We narrowed to 4,064 results for: gast

1. TetO-FUW-MCS-EnR_C-term

   Plasmid

   #136885

   Purpose

   Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

   Depositor

   Moritz Mall

   Article

   Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

   Insert

   engrailed repressor domain (en Fly)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-DEST42 -SSRP1

   Plasmid

   #170439

   Purpose

   Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

   Depositor

   Joan Conaway

   Article

   Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

   Insert

   ssrp1 (Ssrp Fly)
   

   Tags

   6xHis and V5

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. BII-ChBtW-CTNNB1_S33Y

   Plasmid

   #133376

   Purpose

   Piggybac vector for constitutive expression

   Depositor

   Blair Madison

   Article

   Fischer et al Am J Physiol Gastrointest Liver Physiol.

   Insert

   CTNNB1 (CTNNB1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S33Y

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Jan. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Dscam1_7.27.26 EC10-Fc-His

   Plasmid

   #72189

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.26
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dscam1_7.27.23 EC10-Fc-His

   Plasmid

   #72187

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.23
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. Dscam1_7.27.26 EC10-AP-His

   Plasmid

   #72063

   Purpose

   Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.26 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Dscam1_7.27.26
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs_FLAG-Sfxn2 (CG6812)

   Plasmid

   #110643

   Purpose

   Retroviral expression of Flag-tagged Drosophila CG6812 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Science. 2018 Nov 16;362(6416). pii: 362

   Insert

   CG6812 (CG6812 Fly)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized cDNA (no amino acid mutations)

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs_FLAG-Sfxn1-3 (CG11739)

   Plasmid

   #110642

   Purpose

   Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Science. 2018 Nov 16;362(6416). pii: 362

   Insert

   CG11739 (CG11739 Fly)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized cDNA (no amino acid mutations)

   Available Since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. mei-S332 5.6Kb genomic DNA in Casper4

   Plasmid

   #112984

   Purpose

   P element vector for transposon insertion of mei-S332 5.6Kb genomic DNA into Drosophila genome

   Depositor

   Terry Orr-Weaver

   Article

   Kerrebrock et al Cell. 1995 Oct 20;83(2):247-56.

   Insert

   mei-S332 (Sgo) (mei-S332 Fly)
   

   Use

   P insertion vector

   Tags

   GFP

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX6P-1 PUM FL

    Plasmid

    #112997

    Purpose

    For protein expression and purification of Drosophila pumilio (amino acids 566-1090)

    Depositor

    Terry Orr-Weaver

    Article

    Hara et al Elife. 2018 Feb 26;7. pii: 33150. doi: 1

    Insert

    pumilio (pum Fly)
    

    Expression

    Bacterial

    Available Since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. UASp-deltaN cycB

    Plasmid

    #112967

    Purpose

    P element vector for transposon insertion of nondegradable Cyclin B into Drosophila genome

    Depositor

    Terry Orr-Weaver

    Article

    Lee et al Genetics. 2001 Aug;158(4):1545-56.

    Insert

    cycB (CycB Fly)
    

    Use

    P insertion vector

    Mutation

    N-term deletion of first 46 amino acids, includes…

    Available Since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. exon K-renilla

    Plasmid

    #81007

    Purpose

    luciferase based mini-gene to report splicing at exon 25 in Drosophila parayltic

    Depositor

    Richard Baines

    Article

    Lin et al Brain. 2015 Apr;138(Pt 4):891-901. doi:

    Insert

    paralytic (para Fly)
    

    Tags

    Renilla luciferase

    Expression

    Insect

    Mutation

    spanning exon 24 to exon 26, termination codon in…

    Promoter

    P-AC5 (Actin 5C)

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Dscam1_7.27.30 EC10-AP-His

    Plasmid

    #72064

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.30 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.30
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Dscam1_7.27.22 EC10-AP-His

    Plasmid

    #72060

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.22
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Dscam1_7.27.23 EC10-AP-His

    Plasmid

    #72061

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.23 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.23
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Dscam1_7.27.22 EC10-Fc-His

    Plasmid

    #72186

    Purpose

    Expresses the 10 N-terminal extracellular domains of the Dscam, isoform 7.27.22 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Dscam1_7.27.22
    

    Tags

    Fc-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFOSGE_COF3H04

    Plasmid

    #70971

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    Taf6 (Taf6 Fly)
    

    Use

    Gateway entry vector

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pFOSGE_COF1G05

    Plasmid

    #70809

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    CkIIbeta (CkIIbeta Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pFOSGE_COF1B07

    Plasmid

    #70819

    Purpose

    Gateway entry clone

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    CkIIalpha (CkIIalpha Fly)
    

    Use

    Gateway entry vector

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

20. KHBD00701

    Plasmid

    #39737

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6464 (salm Fly)
    

    Use

    Gateway donr vector

    Promoter

    none

    Available Since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only