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OA-986B

Plasmid

#124999

Purpose

Expresses dCas9-VPR driven by the Ubiquitin-63E promoter

Depositor

Omar Akbari

Article

Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

Insert

dCas9-VPR

Use

Piggyback

Expression

Insect

Promoter

Ubiquitin

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGS-21a-PcAad1

Plasmid

#163039

Purpose

expression of aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

Depositor

Jean-Marie Francois

Article

Yang et al BMC Microbiol. 2012 Jun 28;12:126. doi:

Insert

aryl-alcohol dehydrogenase

Tags

GST and His6

Expression

Bacterial

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x5

Plasmid

#58775

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available Since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-IETD-NanoLuc

Plasmid

#98288

Purpose

caspase-8 BRET Sensor

Depositor

Maarten Merkx

Article

den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

Insert

mNeonGreen-LEHD-NanoLuc

Tags

His-tag

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2019

Availability

Academic Institutions and Nonprofits only

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pNVLTv2-pvdIJD-Prha-base

Plasmid

#169244

Purpose

Suicide vector for integrating expression cassette in P. putida (L-rhamnose induction)

Depositor

Brian Pfleger

Article

Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

Insert

rhaRS

Expression

Bacterial

Promoter

rhaS

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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MKK2

Plasmid

#29580

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

MKK2 (MAP2K2 Human)

Tags

GST, HIS6, and TEV

Expression

Bacterial

Mutation

Constitutively active mutant; Starting Met remove…

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pET28b::mAID

Plasmid

#15923

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

AID (Aicda Mouse)

Tags

His

Expression

Bacterial

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-Gdf2

Plasmid

#163595

Purpose

Expresses GDF-2 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Gdf2 (Gdf2 Mouse)

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ106: pRS426-His-MBP-NlovFz2_Native

Plasmid

#205253

Purpose

S. cerevisiae expression vector for NlovFz2 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ45: pRS426-His-MBP-SpuFz1_Native

Plasmid

#205252

Purpose

S. cerevisiae expression vector for SpuFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pCDB179-LCC

Plasmid

#218686

Purpose

Encodes codon optimized LCC enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

LCC

Tags

10xHis

Expression

Bacterial

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1 RagB shRNA #1

Plasmid

#26627

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

RagB shRNA (RRAGB Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET28-Rsn2

Plasmid

#61686

Purpose

bacterial expression of ranaspumin 2 as a His-tagged fusion protein

Depositor

Brian Smith

Article

Mackenzie et al Biophys J. 2009 Jun 17;96(12):4984-92. d

Insert

Ranaspumin-2

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LAP2-YFP-NLS

Plasmid

#25847

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LAP2-YFP-NLS

Tags

His and NLS

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pJCC_039 FnCas12a

Plasmid

#171666

Purpose

For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

FnCas12a

Tags

6X His and sortase A sorting motif

Expression

Bacterial

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pMJ123: pRS426-His-MBP-SpuFz1_PSP1

Plasmid

#205256

Purpose

S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagD 77L

Plasmid

#19317

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S77L

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
pVG122

Plasmid

#211765

Purpose

Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

Depositor

Sahand Jamal Rahi

Article

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

strongLOV

Use

For single-copy integration (if cut with pmei)

Tags

SV40 NLS and VP16

Expression

Yeast

Mutation

compared to EL222, strongLOV contains Glu84Asp mu…

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pIW447-CRISPR XYL2

Plasmid

#98906

Purpose

K. marxianus CRISPR Plasmid for XYL2 editing

Depositor

Ian Wheeldon

Article

Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

Inserts

Codon optimized Cas9
sgRNA expression cassette

Use

CRISPR and Synthetic Biology

Tags

SV40

Expression

Yeast

Available Since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-K1347A

Plasmid

#25365

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Lysine 1347 mutated to Alanine

Available Since

June 22, 2010

Availability

Academic Institutions and Nonprofits only