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pMAZe

Plasmid

#60877

Purpose

Reporter construct for lineage tracing and mosaic analysis in zebrafish.

Depositor

Julian Lewis

Publication

Collins et al Nat Methods. 2010 Mar;7(3):219-23. doi:

Inserts

hsp70l promoter
Cre
dTomato
UAS promoter
Gal4VP16

Use

Zebrafish expression

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pPROBE-GT

Plasmid

#37814

Depositor

Steven Lindow

Publication

Miller et al Mol Plant Microbe Interact. 2000 Nov;13(

Insert

Promotorless gfp reporter gene

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only
pFA6a-GFP-bleMX6

Plasmid

#33141

Purpose

genomic GFP tagging in yeast

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Inserts

bleMX6
GFP

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pEJS1099: Dual-sgRNA.Design 4

Plasmid

#159537

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Publication

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with two guide RNA cassettes

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLAMP1-miRFP670nano

Plasmid

#127435

Purpose

LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Publication

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LAMP1-miRFP670nano

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEJS477-pHAGE-TO-Spy dCas9 3XmCherry-SgRNA/Telomere-All-in-one

Plasmid

#85717

Purpose

Expresses dSpyCas9-3XmCherry and telomere-targeting Spy sgRNA

Depositor

Erik Sontheimer

Publication

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Inserts

Sp dCas9
telomere sgRNA

Use

CRISPR and Lentiviral

Tags

3XmCherry

Expression

Mammalian

Promoter

CMV-TO and U6

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pScI_dCas9-CDA

Plasmid

#108549

Purpose

Bacterial Target-AID vector

Depositor

Akihiko Kondo

Publication

Banno et al Nat Microbiol. 2018 Feb 5. pii: 10.1038/

Insert

dCas9-PmCDA1

Tags

Flag

Expression

Bacterial

Mutation

D10A and H840A for SpCas9

Promoter

lambda OR

Available since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-Bsu

Plasmid

#163911

Purpose

Expresses Bsu large fragment for affinity purification

Depositor

Paul Freemont

Publication

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

Bsu polymerase

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pJB042 (FtsZ-mEos2)

Plasmid

#49764

Purpose

Inducible expression of FtsZ-mEos2 in bacteria

Depositor

Jie Xiao

Publication

Buss et al Mol Microbiol. 2013 Sep;89(6):1099-120.

Insert

FtsZ-mEos2

Tags

mEos2

Expression

Bacterial

Promoter

T5-lac

Available since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-miniGFP2-P2A-FusionRed

Plasmid

#188734

Purpose

Express miniGFP2 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

CAG

Available since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-mCherry-SpHis5

Plasmid

#44643

Purpose

Yeast tagging vector to create gene fusion with mCherry with SpHis5 selection

Depositor

Kurt Thorn

Insert

mCherry

Expression

Yeast

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGSHU

Plasmid

#72244

Purpose

Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Publication

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
hyg
I-SceI

Expression

Bacterial

Available since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBad-Xis/Int Reset Flipper

Plasmid

#38207

Depositor

Drew Endy

Publication

Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109

Inserts

Bxb1-Excisionase
Bxb1 Integrase

Use

Synthetic Biology; Biobrick cloning vector

Promoter

pBAD

Available since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET28a-MH6-uvsY

Plasmid

#163914

Purpose

Expresses uvsY for bacterial expression and affinity purification

Depositor

Paul Freemont

Publication

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsY (uvsY Enterobacteria phage T4)

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT-MBIS

Plasmid

#35150

Depositor

Jay Keasling, Taek Soon Lee

Publication

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Promoter

LacUV5,

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-miniDq-mCherry-P2A-HA-KORD

Plasmid

#204359

Purpose

AAV vector for coexpression of miniDq and KORD under the control of human synapsin promoter

Depositor

Masao Doi

Publication

Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

Insert

miniDq-mCherry-P2A-HA-KORD

Use

AAV

Tags

HA (for KORD) and mCherry (for miniDq)

Mutation

For miniDq, the third intracellular loop (ICL3) o…

Promoter

hSyn

Available since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pET32-MCM2/7

Plasmid

#116949

Purpose

Expresses human MCM2 and human MCM7

Depositor

James Chong

Publication

Hesketh et al J Biol Chem. 2015 Mar 20;290(12):7973-9.

Inserts

Human MCM7 (MCM7 Human)
Human MCM2 (MCM2 Human)

Tags

Thioredoxin, His6

Expression

Bacterial

Available since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro cPLA2-EGFP

Plasmid

#162568

Purpose

Used as a measure of cPLA2 activation in live cells with an EGFP tag.

Depositor

Philipp Niethammer

Publication

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES-Ap2a2-FKBP

Plasmid

#46944

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Publication

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap2a2-FKBP

Tags

Mainly mouse Ap2a2 cDNA, with a fragment of brain…

Expression

Mammalian

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-Ef1a-fDIO-mCherryP2ACre

Plasmid

#161773

Purpose

Flp-Dependent mCherry and Cre expression

Depositor

Baoji Xu

Publication

An et al Nat Commun. 2020 Apr 7;11(1):1729. doi:

Insert

mCherry-P2A-Cre

Use

AAV

Expression

Mammalian

Promoter

Ef1a

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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