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Plasmid#99109PurposeExpresses human ADSL-EGFP fusion in mammalian cellsDepositorInsertADSL (ADSL Human)
TagsEGFP (enhanced green fluorescent protein)ExpressionMammalianPromoterCMVAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX462-hPRKAA1-gRNA_B
Plasmid#74375PurposegRNA_B to knockout human AMPK alpha 1 using Cas9nDepositorAvailable SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPYtag-GFPnanobody
Plasmid#214837PurposeThis plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.DepositorInsertSPYtag-GFPnanobody
TagsHis-TEV-SUMO-ExpressionBacterialPromoterT5Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAXY0002
Plasmid#179835PurposeBinary plant vector expressing eYGFPuv and GUS, both driven by a 35S promoterDepositorTypeEmpty backboneUseBinary vector for plant transformationExpressionPlantAvailable SinceNov. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)
Plasmid#185917PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available SinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-his-BACH1 K52R
Plasmid#17643DepositorInsertBACH1 (BRIP1 Human)
TagsHis and MycExpressionMammalianMutationThe lysine residue at position 52 was changed to …Available SinceApril 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
hRAP-pEZT-BM
Plasmid#177986PurposeExpression human RAP containing a C-terminal 6xHis tag in mammalian cellsDepositorInsertLDL receptor related protein associated protein 1 (LRPAP1 Human)
Tags6xHisExpressionMammalianPromoterCMVAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-CMV-Flag-CDK13_IDG-K
Plasmid#135276PurposeGateway destination clone of CDK13 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomicsDepositorInsertFlag-CDK13 (CDK13 Human)
UseLentiviral; Gateway destinationTagsFlagExpressionMammalianPromoterCMVAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP GFP-Atg14
Plasmid#38264DepositorInsertautophagy related genes 14 (ATG14 Human)
UseRetroviralTagsEGFPExpressionMammalianPromoterLTRAvailable SinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1
Plasmid#19838DepositorAvailable SinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a LEU2
Plasmid#61923PurposeExpression of K. lactis LEU2 cloned into pFA6aDepositorInsertLEU2
UseYeast genomic targetingAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBAD.Sigma32.I54N
Plasmid#59982PurposeExpresses sigma32 I54N mutant in E. coli to render a heat-shock like cellular folding environmentDepositorInsertSigma32 I54N
ExpressionBacterialMutationI54NAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET His6 ProteinG TEV LIC cloning vector (2P-T)
Plasmid#29713DepositorTypeEmpty backboneTagsHis6-ProteinG-TEVExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-GSG2
Plasmid#23472DepositorInsertGSG2 (HASPIN Human)
UseGateway CloningAvailable SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC57BsaI
Plasmid#128859PurposeModule cloning vectorDepositorTypeEmpty backboneUseUnspecifiedAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRH051
Plasmid#171625Purpose2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreenDepositorInsertsspyCas9 sgRNA-BsmBI-Destination
mNeon
UseCRISPR and LentiviralExpressionMammalianPromoterEF1-a and U6Available SinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hHDAC6
Plasmid#21099DepositorInsertHistone Deacetylase 6 (HDAC6 Human)
ExpressionMammalianAvailable SinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-CybGam-WPRE
Plasmid#207662PurposeControl for CybSEP2DepositorInsertGamillus
UseAAVMutationHistidine 86 and 159 to alanines, inserted gamill…PromoterhSynapsinAvailable SinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCWXPG-UBI-Ngn3PDX1MafA
Plasmid#114303PurposeConstitutive expression of Ngn3, PDX1 and MafA proteins from a precursor with self-cleavable 2A peptidesDepositorInsertNgn3 (NEUROG3 Human)
UseLentiviralMutationNgn3, PDX1 and MafA proteins are expressed from a…Available SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-CDH5-mCitrine-IRES-puro
Plasmid#85143Purposelentiviral expression of human VE-cadherin, C-terminally tagged with mCitrineDepositorInsertCDH5-mCitrine (VE-cadherin, C-terminally tagged with mCitrine (CDH5 Human)
UseLentiviralTagsmCitrinePromoterCAGAvailable SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only