 in human cells via twinPE

   Depositor

   David Liu

   Article

   Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

   Insert

   IDS2-B7b-attP-fwd pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   March 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pp-CBBC-Fluc

   Plasmid

   #232920

   Purpose

   Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.

   Depositor

   Philippe Mangeot

   Article

   Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lentiguide-Blast-eNMU710

   Plasmid

   #172658

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 710-bp deletion on e-NMU locus

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-eNMUA/pegRNA-eNMUB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-HD252

   Plasmid

   #172656

   Purpose

   Expressing paired pegRNAs from human U6 and H1 promoters to make 252-bp deletion on HPRT1 gene

   Depositor

   Jay Shendure

   Article

   Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

   Insert

   pegRNA-HD252A/pegRNA-HD252B
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-PE2-C

    Plasmid

    #164908

    Purpose

    Expresses the C-terminal split-intein fragment of PE2

    Depositor

    Wen Xue

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    PE2-C
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-hyPE2

    Plasmid

    #177173

    Purpose

    Expresses hyPE2 in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Song et al Nat Commun. 2021 Sep 23;12(1):5617. doi:

    Insert

    hyperPE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-PE2-N

    Plasmid

    #164909

    Purpose

    Expresses the N-terminal split-intein fragment of PE2

    Depositor

    Wen Xue

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    PE2-N
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. evoBxb1

    Plasmid

    #222338

    Purpose

    Plasmid for expressing evolved Bxb1 in the mammalian cells

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved Bxb1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_gRNA -hMLHdn-Puro

    Plasmid

    #191105

    Purpose

    Backbone for lentiviral pegRNA library construction with hMLH1dn and Puro marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    I31 silent mutation (ATA to ATC) to remove early …

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. Factor IX donor

    Plasmid

    #182141

    Purpose

    Factor IX donor for twinPE and Bxb1 mediated insertion at ALB

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-Factor IX intron 1 and exon 2-8 CDS and exon 8 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiV2-EF1α-nCas9/RT

    Plasmid

    #210188

    Purpose

    Expression of nCas9/RT-P2A-Puro

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Insert

    nCas9/RT-P2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pG3H-PE3max-attR1R2

    Plasmid

    #213049

    Purpose

    Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Inserts

    Cas9 nickase
    M-MLV Reverse Transcriptase
    

    Use

    Note: this plasmid needs helper pvs1-vir2 for rep…

    Tags

    Expression

    Plant

    Mutation

    H840A, R240K, N413K,

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. QPM-sgR (pTaU3)

    Plasmid

    #140448

    Purpose

    For sgRNA expression in monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    TaU3-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    TaU3

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. evoBxb1_IVT

    Plasmid

    #222341

    Purpose

    Plasmid for evolved Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized evolved Bxb1
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. wtBxb1_IVT

    Plasmid

    #222340

    Purpose

    Plasmid for wild type Bxb1 mRNA in vitro transcription template

    Depositor

    David Liu

    Article

    Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

    Insert

    Codon-optimized wtBxb1
    

    Use

    Template for in vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T7 (inactivated)

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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