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pDM_T7_HisLacZomega

Plasmid

#118815

Purpose

T7 RNAP-driven expression of N-terminal His-tagged lacZ omega subunit.

Depositor

Alexander Green

Article

Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

Insert

lacZ_

Tags

histag

Expression

Bacterial

Available Since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-Spop

Plasmid

#128872

Purpose

For mammalian expression of mouse Spop

Depositor

Aimin Liu

Article

Cai et al Proc Natl Acad Sci U S A. 2016 Dec 20;11

Insert

Spop (Spop Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-GUCY2C

Plasmid

#116749

Purpose

Lentiviral expression of GUCY2C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

GUCY2C (GUCY2C Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-RagA(T21N)

Plasmid

#99711

Purpose

Mammalian expression of HA-RagA(T21N)

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Insert

RagA (RRAGA )

Tags

HA

Mutation

T21N

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:Xen5

Plasmid

#88902

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + LuxABCDE

Expression

Bacterial

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only
MSCV Puro-CCSP:GFP

Plasmid

#68487

Purpose

Retrovirus expressing GFP driven by CCSP promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

CCSP and LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1-LATS2

Plasmid

#166474

Purpose

Expresses fusion of mEGFP and LATS2

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP, LATS2 (LATS2 Human)

Expression

Mammalian

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR221-TCF7

Plasmid

#79498

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TCF7 CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

TCF7 (TCF7 Human)

Use

Gateway Cloning

Promoter

no Promoter

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Expression

Mammalian

Promoter

CAG

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-TRE-DsRed-IRES-d24EGFP

Plasmid

#41945

Purpose

Retroviral expression of DsRed in mammalian cells

Depositor

Stephen Elledge

Article

Yen et al Science. 2008 Nov 7;322(5903):918-23. do

Insert

DsRed

Use

Retroviral

Expression

Mammalian

Promoter

TRE

Available Since

May 2, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-ULK3

Plasmid

#23646

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

ULK3 (ULK3 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
CMV-miR-31 sponge

Plasmid

#25025

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

CMV-miR-31 sponge

Expression

Mammalian

Available Since

July 7, 2010

Availability

Academic Institutions and Nonprofits only
HPIV2-V-FLAG

Plasmid

#44905

Depositor

Curt Horvath

Article

Parisien et al J Virol. 2002 May;76(9):4190-8.

Insert

HPIV2 (V )

Expression

Mammalian

Available Since

Sept. 20, 2013

Availability

Academic Institutions and Nonprofits only
pDualIN-SMN2

Plasmid

#214430

Purpose

Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the inclusion of the frame-shifting cassette exon 7

Depositor

Chaolin Zhang

Article

Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

Insert

SMN2 EXON 7 (SMN2 Human)

Expression

Mammalian

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 CMV mCherry-eGFP BGH

Plasmid

#92280

Purpose

Control FRET construct; tandem mCherry-eGFP fusion

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

mCherry-eGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLZ12Km2-P23R:TA:ffluc

Plasmid

#88900

Purpose

Toxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expression

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

toxin-antitoxin + firefly luciferase

Use

Luciferase

Expression

Bacterial

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGFP

Plasmid

#64904

Purpose

"Fluorescent reporter containing no disulfide bonds/Selenocystamine folding"

Depositor

Stephen Mayfield

Article

Ferreira-Camargo et al AMB Express. 2015 Dec;5(1):126. doi: 10.

Insert

Green fluorescent protein

Use

Psba chlamydomonas reinhardtii vector

Promoter

psbA

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only
pVRb20_992

Plasmid

#49714

Depositor

Christopher Voigt

Article

Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

Insert

sfgfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

Pecf20_992

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-Kif2c

Plasmid

#208651

Purpose

Mammalian expression plasmid expressing eGFP-Kif2c driven by CMV promoter

Depositor

Samuel Bakhoum

Article

Li et al Nature. 2023 Aug;620(7976):1080-1088. do

Insert

Kif2c (KIF2C Human)

Tags

eGFP

Expression

Mammalian

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSH824-ssr

Plasmid

#198027

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

T0 terminator - Apr resistance cassette

Expression

Bacterial

Promoter

AprR promoter

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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