We narrowed to 8,513 results for: sgrna

1. pJMP1290

   Plasmid

   #119267

   Purpose

   construction intermediate

   Depositor

   Carol Gross, Oren Rosenberg, Jason Peters

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA NT1/RR1 (rfp)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4

   Plasmid

   #123658

   Purpose

   DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LentiCRISPR v2-sgAMPK alpha1 clone2

   Plasmid

   #162120

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGMC00013

   Plasmid

   #172525

   Purpose

   sgRNA against mouse Mr1

   Depositor

   Raj Chari

   Article

   Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

   Insert

   Mr1 (Mr1 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGMC00014

   Plasmid

   #172526

   Purpose

   non targeting sgRNA

   Depositor

   Raj Chari

   Article

   Ruf et al Cancer Immunol Res. 2021 Jun 30. pii: 23

   Insert

   Non-targeting guide
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-Puro-SIK2

   Plasmid

   #138696

   Purpose

   Expresses a human SIK2-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgSIK2 human (SIK2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-Puro-SIK3

   Plasmid

   #138697

   Purpose

   Expresses a human SIK3-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgSIK3 human (SIK3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JPF0535a

   Plasmid

   #124042

   Purpose

   Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

   Depositor

   Hana El-Samad

   Article

   Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

   Insert

   PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSECC-sgLKB1-A

   Plasmid

   #138663

   Purpose

   Expresses a mouse LKB1-targeting sgRNA, Cas9, and Cre-recombinase

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgLKB1 mouse (Stk11 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   March 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9

    Plasmid

    #87917

    Purpose

    Gateway cloning

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgHTT1, sgGFP2, shCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1, U6, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJMP1102

    Plasmid

    #119246

    Purpose

    knockdown phzA1 in P. aeruginosa

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA phzA1 (P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJMP1103

    Plasmid

    #119247

    Purpose

    knockdown phzM in P. aeruginosa

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA phzM (P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJMP1055

    Plasmid

    #119242

    Purpose

    construction intermediate

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCRISPR-Cas9/CD4-TK2

    Plasmid

    #104397

    Purpose

    The designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.

    Depositor

    Jeronimo Blanco

    Article

    Meca-Cortes et al Mol Ther Nucleic Acids. 2017 Sep 15;8:39

    Insert

    TK2 (TK2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Direct-seq_lentiGuide-Puro-Tail-8A8G

    Plasmid

    #157981

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-Hygro-sgTom

    Plasmid

    #138698

    Purpose

    Expresses a tomato-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgTomato
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p2T CAG dCas9-10XGcn4-P2A-mCherry BlastR

    Plasmid

    #186745

    Purpose

    dCas9-10XGcn4-P2A-mCherry expression construct

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    Cas9-10XGcn4-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJMP1239

    Plasmid

    #119263

    Purpose

    knockdown folA in P. aeruginosa

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA folA (P. aeruginosa)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgCRY2

    Plasmid

    #197457

    Purpose

    Expresses sgRNA(CRY2 gene) in mammalian cells

    Depositor

    Han Kyoung Choe

    Article

    Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

    Insert

    hU6-sgCRY2-1-hU6-sgCRY2-2 (Cry2 Mouse)
    

    Use

    AAV

    Tags

    mCherry (K14E, N210D, E249G)

    Expression

    Mutation

    Promoter

    U6, hSyn

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJMP1346

    Plasmid

    #119274

    Purpose

    knockdown folA in K. pneumoniae

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA folA (K. pneumoniae)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAAV-CMV-dSa VP64 Actc1

    Plasmid

    #99691

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGL3-U6-pegRNA_FANCF-EGFP

    Plasmid

    #159785

    Purpose

    S. pyogenes pegRNA for C to A mutation at the FANCF site of human cells using prime editing

    Depositor

    Xiaolong Wang

    Article

    Liu et al Cell Discov. 2020 Apr 28;6:27. doi: 10.1

    Insert

    spacer of pegRNA targeting FANCF gene (FANCF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAVsc.U6-sgFah.CMV/CB-EGFP

    Plasmid

    #121508

    Purpose

    Expresses sgRNA targeting mouse Fah intron 7 (sgFah).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJMP1344

    Plasmid

    #119273

    Purpose

    knockdown folA in E. aerogenes

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA folA (E. aerogenes)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJMP1106

    Plasmid

    #119249

    Purpose

    construction intermediate

    Depositor

    Carol Gross, Oren Rosenberg, Jason Peters

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CMV-dSa VP64 Neurog2

    Plasmid

    #99695

    Purpose

    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VP64

    Expression

    Mutation

    dead Cas9

    Promoter

    Available since

    Sept. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFUGW-shRIIα-RIIαWT-IRES-EGFP

    Plasmid

    #183456

    Purpose

    Replacement of endogenous rat PKA-RIIα subunits with wild-type RIIα

    Depositor

    Matthew Gold

    Article

    Church et al Elife. 2021 Oct 6;10. pii: 68164. doi: 1

    Insert

    sgRNA and Prkar2a (Prkar2a Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    shRNA-resistant mutations: C135>G, G138>A, …

    Promoter

    shRNA: H1 / gene: ubiquitin

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPN186

    Plasmid

    #91573

    Purpose

    Express sgRNA targeting human AKT3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    AKT3 (AKT3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLJM1-SLC35B2-FLAG

    Plasmid

    #154863

    Purpose

    Transient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    ctcctggtgcagtacttc => ttattagtccaatatttt

    Promoter

    CMV

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. API5-CRISPR

    Plasmid

    #157664

    Purpose

    Expresses Cas9 and sgRNA targeting API5

    Depositor

    Byung Il Lee

    Article

    Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

    Insert

    Apoptosis inhibitor 5 (API5 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPN187

    Plasmid

    #91574

    Purpose

    Express sgRNA targeting human AKT3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    AKT3 (AKT3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCW57.1 tTA SDHA

    Plasmid

    #177976

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    David Sabatini, Jessica Spinelli

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    SDHA (SDHA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations to SDHA sgRNA target

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SlutrCas9

    Plasmid

    #163795

    Purpose

    Expresses SlutrCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. ShaCas9

    Plasmid

    #163794

    Purpose

    Expresses ShaCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. LentiCRISPR v2-sgAMPK alpha1 clone3

    Plasmid

    #162121

    Purpose

    Lentiviral sgRNA plasmid targeting human AMPK alpha1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgAMPK alpha1 (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. ARB365

    Plasmid

    #124048

    Purpose

    Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAWp28-v1 scaffold

    Plasmid

    #157976

    Purpose

    pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAWp102-v2 scaffold

    Plasmid

    #157978

    Purpose

    pBT264-mutmU6p-{2xBbsI}-v2 sgRNA scaffold-{MfeI}

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAVsc.U6-sgGTTG.CMV/CB-Gluc

    Plasmid

    #121512

    Purpose

    Expresses sgRNA targeting the intron in the MADM alleles (GT and TG).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgGTTG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR221-H1-sgGFP1-7SK-sgCas9

    Plasmid

    #87908

    Purpose

    multiple sgRNA

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgCas9
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    H1, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge