We narrowed to 20,068 results for: ato

1. pPOM031_P4_tSynth29

   Plasmid

   #216456

   Purpose

   Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Terminator synth29
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPOM030_P4_tSynth27

   Plasmid

   #216455

   Purpose

   Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Terminator synth27
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPOM029_P4_tSynth25

   Plasmid

   #216454

   Purpose

   Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Terminator synth25
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPOM028_P4_tSynth3

   Plasmid

   #216453

   Purpose

   Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Terminator synth3
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPOM027_P4_tSynthGuo

   Plasmid

   #216452

   Purpose

   Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Terminator guo
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKJ238

   Plasmid

   #212321

   Purpose

   Express KnFnuc guide with pureexpress

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   KnFunc guide
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOTTC1992 - pAAV EF1a CoV2 Orf3a-2xStrep

   Plasmid

   #213542

   Purpose

   AAV viral vector packaging plasmid that expresses CoV2

   Depositor

   Christopher Richie, Brandon Harvey

   Insert

   CoV2 Orf3a-2xStrep (ORF3a Human, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOTTC1993 - pAAV EF1a CoV2 protE-2xStrep

   Plasmid

   #213543

   Purpose

   AAV viral vector packaging plasmid that expresses CoV2

   Depositor

   Christopher Richie, Brandon Harvey

   Insert

   CoV2 protE-2xStrep (E Human, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mpeg:Rab5c-stop-polyA; cmlc2:GFP

   Plasmid

   #213737

   Purpose

   Expresses mCherry-Rab5c in the macrophage lineage of zebrafish with a green heart transgenesis marker

   Depositor

   William Talbot

   Article

   Iyer et al Sci Adv. 2022 Sep 2;8(35):eabp8321. doi:

   Insert

   mCherry-Rab5c
   

   Use

   Zebrafish

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   mpeg1.1

   Available Since

   Feb. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_Caspase-11_humanized-mutant

    Plasmid

    #214315

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (Casp4 Mouse)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    N152K_KLS288YKT_KASIHS348TPRAKA

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28a_Caspase-11_rat_catalytic domain

    Plasmid

    #214329

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (Casp4 Rat)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28_Caspase-4_W267N

    Plasmid

    #214314

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    W267N

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28_Caspase-4_R269D

    Plasmid

    #214313

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4 (CASP4 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    R269D

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28a_Pro-IL-18_lemur

    Plasmid

    #214332

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18
    

    Use

    Tags

    His-TEV and Myc

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a_Caspase-4_lemur_catalytic domain

    Plasmid

    #214328

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP4
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKJ217

    Plasmid

    #212318

    Purpose

    Express MmFnuc with pureexpress

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

    Insert

    Mercenaria
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCX:γB2ΔICD-EC5-VenusΔN3C9-P3

    Plasmid

    #209090

    Purpose

    mVenus-inserted PcdhgB2 lacking an intracellular domain (ICD)

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCX:γB2-EC5-VenusΔN3C9-P3

    Plasmid

    #209092

    Purpose

    mVenus-inserted PcdhgB2

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCX:γB2-EC1-G4S-mTQ2ΔC6

    Plasmid

    #209091

    Purpose

    mTurquoise2-inserted PcdhgB2

    Depositor

    Takeharu Nagai

    Article

    Kanadome et al Sci Rep. 2021 Nov 15;11(1):22237. doi: 1

    Insert

    PcdhgB2 (Pcdhgb2 Mouse)
    

    Use

    Tags

    PA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken β-actin

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-PRLSS-NbGFP_miRFP670nano3-KDEL

    Plasmid

    #209718

    Purpose

    Mammalian expression of NbGFP-miRFP670nano3 localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-NbGFP_miRFP670nano3-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCMV-PRLSS-Clover-KDEL

    Plasmid

    #209720

    Purpose

    Mammalian expression of clover localized to endoplasmic reticulum

    Depositor

    Stewart Loh

    Article

    Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

    Insert

    PRLSS-Clover-KDEL
    

    Use

    Tags

    PRLSS

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PlayBack-CMV-Bamh1

    Plasmid

    #203303

    Purpose

    For expression of eukaryotic genes of interest with CMV promoter with Bamh1 as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. PlayBack-CMV-Nhe1

    Plasmid

    #203304

    Purpose

    For expression of eukaryotic genes of interest with CMV promoter with NheI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PlayBack-CMV-Bgl11

    Plasmid

    #203306

    Purpose

    For expression of eukaryotic genes of interest with CMV promoter with BglII as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PlayBack-Ef1a-Nde1

    Plasmid

    #203309

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with NdeI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PlayBack-Ef1a-Nhe1

    Plasmid

    #203310

    Purpose

    For expression of eukaryotic genes of interest with Ef1a promoter with NheI as a PlayBack Restriction Enzyme

    Depositor

    Aleksandar Necakov

    Article

    Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDRF1-GW ymTq2-d11

    Plasmid

    #205490

    Purpose

    express ymTq2-d11 as donor control for yAT1.03

    Depositor

    Bas Teusink

    Article

    Luzia et al iScience. 2023 Dec 21;27(1):108767. doi:

