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Plasmid#44529DepositorInsertHHF2-HHT2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pML107-KAE1
Plasmid#232891PurposePlasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.DepositorInsertKAE1 gRNA (KAE1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG Retro EGFP Puro
Plasmid#234463PurposeControl retroviral vector expressing Green Fluorescent Protein. Puromycin selection.DepositorInsertGreen Fluorescent Protein
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAG Retro EGFP Hygro
Plasmid#234462PurposeControl retroviral vector expressing Green Fluorescent Protein. Hygromycin selection.DepositorInsertGreen Fluorescent Protein
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 21, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pML107-ARB1
Plasmid#232888PurposePlasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.DepositorInsertARB1 gRNA (ARB1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-RER2
Plasmid#232884PurposePlasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.DepositorInsertRER2 gRNA (RER2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML104-LEU2-3
Plasmid#232894PurposePlasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.DepositorInsertLEU2 gRNA (RER2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS313_MSN5-LY00018
Plasmid#232903PurposePlasmid carrying MSN5 from LY00018 (YJM975) with its native promoter and terminator.DepositorInsertMSN5 (MSN5 Budding Yeast)
UseTagsExpressionYeastMutationG1563APromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-AVO1
Plasmid#232889PurposePlasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.DepositorInsertAVO1 gRNA (AVO1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-YFR054C
Plasmid#232900PurposePlasmid expressing Cas9 and gRNA GCTCAAAGAAACAATTAGAG which targets the YFR054C gene.DepositorInsertYFR054C gRNA (YFR054C Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-SRP14
Plasmid#232896PurposePlasmid expressing Cas9 and gRNA GAAAACAAAATGCTCAACAG which targets the SRP14 gene.DepositorInsertSRP14 gRNA (SRP14 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-TLG1
Plasmid#232897PurposePlasmid expressing Cas9 and gRNA GATGAAAACGAAGACGTGAG which targets the TLG1 gene.DepositorInsertTLG1 gRNA (TLG1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-VHT1
Plasmid#232898PurposePlasmid expressing Cas9 and gRNA TGCGGCCACACTGAAATACG which targets the VHT1 gene.DepositorInsertVHT1 gRNA (VHT1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML104-LEU2-2
Plasmid#232883PurposePlasmid expressing Cas9 and gRNA GGTAGTGTTAGACCTGAACA which targets the LEU2 gene.DepositorInsertLEU2 gRNA (LEU2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pML107-INO80
Plasmid#232890PurposePlasmid expressing Cas9 and gRNA GGAAGTAGAATCATTCGTAG which targets the INO80 gene.DepositorInsertINO80 gRNA (INO80 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromotersnR52Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUSE-URA3-Sec7-mEYFP
Plasmid#218968PurposeGene replacement plasmid to label S. cerevisiae Sec7 with mEYFPDepositorInsertSEC7-mEYFP (SEC7 Budding Yeast)
UseGene replacementTagsmEYFPExpressionMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUSE-URA-Sec7-mGold
Plasmid#218967PurposeGene replacement plasmid to label S. cerevisiae Sec7 with mGoldDepositorInsertSEC7-mGold (SEC7 Budding Yeast)
UseGene replacement vector for yeastTagsmGoldExpressionMutationPromoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Kar2-moxGFP2-HDEL
Plasmid#218970PurposeGene replacement plasmid to label S. cerevisiae Kar2 with moxGFP2DepositorInsertKAR2-moxGFP2-HDEL (KAR2 Budding Yeast)
UseGene replacement vector for yeastTagsmoxGFP2ExpressionMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUSE-URA3-Sec7-msYFP2
Plasmid#218969PurposeGene replacement plasmid to label S. cerevisiae Sec7 with msYFP2DepositorInsertSEC7-msYFP2 (SEC7 Budding Yeast)
UseTagsmsYFP2ExpressionYeastMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXR004_puroR
Plasmid#219820PurposehU6-driven pre-gRNA plasmid for CasRx applications with puromycin resistance. 5' processed DR followed by BbsI sites for guide cloning (Adapted from plasmid #10954)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T15 - T2PsB (mtagBFP2, mCherry, NeonGreen)
Plasmid#219689PurposeLevel 1 preswitched History-dependent target without promoter, switch by Bxb1, output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PsB (mtagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-RNQ1
Plasmid#181706PurposeGalactose inducible expression of RNQ1DepositorInsertRNQ1 (RNQ1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
PgyrA_gfp
Plasmid#212146PurposePlasmid expressing green fluorescent protein under constitutive promoter PgyrA control.DepositorInsertgreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
KBB96
Plasmid#185113PurposeBacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated so only one FXFG repeat remainsDepositorInsertNUP1
UseTagsGSTExpressionBacterialMutationLast FXFG domain of Nup1PromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB109
Plasmid#185066PurposeBacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after NsiI restriction siteDepositorInsertNUP1
UseTagsGSTExpressionBacterialMutationNup1 C-terminal region, truncated at NsiI restric…PromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-pHCountdown
