We narrowed to 9,540 results for: pho;

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His-GFP-Rab3a

Plasmid

#170863

Purpose

Mammalian expression of His-GFP-Rab3a.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Rab-3a (Rab3a Mouse)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427T

Plasmid

#81035

Purpose

Mammalian expression of LOV I427T, a mutant with super short relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427T

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427T

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pPyCAG-mKate2-3xNLS-IRES-Pac

Plasmid

#183618

Purpose

Mammalian expression vector for mKate2-3xNLS from the CAG promoter. Confers puromycin resistance.

Depositor

Sally Lowell

Article

Malaguti et al Elife. 2013 Dec 17;2:e01197. doi: 10.755

Insert

mKate2-3xNLS

Tags

3xNLS

Expression

Mammalian

Promoter

CAG

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLz37_CB_operon_CsoS2_N1-N4

Plasmid

#140856

Purpose

Halothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D

Expression

Bacterial

Available Since

Aug. 3, 2020

Availability

Academic Institutions and Nonprofits only

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mEos4a-N1

Plasmid

#54811

Purpose

Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Type

Empty backbone

Tags

mEos4a

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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5HT6-YFP-PIPK

Plasmid

#96810

Purpose

cilia-membrane-targeted phosphatidylinositol 4-phosphate 5-kinase

Depositor

Takanari Inoue

Article

Phua et al Cell. 2017 Jan 12;168(1-2):264-279.e15.

Insert

5HT6-YFP-PIPK

Tags

YFP

Expression

Mammalian

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pKanCMV-mClover3-10aa-H2B

Plasmid

#74257

Purpose

Mammalian expression of N-terminal fusion of mClover3 to H2B

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

H2B

Tags

mClover3

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pARF6-YFP

Plasmid

#11389

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Tags

YFP

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc12-HiBiT

Plasmid

#198308

Purpose

Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc12-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)-mSandy2

Plasmid

#177760

Purpose

Expresses mSandy2 using the T7 Promoter

Depositor

Roberto Chica

Article

Legault et al Chem Sci. 2022 Jan 11;13(5):1408-1418. d

Insert

mSandy2

Tags

6xHis tag

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLC-ZsGreen-P2A-Blast

Plasmid

#123322

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Blast cassette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

ZsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

Plasmid

#198450

Purpose

Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10

Expression

Bacterial

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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Puro-T2A-mCitrine-hSmad2-donor

Plasmid

#203663

Purpose

Donor plasmid for CRISPR knock-in

Depositor

Ali Hemmati Brivanlou

Article

Yoney et al Elife. 2018 Oct 12;7:e38279. doi: 10.755

Insert

Smad2

Tags

mCitrine

Expression

Mammalian

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pRT802

Plasmid

#68113

Purpose

Integration vector derived from Streptomyces phage phiBT1. Kanamycin resistance gene, oriT, ColE1 origin of replication.

Depositor

Maggie Smith

Article

Gregory et al J Bacteriol. 2003 Sep;185(17):5320-3.

Inserts

aac3(IV)
aph11
oriT
attP
int

Use

Streptomyces integrating vector

Available Since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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ACPPiso1-V5

Plasmid

#113052

Purpose

Expresses human prostatic acid phosphatase (isoform 1) in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

prostatic acid phosphatase isoform 1 (ACP3 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pTrex-Luc-Neo

Plasmid

#48336

Purpose

Trasgenically expresses firefly luciferase in T. cruzi with neomycin selection

Depositor

Rick Tarleton

Article

Sanchez-Valdez et al Elife. 2018 Mar 26;7. pii: 34039. doi: 1

Insert

firely luciferase

Use

Trypanasoma cruzi expression

Promoter

T. cruzi rRNA

Available Since

July 20, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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Fus-BcLOV-mCh-SOS

Plasmid

#208275

Purpose

Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Article

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-SOS

Use

Lentiviral

Promoter

SFFV

Available Since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSin_EF1a_CreN59_nMag_NLS_P2A_mCh_IRES_PuroR

Plasmid

#176310

Purpose

Expresses CreN59-nMag-NLS with mCherry marker.

Depositor

Yingxiao Wang

Article

Allen et al ACS Synth Biol. 2019 Oct 18;8(10):2359-2

Insert

CreN59_nMag_NLS_P2A_mCherry_IRES_puroR

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

human EF1alpha

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_LifeAct-iBlueberry

Plasmid

#73176

Purpose

Expressing iBlueberry and mCherry in mammalian cells

Depositor

Xiaokun Shu

Article

Yu et al Protein Sci. 2016 Feb;25(2):308-15. doi:

Insert

LifeAct-iBlueberry

Expression

Mammalian

Mutation

mutation E177Q in iBlueberry

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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ACPPiso2-V5

Plasmid

#113053

Purpose

Expresses human prostatic acid phosphatase (isoform 2) in mammalian cells

Depositor

Federico Sesti

Article

Cotella et al FASEB J. 2013 Apr;27(4):1381-93. doi: 10

Insert

prostatic acid phosphatase isoform 2 (ACP3 Human)

Tags

V5

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pQL47 EGFP-VAMP-GST

Plasmid

#122971

Purpose

Reporter of botulinum toxin B cleavage

Depositor

Chandra Tucker

Article

Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

Insert

EGFP-VAMP3-GST

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pluc2-iRFP720

Plasmid

#98887

Purpose

Mammalian expression

Depositor

Laura Mezzanotte

Article

Mezzanotte et al Cell Transplant. 2017 Apr 26. doi: 10.37

Insert

Firefly Luciferase

Tags

iRFP720

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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GFP hSmad4-HomDonor

Plasmid

#206044

Purpose

Donor plasmid for CRISPR knock-in

Depositor

Ali Hemmati Brivanlou

Article

Nemashkalo et al Development. 2017 Sep 1;144(17):3042-305

Insert

Smad4 (SMAD4 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pDR111_mannose_Bgal no NLS

Plasmid

#188399

Purpose

Expresses beta-galactosidase by D-mannose promoter for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

Depositor

Christopher Contag

Article

Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

Insert

lacZ

Tags

PhoD secretion peptide

Expression

Bacterial

Promoter

D-mannose

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-CAR-GECO1

Plasmid

#45493

Purpose

Carmine intensiometric genetically encoded Ca2+-indicators for optical imaging

Depositor

Robert Campbell

Article

Wu et al ACS Chem Neurosci. 2013 Mar 19.

Insert

CAR-GECO1

Expression

Mammalian

Mutation

R-GECO1 E163V/I166V/V174T/M176I/F222I/A302P

Promoter

CMV

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pEF6 VPS52-V5-6His

Plasmid

#175096

Purpose

V5 tagged VPS52 is expressed in mammalian cells

Depositor

Juan Bonifacino

Article

Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

Insert

VPS52 (VPS52 Human)

Tags

V5

Expression

Mammalian

Mutation

N666D- please see depositor comment

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBz15_Strep_Rubisco

Plasmid

#140839

Purpose

wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

Form IA Rubisco; CbbL, CbbS

Tags

Strep II

Expression

Bacterial

Promoter

T7

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-ZsGreen-P2A-Hygro

Plasmid

#124301

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

ZsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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882 pSG5L HA PTEN G129E

Plasmid

#10746

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Expression

Mammalian

Mutation

G129E

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only

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35S-DsREDmonomer-NosT

Plasmid

#79183

Purpose

Transient expression vector for DsRED monomer

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

Type

Empty backbone

Use

Reporter plasmid

Tags

DsREDmonomer

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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