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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,240 results for: spl

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Showing: 341 - 360 of 4240 results

  2. Spike Display_Part 2 DO

    Plasmid
    #172722
    Purpose
    Encodes a sfGFP dropout expression cassette in place of Part 2 of HexaPro-D614G spike to be used in the Spike Display system
    Depositor
    IIya Finkelstein
    Publication
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S HexaPro-D614G (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    172722-plasmid-map-sequence-id-344857

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  3. USPL1cat-pEYFP_C1

    Plasmid
    #85766
    Purpose
    mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion
    Depositor
    Frauke Melchior
    Publication
    Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    USPL1, catalytic domain (aa 212-514) (USPL1 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    catalytic domain (aa 212-514)
    Promoter
    CMV
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85766-plasmid-map-sequence-id-169509

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  5. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Publication
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  10. Spike Display_B.1.427/B.1.429

    Plasmid
    #172738
    Purpose
    Expression vector for SARS-CoV-2 (B.1.427/B.1.429) spike used in the Spike Display system
    Depositor
    IIya Finkelstein
    Publication
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 S B.1.427/B.1.429 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available since
    Aug. 8, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172738-plasmid-map-sequence-id-339247

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  11. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Publication
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Expression
    Bacterial
    Promoter
    PBAD and PLtetO
    Available since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61966-plasmid-map-sequence-id-101265

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  12. pDisplay-GRAB-gDA3h-IRES-mCherry-CAAX

    Plasmid
    #208693
    Purpose
    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h and a membrane-localized mcherry in mammalian cells
    Depositor
    Yulong Li
    Publication
    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based dopamine (DA) sensor GRAB_gDA3h
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    208693-plasmid-map-sequence-id-412353

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  13. pDisplay-GRAB-rDA3m-IRES-EGFP-CAAX

    Plasmid
    #208696
    Purpose
    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3m and a membrane-localized EGFP in mammalian cells
    Depositor
    Yulong Li
    Publication
    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based dopamine (DA) sensor GRAB_rDA3m
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    208696-plasmid-map-sequence-id-412358

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  18. pDisplay-GRAB-rDA3h-IRES-EGFP-CAAX

    Plasmid
    #208697
    Purpose
    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA3h and a membrane-localized EGFP in mammalian cells
    Depositor
    Yulong Li
    Publication
    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based dopamine (DA) sensor GRAB_rDA3h
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    208697-plasmid-map-sequence-id-412359

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Showing: 341 - 360 of 4240 results