We narrowed to 11,632 results for: Vars

1. pCPP6450

   Plasmid

   #128731

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Wei et al Cell Host Microbe. 2015 Jun 10;17(6):752

   Insert

   HopAD1
   

   Expression

   Bacterial

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. LENC

   Plasmid

   #111163

   Purpose

   miR-E (miR-30 variant)-based RNAi with mCherry reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Insert

   miR-E (miR-30 variant)
   

   Use

   Retroviral

   Mutation

   WT

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CaMKIIa-Archer1-EGFP

   Plasmid

   #60423

   Purpose

   Lentiviral vector with CaMKIIa promoter and upstream CMV promoter expressing Archer1-EGFP fusion. Archer1's red fluorescence monitors changes in membrane voltage.

   Depositor

   Viviana Gradinaru

   Article

   Flytzanis et al Nat Commun. 2014 Sep 15;5:4894. doi: 10.

   Insert

   Archer1-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CaMKIIa

   Available Since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1-Ls-AChBP

   Plasmid

   #63126

   Purpose

   Expression of Lymnaea stagnalis AChBP

   Depositor

   Titia Sixma

   Article

   Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

   Insert

   AChBP
   

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDAS12342_PEAR-GFP_2in1

   Plasmid

   #177184

   Purpose

   PEAR-GFP plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

   Plasmid

   #159803

   Purpose

   Expression construct with ubiquitous expression to test fluorescence level on microscope

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
   

   Expression

   Worm

   Mutation

   Not applicable

   Available Since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. xCas9(3.6)-BE4

   Plasmid

   #108386

   Purpose

   Mammalian expression vector for xCas9(3.6)-BE4

   Depositor

   David Liu

   Article

   Hu et al Nature. 2018 Feb 28. pii: nature26155. d

   Insert

   xCas9(3.6)-BE4
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYDE007

   Plasmid

   #194152

   Purpose

   sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

   Depositor

   Edward Geisinger

   Article

   Bai et al J Bacteriol. 2021 May 20;203(12):e005652

   Insert

   sgRNA with mrfp control guide sequence
   

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PGK+ plasmid with onboard mCherry cassette

   Plasmid

   #169745

   Purpose

   PGK+ plasmid that also encodes separate mCherry cassette. mCherry is used to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   PGK-tetO2

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. p4A8_S7T_BC

    Plasmid

    #217832

    Purpose

    4A8 variant in barcoded, yeast surface display plasmid

    Depositor

    Timothy Whitehead

    Article

    Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

    Insert

    4A8 variant Fab
    

    Expression

    Yeast

    Mutation

    VL: S7T

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. xCas9(3.6)-BE3

    Plasmid

    #108385

    Purpose

    Mammalian expression vector for xCas9(3.6)-BE3

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.6)-BE3
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS

    Plasmid

    #124705

    Purpose

    Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLS

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    MCP_ADAR2 DD (E488Q)_NLS
    

    Use

    AAV

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutant

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAB076i5

    Plasmid

    #79199

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1+2+3); Ppro1

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDest-303

    Plasmid

    #159678

    Purpose

    Gateway destination vector for mammalian protein expression

    Depositor

    Dominic Esposito

    Article

    Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MPRA Empty Vector

    Plasmid

    #204865

    Purpose

    Vector used for high-throughput cloning and subsequent transfection of MPRA libraries into mammalian cells.

    Depositor

    Philipp Maass

    Article

    Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

    Type

    Empty backbone

    Expression

    Bacterial and Mammalian

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-sbGLuc-ChR2(CS/DA)-EYFP

    Plasmid

    #114106

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, Channelrhodopsin-2 variant C128S/D156A (stabilized step function opsin), and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al J Neurosci Res. 2019 Apr 7. doi: 10.1002

    Insert

    Gaussia luciferase, slow burn; Channelrhodopsin 2(CS/DA), stable step function opsin; EYFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Sf-U7snRNA-ESE-AS-NsiI

    Plasmid

    #190694

    Purpose

    Basic vector used for the expression of U7-based antisense oligonucleotides

    Depositor

    Pim Pijnappel

    Article

    van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

    Insert

    U7 snRNA
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U7 promoter

    Available Since

    April 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAD-mKate(KPS12)

    Plasmid

    #133831

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Expression

    Bacterial

    Promoter

    Pupp

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Dlx-FLEX-hM4Di-mCherry

    Plasmid

    #209701

    Purpose

    Expresses hM4Di-mCherry under the control of distalless homeobox 5 and 6 (mDlx) enhancer in Cre-recombinase-dependent manner

    Depositor

    Peer Wulff

    Article

    Rangel Guerrero et al Neuron. 2024 Apr 10:S0896-6273(24)00197-

    Insert

    hM4D(Gi)
    

    Use

    AAV

    Tags

    mCherry

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAB078d8

    Plasmid

    #79206

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    PlacZ-opt (OR1+2) luxAB; Ppro1 434cI-SH2ABL1
    

    Expression

    Bacterial

    Promoter

    PlacZ-opt (OR1+2); Ppro1

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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