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px458_Conc2

Plasmid

#134449

Purpose

Empty concatemer vector in which 2 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET_GFP-SZ2

Plasmid

#124931

Purpose

Expression of GFP-SZ2

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

GFP-SYNZIP2

Tags

GFP and His

Expression

Bacterial

Mutation

SZ2 domain

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-perox-mEm

Plasmid

#185597

Purpose

Lentiviral construct for expression of mEmerald targeted to peroxisomes

Depositor

Jarrod Johnson

Article

Greiner et al Curr Protoc. 2022 Aug;2(8):e514. doi: 10

Insert

perox-mEm

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-cGAS g2 Cas9-T2A-mCherry

Plasmid

#164253

Purpose

Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgcGAS

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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MRAS

Plasmid

#55661

Purpose

expression of mouse MRAS

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

MRAS (Mras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pCMVMagFRET-1-NLS

Plasmid

#50748

Purpose

Expresses MagFRET-1 in the nucleus of mammalian cells. FRET sensor based on Cerulean/Citrine for Mg2+ imaging. Sensor does not respond to normal Ca2+ levels.

Depositor

Maarten Merkx

Article

Lindenburg et al PLoS One. 2013 Dec 2;8(12):e82009. doi:

Insert

gene encoding for MagFRET-1 with NLS

Expression

Mammalian

Available Since

Jan. 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-LIC

Plasmid

#120247

Purpose

For LIC cloning with N-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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px458_Conc3

Plasmid

#134450

Purpose

Empty concatemer vector in which 3 sgRNAs can be inserted; with Cas9 + GFP

Depositor

Daniel Stange

Article

Gaebler et al J Vis Exp. 2020 Feb 18;(156). doi: 10.37

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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Rac1

Plasmid

#55692

Purpose

Expression of mouse Rac1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

Rac1 (Rac1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pDest490-OPN-b

Plasmid

#17591

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-b (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 1–175, 217–942; See Depositor Comments

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pFUW mEmerald-P2A-NeoR

Plasmid

#239569

Purpose

Cytosolic expression of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pU6-cGAS g3 Cas9-T2A-mCherry

Plasmid

#164254

Purpose

Transcription of cGAS guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sgcGAS

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-Muc2D1D2D3cysD1

Plasmid

#155214

Purpose

Expresses human MUC2 residues 21-1397

Depositor

Deborah Fass

Article

Javitt et al Cell. 2020 Oct 29;183(3):717-729.e16. do

Insert

Muc2 D1D2D3cysD1 (MUC2 Human)

Tags

6xHistadine

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pET_RFP-SZ2

Plasmid

#124933

Purpose

Expression of RFP-SZ2

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-SYNZIP2

Tags

His and mCherry

Expression

Bacterial

Mutation

SZ2 domain

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBFv-UAS3

Plasmid

#138399

Purpose

10xUAS expression vector with a vermilion marker and a phiC31 attB sequence

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Type

Empty backbone

Expression

Insect

Promoter

UAS

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA_RFP-SZ2

Plasmid

#124936

Purpose

Expression of RFP-SZ2

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-SYNZIP2

Tags

mCherry

Expression

Mammalian

Mutation

SZ2 domain

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET19b+H2B

Plasmid

#157809

Purpose

Expression of human histone H2B

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

H2B

Expression

Bacterial

Promoter

T7

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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CDC42

Plasmid

#55693

Purpose

expression of mouse CDC42

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

CDC42

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pSG5-p110α-WT

Plasmid

#55709

Purpose

eukaryotic expression of Flag tagged p110a WT

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

p110 alpha

Tags

flag

Expression

Mammalian

Promoter

T7

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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