Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 63,044 results for: cloning

Clear keywords
Showing: 3701 - 3720 of 63044 results

  1. TOM20*-SBP-GFP

    Plasmid
    #120173
    Purpose
    Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)
    Depositor
    Juan Bonifacino
    Publication
    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d
    Insert
    Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)
    Tags
    SBP and eGFP
    Expression
    Mammalian
    Mutation
    Truncated TOM20: 1-30 aa
    Promoter
    CMV
    Available since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    120173-plasmid-map-sequence-id-247521

    Enlarge

  5. rBiFC Kit

    Plasmid kit
    #1000000147
    Purpose
    Plasmids for analysis of protein-protein interactions in planta via ratiometric Bimolecular Fluorescence Complementation (rBiFC).
    Depositor
    Christopher Grefen
    Application
    Cloning and Synthetic Biology, Gene Expression and Labeling
    Vector type
    Plant Expression
    Cloning type
    Gateway
    Available since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only

  8. pDNA2.0 6H-FLAG-TEV-KRAS G12C

    Plasmid
    #111848
    Purpose
    Expresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Publication
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12C (KRAS Human)
    Tags
    6xHis-tag, FLAG-tag, and TEV protease cleavage se…
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Cysteine
    Promoter
    T5
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    111848-plasmid-map-sequence-id-213454

    Enlarge

  10. SUV[SET]-dCas9

    Plasmid
    #100088
    Purpose
    Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Insert
    SUVAR39H1 (SUV39H1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-SUV[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    deleted aa 1-76
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100088-plasmid-map-sequence-id-197559

    Enlarge

  11. pCRISPRi_Mxi1_yl

    Plasmid
    #91248
    Purpose
    CRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertion
    Depositor
    Ian Wheeldon
    Publication
    Schwartz et al Biotechnol Bioeng. 2017 Aug 19. doi: 10.
    Inserts
    Codon optimized dCas9-Mxi1
    sgRNA expression cassette
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    SCR1'-tRNA and UAS1B8-TEF(136)
    Available since
    Aug. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    91248-plasmid-map-sequence-id-192174

    Enlarge

  20. SRC_HUMAN_D0

    Plasmid
    #79700
    Purpose
    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.
    Depositor
    John Chodera, Nicholas Levinson, Markus Seeliger
    Publication
    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/
    Insert
    SRC (SRC Human)
    Tags
    His10-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
Showing: 3701 - 3720 of 63044 results