Plasmid#182301Purposelentiviral plasmid encoding pH-Countdown and puromycin selectionDepositorInsertpH countdown
UseLentiviralTagsExpressionMutationPromoterEF-1aAvailable sinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pB2H6
Plasmid#163835PurposeTwo-hybrid system that confers spectinomycin resistance in response to substrate phosphorylation. Constitutive expression of c-src kinase, CDC37, and SHP1DepositorInsertSpecR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPro1;ProD;pLacZ-OptAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_MET16
Plasmid#166091PurposePlasmid for constituive spCas9 and tet-inducible MET16 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertMET16 (MET16 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMay 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_CPR1_1
Plasmid#166074PurposePlasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertCPR1 (CPR1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_RRT8
Plasmid#166080PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of RRT8 for double stranded break formation in yeast.DepositorInsertPromoter of RRT8 (RRT8 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_CPR1_2
Plasmid#166071PurposePlasmid for constituive spCas9 expression and tet-inducible expression of an sgRNA targeting an intergenic site near CPR1 for double stranded break formation in yeast.DepositorInsertIntergenic region near CPR1
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_GAL7
Plasmid#166089PurposePlasmid for constituive spCas9 and tet-inducible GAL7 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertGAL7 (GAL7 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_CUP1-1
Plasmid#166086PurposePlasmid for constituive spCas9 and tet-inducible CUP1-1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertCUP1-1 (CUP1-1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_GLK1
Plasmid#166090PurposePlasmid for constituive spCas9 and tet-inducible GLK1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertGLK1 (GLK1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_LHS1
Plasmid#166092PurposePlasmid for constituive spCas9 and tet-inducible LHS1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertLHS1 (LHS1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_APL3
Plasmid#166073PurposePlasmid for constituive spCas9 and tet-inducible APL1-targeting sgRNA expression for double stranded break formation in yeastDepositorInsertAPL3 (APL3 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_YBL055C
Plasmid#166077PurposePlasmid for constituive spCas9 and tet-inducible YBL055C targeting sgRNA expression for double stranded break formation in yeastDepositorInsertYBL055C (near PTC3) (YBL055C Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_YNR071C
Plasmid#166081PurposePlasmid for constituive spCas9 expression and tet-inducible expression of sgRNA binding to the promoter of YNR071C for double stranded break formation in yeast.DepositorInsertPromoter of YNR071C (YNR071C Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_SLA1
Plasmid#166075PurposePlasmid for constituive spCas9 and tet-inducible SLA1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertSLA1 (SLA1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_PPE1
Plasmid#166083PurposePlasmid for constitutive spCas9 and tet-inducible PPE1 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertPPE1 (PPE1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_PDR12
Plasmid#166093PurposePlasmid for constituive spCas9 and tet-inducible PDR12 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertPDR12 (PDR12 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_TDH2
Plasmid#166082PurposePlasmid for constituive spCas9 and tet-inducible TDH2 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertTDH2 (TDH2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_LYS9
Plasmid#166085PurposePlasmid for constituive spCas9 and tet-inducible LYS9 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertLYS9 (LYS9 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSU299_GAL2
Plasmid#166088PurposePlasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeastDepositorInsertGAL2 (GAL2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterTet-inducibleAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXZ13lacZalphaKanR
Plasmid#160230PurposeLike pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJR288
Plasmid#78550PurposeFor expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
mTagRFP-T2-Tubulin-6
Plasmid#58044PurposeLocalization: Microtubules, Excitation: 555, Emission: 584DepositorInsertTubulin (TUBA1B Human)
UseTagsmTagRFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
eqFP611-H2B-6
Plasmid#56141PurposeLocalization: Nucleus/Histones, Excitation: 559, Emission: 611DepositorInsertH2B (H2BC11 Human)
UseTagseqFP611ExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
IFP1.4-H2B-C-10
Plasmid#56270PurposeLocalization: Nucleus/Histones, Excitation: 684, Emission: 708DepositorInsertH2B (H2BC11 Human)
UseTagsIFP1.4ExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRuby-Tubulin-C-18
Plasmid#55881PurposeLocalization: Microtubules, Excitation: 558, Emission: 605DepositorInsertTubulin (TUBA1B Human)
UseTagsmRubyExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits