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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 3621 - 3640 of 16276 results

  1. pBa.GFP-KIF5B

    Plasmid
    #134625
    Purpose
    Mammalian expression of mouse KIF5B 376-963
    Depositor
    Marvin Bentley
    Publication
    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF5B (Kif5b Mouse)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    WT with deletion of the 377 N-terminal residues.
    Promoter
    Chicken Beta actin
    Available since
    Jan. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    134625-plasmid-map-sequence-id-267821

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  4. pGES201

    Plasmid
    #190197
    Purpose
    For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection
    Depositor
    Yuefeng Guan
    Publication
    Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7
    Insert
    spCas9
    Use
    CRISPR
    Tags
    3xFlag
    Expression
    Plant
    Mutation
    plant-codon optimized
    Promoter
    Glycine max elongation factor 1A(pM4)
    Available since
    Sept. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190197-plasmid-map-sequence-id-371938

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  6. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

    Plasmid
    #154139
    Purpose
    Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.
    Depositor
    Albert Jeltsch
    Publication
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP (DNMT3A, DNMT3L Streptococcus pyogenes, Human)
    Tags
    3x FLAG and tagBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    154139-plasmid-map-sequence-id-345683

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  8. pBBR_Cas9_sp1

    Plasmid
    #154851
    Purpose
    Expresses SpCas9 and sgRNA that targets the upp gene in Rhodobacter sphaeroides genome
    Depositor
    John van der Oost
    Publication
    Mougiakos et al Microb Cell Fact. 2019 Nov 25;18(1):204.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Spcas9 gene
    sgRNA scaffold
    Use
    CRISPR
    Tags
    6xHis
    Mutation
    Codon harmonized variant for expression in Rhodob…
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154851-plasmid-map-sequence-id-309592

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  16. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Publication
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

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  17. pBa.GFP-KIF16B tail 458-1317

    Plasmid
    #134624
    Purpose
    Mammalian expression of human KIF16B 458-1317
    Depositor
    Marvin Bentley
    Publication
    Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KIF16B (KIF16B Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    WT with deletion of the 457 N-terminal residues.
    Promoter
    Chicken Beta actin
    Available since
    April 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    134624-plasmid-map-sequence-id-267813

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  19. BB3cH_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104912
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    104912-plasmid-map-sequence-id-206066

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Showing: 3621 - 3640 of 16276 results