We narrowed to 6,241 results for: nls

1. pBOBI C-FLAG PHGDH V261M

   Plasmid

   #212999

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   V261M

   Promoter

   cmv

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBOBI C-FLAG PHGDH T78A

   Plasmid

   #212997

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T78A

   Promoter

   cmv

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBOBI C-FLAG PHGDH R135W

   Plasmid

   #212998

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R135W

   Promoter

   cmv

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBOBI C-FLAG PHGDH T57A

   Plasmid

   #212996

   Purpose

   expresses human PHGDH

   Depositor

   Sheng-cai Lin

   Article

   Wu et al Cell Res. 2023 Nov;33(11):835-850. doi:

   Insert

   PHGDH (PHGDH Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T57A

   Promoter

   cmv

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-FlpO

   Plasmid

   #89574

   Purpose

   Expresses FlpO under pCAG

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   FlpO
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWL078 MCP-2xGFP

   Plasmid

   #197047

   Purpose

   pCYC-NLS-MCP-2xGFP on pRS305

   Depositor

   Robert Singer

   Article

   Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

   Insert

   MCP-2xGFP
   

   Expression

   Yeast

   Promoter

   CYC1

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6xHis-MBP-huMb2Cas12a

   Plasmid

   #174676

   Purpose

   Expresses 6xHis-MBP tagged Mb2Cas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huMb2Cas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTPGI_dCas9

   Plasmid

   #64279

   Purpose

   encodes yeast-optimized dCas9 synthetic transcription factor

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   Yeast-optimized dCas9
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   D10A & H840A relative to S. pyogenes Cas9

   Promoter

   pTPGI

   Available Since

   May 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMS2-GFP

   Plasmid

   #27121

   Depositor

   Robert Singer

   Article

   Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

   Insert

   MS2
   

   Tags

   EGFP, HA, and SV40 NLS

   Expression

   Mammalian

   Available Since

   Jan. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. 6xHis-MBP-huMb3Cas12a

    Plasmid

    #174677

    Purpose

    Expresses 6xHis-MBP tagged Mb3Cas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMb3Cas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNuc-TrxRFP1

    Plasmid

    #98998

    Purpose

    mammalian expression of a biosensor to monitor thioredoxin redox dynamics, nuclear localization

    Depositor

    Huiwang Ai

    Article

    Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

    Insert

    TrxRFP1 and nuclear localization sequence
    

    Tags

    His-tag and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGADCg

    Plasmid

    #20161

    Depositor

    Peter Uetz

    Article

    Stellberger et al Proteome Sci. 2010 Feb 15;8:8. doi: 10.1

    Type

    Empty backbone

    Tags

    Gal4 AD and SV40 NLS

    Expression

    Yeast

    Available Since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRS42H_PTEF1_dCas9_TCYC1

    Plasmid

    #177155

    Purpose

    Expresses dCas9 in yeast cells

    Depositor

    Gautam Dantas

    Article

    Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

    Insert

    Nuclease-null Cas9
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Promoter

    TEF1 promoter

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pC2HB

    Plasmid

    #63734

    Purpose

    bait plasmid for an adapted two-hybrid system for C. albicans

    Depositor

    Patrick Van Dijck

    Article

    Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

    Type

    Empty backbone

    Use

    C. albicans bait plasmid

    Tags

    HA, LexA, and NLS

    Promoter

    Met3

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC2HP

    Plasmid

    #63735

    Purpose

    prey plasmid for an adapted two-hybrid system for C. albicans

    Depositor

    Patrick Van Dijck

    Article

    Stynen et al Nucleic Acids Res. 2010 Oct;38(19):e184.

    Type

    Empty backbone

    Use

    C. albicans prey plasmid

    Tags

    FLAG, NLS, and VP domain

    Promoter

    Met3

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMS2-YFP

    Plasmid

    #27122

    Depositor

    Robert Singer

    Article

    Fusco et al Curr Biol. 2003 Jan 21. 13(2):161-7.

    Insert

    MS2
    

    Tags

    EYFP, HA, and SV40 NLS

    Expression

    Mammalian

    Available Since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid

    #174138

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    VRER=D1135V/G1218R/R1335E/T1337R

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-Cry2FL-VP64

    Plasmid

    #60554

    Purpose

    Expresses Cry2FL-VP64 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    Cry2FL-VP64
    

    Tags

    FLAG, HA tag, and SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. EZ-L560

    Plasmid

    #173028

    Purpose

    Encodes OptoAMP2 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Tags

    SV40 NLS

    Expression

    Yeast

    Available Since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. vHB16

    Plasmid

    #193455

    Purpose

    Expression of GFP-Cas9-NLS and gRNA in A. castellanii

    Depositor

    Chantal Abergel

    Article

    Bisio et al Nat Commun. 2023 Jan 26;14(1):428. doi:

    Insert

    tRNA-gRNA
    

    Use

    CRISPR

    Promoter

    U6

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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