We narrowed to 6,241 results for: nls

1. 6xHis-MBP-huPdCas12a

   Plasmid

   #174682

   Purpose

   Expresses 6xHis-MBP tagged PdCas12a

   Depositor

   Piyush Jain

   Article

   Nguyen et al medRxiv 2021.07.21

   Insert

   huPdCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis, MBP, TEV and NLS, 6xHis, 3xHA

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHdCas9-mammalian

   Plasmid

   #138415

   Purpose

   Expression of Himar-dCas9 in mammalian cells

   Depositor

   Harris Wang

   Article

   Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

   Insert

   Himar-dCas9
   

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. RHD_exon4_TALEN_L

   Plasmid

   #62241

   Purpose

   expression of TALEN targeting human RHD exon4

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

   Insert

   human RHD_exon4_TALEN_Left
   

   Use

   TALEN

   Tags

   HA tag, NLS

   Promoter

   CMV

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. RHD_exon4_TALEN_R

   Plasmid

   #62242

   Purpose

   expression of TALEN targeting human RHD exon4

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Commun. 2015 Jun 16;6:7451. doi: 10.

   Insert

   human RHD_exon4_TALEN_Right
   

   Use

   TALEN

   Tags

   HA tag, NLS

   Promoter

   CMV

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. p35ST

   Plasmid

   #51514

   Purpose

   pMDC32 with T30 TALEN pair followed by the us:NPTII donor molecule

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   T30 TALEN pair
   us:NPTII repair template
   

   Use

   Plant expression t-dna

   Tags

   NLS and flag

   Promoter

   2x35S

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRETightBI-RY-SLC6A1

   Plasmid

   #31471

   Depositor

   Phil Sharp

   Article

   Mukherji et al Nat Genet. 2011 Aug 21;43(9):854-9. doi:

   Insert

   eYFP and mCherry
   

   Tags

   NLS

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

7. p35SZ.D

   Plasmid

   #51512

   Purpose

   pMDC32 with Zif268:FokI followed by us:NPTII repair template sequence

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   Zif268:FokI
   us:NPTII repair template
   

   Use

   Plant expression t-dna

   Tags

   NLS

   Promoter

   2x35S

   Available Since

   May 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGH1110

   Plasmid

   #223682

   Purpose

   U6-CloningSite-Ef1a-Cas9-NLS-Flag-T2A-EGFP-P2A-BlastR

   Depositor

   Gaelen Hess

   Article

   Liila-Fogarty et al ACS Synth Biol. 2024 Oct 19. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ-Un1Cas12f1

   Plasmid

   #205143

   Purpose

   Un1Cas12f1 Gateway entry plasmid

   Depositor

   Yiping Qi

   Article

   Tang et al Plant Genome. 2024 May 28:e20465. doi: 1

   Insert

   Un1Cas12f1
   

   Use

   CRISPR

   Tags

   NLS

   Available Since

   June 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAN052

    Plasmid

    #220046

    Purpose

    Mammalian expression of type III-A Csm complex from Streptococcus thermophilus without NLS tags

    Depositor

    Blake Wiedenheft

    Article

    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1

    Insert

    FLAG-Csm1; FLAG-Csm2; FLAG-Csm3; FLAG-Csm4; FLAG-Csm5; FLAG-Cas6; crRNA; GFP
    

    Expression

    Mammalian

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHHYTK109

    Plasmid

    #200135

    Purpose

    Type 4a part for Modular Cloning in Yeast

    Depositor

    Johannes Herrmann

    Article

    Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

    Insert

    BPSV40-NLS
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHHYTK108

    Plasmid

    #200134

    Purpose

    Type 3a part for Modular Cloning in Yeast

    Depositor

    Johannes Herrmann

    Article

    Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

    Insert

    BPSV40-NLS
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. 6xHis-MBP-huMlCas12a

    Plasmid

    #174678

    Purpose

    Expresses 6xHis-MBP tagged MlCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huMlCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. 6xHis-MBP-huCMaCas12a

    Plasmid

    #174679

    Purpose

    Expresses 6xHis-MBP tagged CMaCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huCMaCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. 6xHis-MBP-huPxCas12a

    Plasmid

    #174675

    Purpose

    Expresses 6xHis-MBP tagged PxCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPxCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis-MBP-huPrCas12a

    Plasmid

    #174674

    Purpose

    Expresses 6xHis-MBP tagged PrCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huPrCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6xHis-MBP-huBfCas12a

    Plasmid

    #174672

    Purpose

    Expresses 6xHis-MBP tagged BfCas12a

    Depositor

    Piyush Jain

    Article

    Nguyen et al medRxiv 2021.07.21

    Insert

    huBfCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis, MBP, TEV and NLS, 6xHis, 3xHA

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid

    #174139

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-mScarlet

    Expression

    Mammalian

    Mutation

    VRQR=D1135V/G1218R/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNJB99

    Plasmid

    #51521

    Purpose

    Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Insert

    Zif268:FokI
    

    Use

    Gateway entry vector

    Tags

    NLS

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGGD006

    Plasmid

    #48836

    Purpose

    Provides the SV40 nuclear localization signal as GreenGate C-terminal tag module.

    Depositor

    Jan Lohmann

    Article

    Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

    Insert

    SV40 NLS
    

    Use

    Golden gate compatible cloning vector

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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