We narrowed to 3,469 results for: ttl

1. pRK5-HA-Ubiquitin-K27R

   Plasmid

   #121155

   Purpose

   Mammalian expression of HA tagged ubiquitin K27R

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K27 mutated to an R

   Promoter

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Melissa Little, Sara Howden

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK5-HA-Ubiquitin-K6R

   Plasmid

   #121153

   Purpose

   Mammalian expression of HA tagged ubiquitin K6R

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K6 mutated to an R

   Promoter

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-HA-Ubiquitin-K27R-K29R

   Plasmid

   #121156

   Purpose

   Mammalian expression of HA tagged ubiquitin K27R and K29R

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K27 and K29 mutated to Rs

   Promoter

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB9P3

   Plasmid

   #210034

   Purpose

   Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   prime editor 2
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5-HA-Ubiquitin-K11

   Plasmid

   #121152

   Purpose

   Mammalian expression of HA tagged ubiquitin with K11 only, other lysines mutated to arginines

   Depositor

   Josef Kittler

   Article

   Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All Ks apart from K11 mutated to Rs

   Promoter

   Available Since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-FLAG-HAND1-T2A-mTagBFP2

   Plasmid

   #223192

   Purpose

   Entry vector containing FLAG-HAND1 with a T2A-mTagBFP2 reporter (attL flanked)

   Depositor

   Matthew Birket

   Article

   Lynch et al EMBO J. 2025 Mar 31. doi: 10.1038/s44318

   Insert

   HAND1 (HAND1 Human)
   

   Use

   Promoterless entry vector for gateway cloning

   Tags

   FLAG and T2A-mTagBFP2

   Expression

   Bacterial

   Mutation

   Promoter

   None

   Available Since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. Anti-Gs protein, alpha subunit [N192/12]

   Plasmid

   #190302

   Purpose

   Mammalian Expression Plasmid of anti-Gs protein, alpha subunit (Cattle). Derived from hybridoma N192/12.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Gs protein, alpha subunit (Bos taurus) recombinant Mouse monoclonal antibody (GNAS Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pP150

   Plasmid

   #113201

   Purpose

   shuttle vector, N-terminal mTurquoise2 tagging

   Depositor

   Rob Kay

   Type

   Empty backbone

   Use

   Dictyostelium discoideum shuttle vector, n-termin…

   Tags

   mTurquoise2

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR_mCherry

    Plasmid

    #139554

    Purpose

    mCherry ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-rtTA3G

    Plasmid

    #139556

    Purpose

    rtTA ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    rtTA3G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR-Emerald

    Plasmid

    #139549

    Purpose

    Emerald ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    Emerald
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR-TetR

    Plasmid

    #139558

    Purpose

    TetR ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    TetR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-Venus

    Plasmid

    #139551

    Purpose

    Venus ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pENTR-tTAF2

    Plasmid

    #139557

    Purpose

    tTAF2 ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    tTAF2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-DsRedT4

    Plasmid

    #139550

    Purpose

    DsRedT4 ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    DsRedT4
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR-mKO1

    Plasmid

    #139553

    Purpose

    mKO1 ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    mKO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-d4Venus

    Plasmid

    #139552

    Purpose

    d4Venus ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    d4Venus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pENTR-CreERT2

    Plasmid

    #139547

    Purpose

    CreERT2 ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    CreERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-Cre

    Plasmid

    #139545

    Purpose

    Cre ORF with stop codon between attL1-attL2 on Gateway entry vector

    Depositor

    Minoru Ko

    Insert

    Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge