167,601 results

1. EasyCloneYALI- CRISPR/Cas9 Set

   Plasmid Kit

   #1000000141

   Purpose

   Contains plasmids for genome editing of Y. lipolytica via CRISPR/Cas9

   Depositor

   Irina Borodina

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

2. ND6-DdCBE-right side TALE

   Plasmid

   #157841

   Purpose

   expresses right-side TALE–DddAtox half of ND6-DdCBE in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   COX8A MTS–3xFLAG–ND6 right TALE–G1397 DddA-C–UGI–ATP5B 3'UTR
   

   Expression

   Mammalian

   Available Since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hsyn-NE2h

   Plasmid

   #208687

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

   Depositor

   Yulong Li

   Has Service

   AAV5

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-Ef1a-fDIO mCherry (AAV8)

   Viral Prep

   #114471-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-Ef1a-fDIO mCherry (#114471). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO mCherry plasmid DNA. Ef1a-driven, Flp recombinase-dependent expression of mCherry. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   EF1a

   Tags

   mCherry (Flp-dependent)

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

5. pDONR223-NEK7

   Plasmid

   #23395

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NEK7 (NEK7 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPB-cT3G-cERP2-RUNX1

   Plasmid

   #192928

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of RUNX1

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   RUNX1 (RUNX1 Human)
   

   Expression

   Mammalian

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-HP1alpha

   Plasmid

   #17652

   Depositor

   Tom Misteli

   Article

   Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

   Insert

   HP1 alpha (CBX5 Human)
   

   Tags

   GFP and His

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-OMMjRCaMP-P2A-mito4xGCaMP6f

   Plasmid

   #127872

   Purpose

   Dual expression vector for mitochondrial matrix and outer membrane Ca2+ sensors

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   Outer membrane localized jRCaMP1b - P2A - mito4xGCaMP6f
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA GSK3 beta wt pcDNA3

   Plasmid

   #14753

   Purpose

   Mammalian expression of human GSK3 beta with HA tag

   Depositor

   Jim Woodgett

   Article

   He et al Nature. 1995 Apr 13. 374(6523):617-22.

   Insert

   GSK3 beta (GSK3B Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2007

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pABpuro-BluF

    Plasmid

    #46824

    Purpose

    luciferase reporter for BmalI promoter

    Depositor

    Steven Brown

    Article

    Brown et al PLoS Biol. 2005 Oct;3(10):e338. Epub 200

    Insert

    Bmal1 (Bmal1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Promoter

    Bmal1

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT-HA-hM4D(Gi)

    Plasmid

    #45548

    Purpose

    Expresses hM4D (Gi) neuronal inhibitor

    Depositor

    Bryan Roth

    Article

    Armbruster et al Proc Natl Acad Sci U S A. 2007 Mar 20;10

    Insert

    hM4D (Gi)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Halo-VSVG-RUSH

    Plasmid

    #166907

    Purpose

    RUSH cargo: Str-Ii_IRES_VSVG-SBP-Halo

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    HaloTag
    

    Tags

    HaloTag

    Expression

    Mammalian

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOpen-ETSSB

    Plasmid

    #165549

    Purpose

    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

    Depositor

    Drew Endy, FreeGenes Project, Jennifer Molloy

    Insert

    Extreme Thermostable Single-Stranded DNA Binding Protein
    

    Use

    Synthetic Biology

    Available Since

    Aug. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCBL101-RUBY

    Plasmid

    #199723

    Purpose

    Expresses betalain biosynthesis genes under CaMV 35S promoter

    Depositor

    Kan Wang

    Article

    Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

    Insert

    35S-RUBY
    

    Expression

    Plant

    Available Since

    April 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV G-CEPIA1er

    Plasmid

    #58215

    Purpose

    Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    G-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV Puro

    Plasmid

    #68469

    Purpose

    Retroviral plasmid with multiple cloning site and puromycin resistance

    Depositor

    Tyler Jacks

    Article

    Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

    Insert

    Puromycin Resistance
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    pGK

    Available Since

    Feb. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJRH-1363 LV EF1a-CD19 IRES-EGFP

    Plasmid

    #201919

    Purpose

    2nd generation lentiviral transfer plasmid for engineering cells to express human CD19

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    CD19 (CD19 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-FAH-rtTA3G

    Plasmid

    #120309

    Purpose

    An AAV vector that expresses rtTA3G under the c-fos promoter

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Science. 2018 Apr 27;360(6387):430-435.

    Insert

    rtTA3G
    

    Use

    AAV

    Promoter

    c-fos

    Available Since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLX304

    Plasmid

    #25890

    Purpose

    3rd generation lentiviral Gateway destination vector. Blasticidin selection, V5 tag.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-FLEX-SaCas9-U6-sgRNA

    Plasmid

    #124844

    Purpose

    Vector for Cre-dependent expression of SaCas9

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    SaCas9, gRNA scaffold
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS and NLS-3xHA

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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