    Insert

    ymTq2-d11
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCfuNca-MCS

    Plasmid

    #190820

    Purpose

    Multiple cloning site between Nitzschia captiva promoter and termination regions

    Depositor

    Peter Kroth

    Article

    Sprecher et al Algal Research. 2023 May; Vol 72: 103136

    Inserts

    Multiple cloning site with SpeI and PstI
    Bleomycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAAV-ef1a-DIO-eGCaMP(plus)

    Plasmid

    #201149

    Purpose

    AAV virus production for Cre recombinase dependent expression of eGCaMP(plus)

    Depositor

    Andre Berndt

    Article

    Wait et al Res Sq. 2023 Aug 7:rs.3.rs-3146778. doi:

    Insert

    eGCaMP+
    

    Use

    AAV, Cre/Lox, and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLV-REPORT(EF1a no GFP)

    Plasmid

    #172339

    Purpose

    mnucTomato

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pO5

    Plasmid

    #193749

    Purpose

    Expresses tdTomato under the control of PIAH synthetic promoter regulated by AHL and IPTG, p15A origin of replication, Chloramphenicol selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al ACS Synth Biol. 2021 Oct 15;10(10):2456-

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PIAH

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pIM63

    Plasmid

    #193545

    Purpose

    pENTRY clone for NLR Integrated Domain ID#19

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#19
    

    Use

    Pentry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pIM65

    Plasmid

    #193547

    Purpose

    pENTRY clone for NLR Integrated Domain ID#14

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#14
    

    Use

    Pentry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGWT35S-AtTIM21(1-50aa)-mCherry

    Plasmid

    #194047

    Purpose

    Transient expression of AtTIM21-mCherry in plant cell (Mitochondria)

    Depositor

    Yutaka Kodama

    Article

    Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

    Insert

    mCherry
    

    Use

    Tags

    AtTIM21 1–50aa

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pIM66

    Plasmid

    #193548

    Purpose

    pENTRY clone for NLR Integrated Domain ID#12

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#12
    

    Use

    Pentry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pIM60

    Plasmid

    #193542

    Purpose

    pENTRY clone for NLR Integrated Domain ID#22

    Depositor

    Nemo Peeters

    Article

    Landry et al Plant J. 2023 Sep;115(5):1443-1457. doi:

    Insert

    ID#22
    

    Use

    Pentry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pYPQ265N1

    Plasmid

    #173998

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-UNG for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-UNG
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pYPQ265O1

    Plasmid

    #173999

    Purpose

    Gateway entry clone (attL1 & attR5) rAPOBEC1-zCas9(D10A)-rXRCC1 for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    rAPOBEC1-zCas9(D10A)-rXRCC1
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. T7-10-QUAS-GFP_ColE1

    Plasmid

    #171657

    Purpose

    QUAS ten base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-10-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. T7-15-QUAS-GFP_ColE1

    Plasmid

    #171658

    Purpose

    QUAS fifteen base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-15-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. T7-TetO-QF_T7-LacO-TetR_p15A

    Plasmid

    #171659

    Purpose

    Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-LacO-TetR-T7 Stop
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. T7-0-QUAS-GFP_ColE1

    Plasmid

    #171650

    Purpose

    QUAS directly downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-0-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. QUAS-0-T7-CcdB_ColE1

    Plasmid

    #171652

    Purpose

    QUAS directly upstream of a T7 promoter. Expresses CcdB in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    CcdB-ssrA
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. T7-5-QUAS-GFP_ColE1

    Plasmid

    #171656

    Purpose

    QUAS five base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

    Depositor

    Tara Deans

    Article

    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

    Insert

    T7-5-QUAS
    

    Use

    Tags

    ssrA degradation tag (DAS+4)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. LDB221

    Plasmid

    #185427

    Purpose

    Bacterial expression of Nup1 C-terminal residues 777-1076 as GST fusion

    Depositor

    Ken Belanger, Laura Davis

    Article

    Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 C-terminal residues 777-1076

    Promoter

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. LDB203

    Plasmid

    #185424

    Purpose

    Bacterial expression of N-terminus of NUP1 nucleoporin as GST-Nup1-N fusion

    Depositor

    Ken Belanger, Laura Davis

    Article

    Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 N-terminal domain only

    Promoter

    Available Since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. LDB205

    Plasmid

    #185425

    Purpose

    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion

    Depositor

    Ken Belanger, Laura Davis

    Article

    Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 C-terminal domain only

    Promoter

    Available Since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pEUK036

    Plasmid

    #180828

    Purpose

    T7-inducible expression construct to produce CnGdmA (CNE_RS35785) in E. coli

    Depositor

    Gregg Beckham

    Article

    Bleem et al Chem Catalysis, 2022

    Insert

    CnGdmA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. KBB101

    Plasmid

    #185064

    Purpose

    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after the last FXFG repeat

    Depositor

    Ken Belanger

    Article

    Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

    Insert

    NUP1
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 C-terminal region, truncated

    Promoter

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. 3TERM_Ntpsba_rev_chlo

    Plasmid

    #163932

    Purpose

    L0 part - terminator

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Nicotiana tabacum psba terminator
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge