167,601 results

1. pCMV-Frankenbody-FLAG-mRuby2

   Plasmid

   #175294

   Purpose

   Track mature and nascent FLAG tagged proteins in live cells

   Depositor

   Tim Stasevich

   Article

   Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

   Insert

   anti-FLAG frankenbody fused with mRuby2
   

   Tags

   mRuby2

   Expression

   Mammalian

   Available Since

   Oct. 7, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pcDNA3β-FLAG-CBP-HA

   Plasmid

   #32908

   Depositor

   Tso-Pang Yao

   Article

   Zhao et al Mol Cell Biol. 2005 Oct;25(19):8456-64.

   Insert

   CBP (Crebbp Mouse)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. SF-SON IRES Puro

   Plasmid

   #214402

   Purpose

   Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

   Depositor

   Tanja Kuhlmann

   Article

   Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-fDIO-Cre (AAV8)

   Viral Prep

   #121675-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-EF1a-fDIO-Cre (#121675). In addition to the viral particles, you will also receive purified pAAV-EF1a-fDIO-Cre plasmid DNA. EF1a-driven, Flp recombinase dependent expression of Cre recombinase. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Esteban Engel, Alexander Nectow

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Promoter

   EF1a

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

5. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (AAV1)

   Viral Prep

   #51084-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from AAV-hSyn1-GCaMP6s-P2A-nls-dTomato (#51084). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6s-P2A-nls-dTomato plasmid DNA. GCaMP6s calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Jonathan Ting

   Promoter

   Syn

   Tags

   nuclear dTomato (physically separate, not a fusion protein)

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

6. pTripz Sox4.965

   Plasmid

   #101120

   Purpose

   shRNA Vector

   Depositor

   Joan Massague

   Article

   David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

   Insert

   Sox4.965
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCX-TRI-2A

   Plasmid

   #74674

   Purpose

   mammalian expression of TRI-2A (hHO1/F2A/hCD73/F2A/hCD39)

   Depositor

   Roberto Giovannoni

   Article

   Cinti et al PLoS One. 2015 Oct 29;10(10):e0141933. d

   Inserts

   HO1 (HMOX1 Human)
   CD73 (NT5E Human)
   CD39 (ENTPD1 Human)
   

   Tags

   F2A

   Expression

   Mammalian

   Promoter

   pCAGGS

   Available Since

   Feb. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Gene Trap - 24XPP7 Lentiviral Vector

   Plasmid

   #174199

   Purpose

   Lentiviral expression vector coding for Blasticidin, iRFP and 24X PP7 hairpins. All inserts are cloned in on the -strand to allow for splicing in when targeted into an intron of transcribed genes.

   Depositor

   Daniel Larson

   Article

   Wan et al Cell. 2021 May 27;184(11):2878-2895.e20.

   Insert

   iRFP
   

   Use

   Lentiviral; Vector

   Tags

   24X PP7 hairpins, Blasticidin, and T2A

   Expression

   Mammalian

   Available Since

   Sept. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. 726-Desmoglein2-myc

   Plasmid

   #32232

   Depositor

   Kathleen Green

   Article

   Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

   Insert

   Desmoglein2 (DSG2 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   human beta-actin

   Available Since

   Nov. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

10. GFP scFv [N86/8]

    Plasmid

    #199429

    Purpose

    Mammalian expression of GFP scFv. Derived from hybridoma N86/8.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targetingGFP (Aequorea victoria)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMM178

    Plasmid

    #61270

    Purpose

    Also referred to as pRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains a pBBR1 origin and chloramphenicol resistance.

    Depositor

    Timothy Lu

    Article

    Citorik et al Nat Biotechnol. 2014 Sep 21. doi: 10.103

    Insert

    Cas9, tracrRNA, crRNA
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    pLtetO, native, native (respectively)

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVX-Tight-Puro FLAG-SMAD4 TetON

    Plasmid

    #107129

    Purpose

    Inducible expression of Flag-tagged SMAD4 in mammalian cells

    Depositor

    Joan Massague

    Article

    David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

    Insert

    SMAD4
    

    Use

    Lentiviral

    Tags

    FLAG

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. NET5s pEGFP-N2 (1154)

    Plasmid

    #62034

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

    Insert

    NET5s (TMEM201 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    short form

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

14. Pprm1-luciferase; his-3

    Plasmid

    #137728

    Purpose

    Communication-activated luciferase in Neurospora crassa

    Depositor

    Louise Glass

    Article

    Fischer et al Genetics. 2019 Apr;211(4):1255-1267. doi

    Inserts

    Luciferase
    Pprm-1 Promoter
    

    Use

    Luciferase; Neurospora crassa expression

    Mutation

    Codon-optimized for N. crassa

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-C1-AR V7

    Plasmid

    #86856

    Purpose

    Fluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)

    Depositor

    Marco Marcelli, Michael Mancini

    Article

    Szafran et al Prostate. 2017 Jan;77(1):82-93. doi: 10.

    Insert

    Androgen receptor (AR) (AR Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Alternative splice variant 7 (alteration/deletion…

    Promoter

    CMV

    Available Since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMC-1-AtUbi10-6

    Plasmid

    #197700

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    AtUbi10 promoter
    

    Expression

    Plant

    Available Since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. hPlekhm1-dsRedM

    Plasmid

    #73592

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    dsRed

    Expression

    Mammalian

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

18. mEos3-Rac1

    Plasmid

    #92039

    Purpose

    Expresses mEos3-Rac1 fusion protein

    Depositor

    Ji Yu

    Article

    Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

    Insert

    RAC1 (RAC1 Human)
    

    Tags

    mEos3

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    June 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHyo094 (eMutaT7)

    Plasmid

    #173124

    Purpose

    Expresses eMutaT7 for mutagenizing genes downstream from a T7 promoter in e.coli

    Depositor

    Seokhee Kim

    Article

    Park et al Nucleic Acids Res. 2021 Apr 6;49(6):e32.

    Insert

    pmCDA1-T7RNAP
    

    Expression

    Bacterial

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. MinLibCas9

    Pooled Library

    #164896

    Purpose

    Human genome-wide CRISPR-Cas9 gRNA library, optimised by mining existing large-scale screening datasets, which provides a >60% reduction in size while preserving key information content

    Depositor

    Mathew Garnett

    Article

    Gonçalves et al Genome Biol. 2021 Jan 21;22(1):40. doi:

    Expression

    Mammalian

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only