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pAcBac3-pAAFRS-8xYtR-EGFP-3TAA- 40TGA-152TAG-TEV-3xUAA

Plasmid

#228788

Purpose

EcTyr aaRS/tRNA system for pAAF incorporation with EGFP containing three stop codons for triple suppression

Depositor

Abhishek Chatterjee

Article

Osgood et al Angew Chem Int Ed Engl. 2023 May 2;62(19

Inserts

EcTyrRS
EcYtR
EGFP***
TEV protease

Use

Baculoviral

Expression

Mammalian

Mutation

TAG suppressor

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-ALK-R1275Q

Plasmid

#116111

Purpose

Lentiviral expression of ALK R1275Q

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ALK (ALK Human)

Use

Lentiviral

Mutation

R1275Q

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-Q546K

Plasmid

#116527

Purpose

Lentiviral expression of PIK3CA Q546K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

Q546K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-N581S

Plasmid

#116188

Purpose

Lentiviral expression of BRAF N581S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

N581S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP2K1-K57N

Plasmid

#116427

Purpose

Lentiviral expression of MAP2K1 K57N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

K57N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-AMBRA1-3xFLAG

Plasmid

#174159

Purpose

PiggyBac vector to stably express 3xFLAG-tagged AMBRA1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

AMBRA1 (AMBRA1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1A

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-L62R

Plasmid

#116265

Purpose

Lentiviral expression of EGFR L62R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

L62R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-MAP2K1

Plasmid

#116757

Purpose

Lentiviral expression of MAP2K1

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

MAP2K1 (MAP2K1 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-NRAS-K135N

Plasmid

#116443

Purpose

Lentiviral expression of NRAS K135N

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NRAS (NRAS Human)

Use

Lentiviral

Mutation

K135N

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-R108K

Plasmid

#116292

Purpose

Lentiviral expression of EGFR R108K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

R108K

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-S467L

Plasmid

#116194

Purpose

Lentiviral expression of BRAF S467L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

S467L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-Q546R

Plasmid

#116530

Purpose

Lentiviral expression of PIK3CA Q546R

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

Q546R

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-A289D

Plasmid

#116228

Purpose

Lentiviral expression of EGFR A289D

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

A289D

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G469A

Plasmid

#116153

Purpose

Lentiviral expression of BRAF G469A

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G469A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-G719A

Plasmid

#116253

Purpose

Lentiviral expression of EGFR G719A

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Mutation

G719A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BMPR2

Plasmid

#116718

Purpose

Lentiviral expression of BMPR2

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BMPR2 (BMPR2 Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-PIK3CA-H1047L

Plasmid

#116499

Purpose

Lentiviral expression of PIK3CA H1047L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PIK3CA (PIK3CA Human)

Use

Lentiviral

Mutation

H1047L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-RHOA-Y42C

Plasmid

#116673

Purpose

Lentiviral expression of RHOA Y42C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

RHOA (RHOA Human)

Use

Lentiviral

Mutation

Y42C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G596S

Plasmid

#116159

Purpose

Lentiviral expression of BRAF G596S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G596S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-hCCND1-T286A-HA

Plasmid

#174155

Purpose

Lentiviral vector expressing HA-tagged human cyclin D1 with T286A mutation

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

hemagglutinin (HA)

Expression

Mammalian

Mutation

A>G at base 856 (Thr>Ala at amino acid 286)

Promoter

EF1A

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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