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pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro

Plasmid

#174118

Purpose

Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro

Use

Lentiviral

Promoter

mU6 and UbC

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pTopo RE2 Donor pLox hPGK-Puro pA pLox

Plasmid

#171049

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE2

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pTopo RE3 Donor pLox hPGK-Puro pA pLox

Plasmid

#171050

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE3

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-CDKL1_IDG-K

Plasmid

#158176

Purpose

Gateway destination clone of CDKL1 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-CDKL1 (CDKL1 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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FoxFTVC+bpFOG_MRAS-G22V

Plasmid

#107521

Purpose

Constitutive activation of FGF/MAPK pathway by M-Ras-G22V mutant

Depositor

Lionel Christiaen

Article

Razy-Krajka et al Elife. 2018 Feb 6;7. pii: 29656. doi: 10

Insert

M-Ras protein

Mutation

changed Glycine 22 to Valine

Promoter

TVC-specific FoxF enhancer (FoxFTVC+bpFOG)

Available Since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)

Plasmid

#132637

Purpose

expresses mouse Tankyrase1 ARC2-3 (308-655) in E. coli

Depositor

Wenqing Xu

Article

Morrone et al Proc Natl Acad Sci U S A. 2012 Jan 31;10

Insert

Tankyrase1 (308-655)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pSTAP-seq_fly_spike-in

Plasmid

#125151

Purpose

Vector to measure CP candidates (spike-in) expression by determining the abundance of transcripts originating from the candidate by a D.pse enhancer in fly cells

Depositor

Alexander Stark

Article

Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

Insert

D. pseudoobscura peak #47 enhancer

Use

Stap-seq screening vector

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PTE_25

Plasmid

#111638

Purpose

Funclib design PTE_25

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_25

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_26

Plasmid

#111639

Purpose

Funclib design PTE_26

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_26

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_27

Plasmid

#111640

Purpose

Funclib design PTE_27

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_27

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_28

Plasmid

#111641

Purpose

Funclib design PTE_28

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_28

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_36

Plasmid

#111642

Purpose

Funclib design PTE_36

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_36

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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PTE_27.14

Plasmid

#111643

Purpose

Funclib design PTE_27.14

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

PTE_27.14

Tags

MBP

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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ACS_PROSS

Plasmid

#111645

Purpose

PROSS stabilized ACS

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_PROSS

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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ACS_4

Plasmid

#111646

Purpose

Funclib design ACS_4

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_4

Tags

6xHis

Expression

Bacterial

Mutation

see depositor comments below

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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ACS_9

Plasmid

#111647

Purpose

Funclib design ACS_9

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_9

Tags

6xHis

Expression

Bacterial

Promoter

pTac

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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ACS_10

Plasmid

#111648

Purpose

Funclib design ACS_10

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_10

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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ACS_14

Plasmid

#111649

Purpose

Funclib design ACS_14

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_14

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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ACS_16

Plasmid

#111650

Purpose

Funclib design ACS_16

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_16

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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ACS_27

Plasmid

#111652

Purpose

Funclib design ACS_27

Depositor

Sarel Fleishman

Article

Khersonsky et al Mol Cell. 2018 Oct 4;72(1):178-186.e5. d

Insert

ACS_27

Tags

6xHis

Expression

Bacterial

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge