We narrowed to 5,142 results for: mos

1. pME TAEL2.0-5xC120-minpro (JDW 1109)

   Plasmid

   #224507

   Purpose

   Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   TAEL2.0-5xC120
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME-3xHA-BirA-T2A-mCherry-caax (JDW 889)

   Plasmid

   #224512

   Purpose

   A Gateway compatible middle entry clone containing Biotin Ligase followed by T2A and mCherry membrane reporter (biotinylation and flourescent cell membrane reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   3xHA-BirA-T2A-mCherry-Caax
   

   Use

   Gateway cloning

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox (JDW 1232)

   Plasmid

   #224522

   Purpose

   A Gateway compatible middle entry clone containing loxP flanked 3xNLS-mCherry-V5-3xSTOP cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   lox-3xNLS-mCherry-V5-SV40pA-3xstop-lox
   

   Use

   Cre/Lox

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG426GAL-ccdB-mRuby3

   Plasmid

   #166142

   Purpose

   Gateway destination vector for generation of Galactose-inductibe, C-terminal mRuby3-tagged proteins in yeast. Contains a 2um element for high copy number when in yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Type

   Empty backbone

   Tags

   mRuby3

   Expression

   Yeast

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

   Plasmid

   #208977

   Purpose

   Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. Recruited polymerase expression - pCMV-T7-B103-CC(N5) (LM2946)

   Plasmid

   #208974

   Purpose

   Recruited B103 DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   B103-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

   Plasmid

   #208971

   Purpose

   Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

   Plasmid

   #208970

   Purpose

   Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29(+exo)-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. hPCNA-C148S

   Plasmid

   #190939

   Purpose

   untagged human PCNA with a cysteine to serine mutation

   Depositor

   Brian Kelch

   Article

   Magrino et al J Biol Chem. 2023 May;299(5):104656. doi

   Insert

   Proliferating Cell Nuclear Antigen (PCNA Human)
   

   Expression

   Bacterial

   Mutation

   changed cysteine 148 to serine

   Promoter

   T7

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRRL.SIN.EF1A.JAG1-F1.GS.CAR-3G

    Plasmid

    #194462

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order & (GGGGS)3 linker (scFv). GFP-Zeo for selection & monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-JAG1 CAR
    

    Use

    Lentiviral

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRRL.SIN.EF1A.JAG1-F1.218.CAR-3G

    Plasmid

    #194460

    Purpose

    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.

    Depositor

    Ana Sofia Coroadinha

    Article

    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

    Insert

    Anti-JAG1 CAR
    

    Use

    Lentiviral

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. fru-T2A-QF2 HDR plasmid

    Plasmid

    #141099

    Purpose

    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti fru gene

    Depositor

    Leslie Vosshall

    Article

    Basrur et al Elife. 2020 Dec 7;9. pii: 63982. doi: 10

    Insert

    fru-left-HDR-arm; T2A-QF2-hsp70; 3xP3-dsRed-SV40; fru-right-HDR-arm
    

    Use

    Donor template

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2z-lmo2

    Plasmid

    #164657

    Purpose

    for the synthesis of zebrafish lmo2 mRNA

    Depositor

    Didier Stainier

    Article

    Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

    Insert

    lmo2 (lmo2 Zebrafish)
    

    Use

    Mrna synthesis

    Promoter

    SP6

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. ETS2_pLENTI-CAG-IRES-GFP

    Plasmid

    #177005

    Purpose

    Mammalian lentiviral expression vector encoding ETS2

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    ETS2 (ETS2 Human)
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. CYYR1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176986

    Purpose

    Mammalian expression vector encoding CYYR1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CYYR1 (CYYR1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CBR3_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176961

    Purpose

    Mammalian expression vector encoding CBR3 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CBR3 (CBR3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. CLDN8_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176963

    Purpose

    Mammalian expression vector encoding CLDN8 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CLDN8 (CLDN8 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. FAM207A_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176950

    Purpose

    Mammalian expression vector encoding FAM207A and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    FAM207A (C21orf70 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

    Plasmid

    #91806

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    Residues A1529-G1534 replaced with a PreScission …

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

    Plasmid

    #91807

    Purpose

    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    RPO21 (RPO21 Budding Yeast)
    

    Expression

    Yeast

    Mutation

    PreScission Protease site (LEVLFQGP) inserted bet…

    Promoter

    Endogenous Rpb1 promoter

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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