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We narrowed to 151,823 results for: ins

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Showing: 3701 - 3720 of 151823 results

  5. pPB-VPR-dSpCas9[StaPL(TI)]

    Plasmid
    #111508
    Purpose
    Expresses a VPR transcriptional activator fused to the nuclease-deactivated S. pyogenes Cas9, which is governed by an inserted StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].
    Depositor
    Michael Lin
    Article
    Jacobs et al Nat Methods. 2018 Jul;15(7):523-526. doi
    Insert
    VPR-dSpCas9[StaPL(TI)]
    Expression
    Mammalian
    Mutation
    The HCV NS3 protease carries F43L, Q80K, and S122…
    Promoter
    PGK
    Available Since
    July 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    111508-plasmid-map-sequence-id-214274

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  7. pKan cpRBP-FL P53

    Plasmid
    #174504
    Purpose
    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)
    Depositor
    Stewart Loh
    Article
    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do
    Insert
    P53 gene (TP53 Human)
    Tags
    20 amino acid linker containing HRV3C protease si…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174504-plasmid-map-sequence-id-344828

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  16. pKK-BI16-FLAG-3C-ORF1_mClover3-TEV-ORF2

    Plasmid
    #105801
    Purpose
    Concomitant expression of two proteins. One protein expressed with FLAG, cleavable by 3C or enterokinase; second protein expressed with mClover3, cleavable by TEV protease; tags positions: N-termini.
    Depositor
    Andrzej Dziembowski
    Article
    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do
    Type
    Empty backbone
    Use
    Flp-in competent
    Tags
    ORF1: FLAG-3C; ORF2: mClover3-TEV
    Expression
    Mammalian
    Available Since
    Feb. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    105801-plasmid-map-sequence-id-203395

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  18. pCAG-hAsCas12a-RR(S542R/K607R)-NLS(nucleoplasmin)-3xHA (AAS1081)

    Plasmid
    #114094
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RR(S542R/K607R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RR (S542R/K607R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R and K607R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114094-plasmid-map-sequence-id-220561

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Showing: 3701 - 3720 of 151823 results