We narrowed to 14,809 results for: Lif;

1. pET151-Vps4(101-437)

   Plasmid

   #87735

   Purpose

   Bacterial expression of N-terminally truncated S. cerevisiae Vps4

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Use

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-100

   Promoter

   T7

   Available since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-Flex-mGFP-APEX2

   Plasmid

   #171936

   Purpose

   Reporter for correlated light and electron microscopy.

   Depositor

   David Berson

   Article

   Stedehouder et al Elife. 2019 Nov 19;8. pii: 48615. doi: 1

   Insert

   mGFP-APEX2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. MCP-ADAR2-DD(E488Q, N496F)

   Plasmid

   #170139

   Purpose

   AAV vector carrying the MCP-ADAR2-DD(E488Q, N496F) with a nuclear export signal

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   NES-MCP-ADAR2-DD(E488Q, N496F)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+)-ER-CaNARi

   Plasmid

   #64852

   Purpose

   Endoplasmic reticulum (ER)-targeted FRET biosensor for monitoring calcineurin (CaN) activation; targeted to cytosolic face of ER membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CYP450-CaNARi
   

   Use

   Tags

   Cerulean, N-terminal 27 amino acids from CYP450, …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-eGFP-TAX1BP1 SKICH (1-140D)

   Plasmid

   #175768

   Purpose

   Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 SKICH (1-140D)

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   TAX1BP1 (TAX1BP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   SKICH (1-140D)

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57.1-MBD3L2

   Plasmid

   #106332

   Purpose

   All-in-one lentivector with tet-inducible MBD3L2

   Depositor

   Stephen Tapscott

   Article

   Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

   Insert

   methyl-CpG binding domain protein 3 like 2 (MBD3L2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tight TRE promoter

   Available since

   March 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJCC_058 FnCas12a D917A

   Plasmid

   #171667

   Purpose

   For bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a D917A
   

   Use

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   changed aspartate 917 to alanine

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTol1-UAS:CheRiff-tdTomato

   Plasmid

   #124234

   Purpose

   Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin CheRiff fused to the red fluorescent protein tdTomato

   Depositor

   Isaac Bianco

   Article

   Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

   Insert

   CheRiff-tdTomato
   

   Use

   Zebrafish expression

   Tags

   tdTomato

   Expression

   Mutation

   Promoter

   14xUAS-E1b

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5

   Plasmid

   #125227

   Purpose

   Expresses SPARK DRD1 transmembrane component in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5
   

   Use

   AAV

   Tags

   Flag, V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPW3781 : CMVd3-ERmembrane-GST-HaloTag-KKMP

    Plasmid

    #185686

    Purpose

    Low-level transient expression of a HaloTag protein targeted to the ER membrane, fused with a GST for constitutive dimerization.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERmembrane-GST-HaloTag-KKMP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA pDEST40 Cas9 GW STOP

    Plasmid

    #183196

    Purpose

    Destination vector

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHAGE-eGFP-OPTN S513A

    Plasmid

    #175764

    Purpose

    Lentiviral vector expressing N-terminally tagged eGFP-OPTN S513A

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S513A

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. 874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159671

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    ExuL-NLS-hSpCas9-T2A-GFP
    

    Use

    Tags

    T2A-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. FRB-CFP-ras

    Plasmid

    #87451

    Purpose

    rapamycin binding motif of mTOR (FRB) anchored to the plasma membrane

    Depositor

    Xiaodong Liu

    Article

    Liu et al Elife. 2017 Jan 6;6. pii: e21989. doi: 1

    Insert

    FRB (MTOR Human)
    

    Use

    Tags

    CFP and ras fragment

    Expression

    Mammalian

    Mutation

    FKBP and rapamycin binding domain only

    Promoter

    CMV

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Split-RESCUE-N

    Plasmid

    #170153

    Purpose

    AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    NES-MCP-RESCUE-DDN
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-CAG-DIO-mAPX

    Plasmid

    #79908

    Purpose

    Adeno-associated virus plasmid, Cre-dependent FLEX switch, mAPX

    Depositor

    Joshua Sanes

    Article

    Joesch et al Elife. 2016 Jul 7;5. pii: e15015. doi: 1

    Insert

    mAPX
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR221 Cas9-HMGB1

    Plasmid

    #183198

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-HMGB1
    

    Use

    CRISPR; Gateway entry clone

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME-APEX2-mCherry

    Plasmid

    #188944

    Purpose

    Middle entry vector for Gateway cloning containing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry

    Depositor

    Owen Tamplin

    Article

    Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

    Insert

    mito-APEX2_p2A_APEX2-H2B_p2A_mCherry
    

    Use

    Middle entry vector for gateway (l1-l2)

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221 Cas9-RFC4

    Plasmid

    #183199

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-RFC4
    

    Use

    CRISPR; Gateway entry clone

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJH2827

    Plasmid

    #179542

    Purpose

    Pnmr-1::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans AVA/E/D premotor IN and others expression of miniSOG-SL2 RFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    tomm20::miniSOG (tomm-20 Nematode)
    

    Use

    Tags

    wCherry

    Expression

    Worm

    Mutation

    Promoter

    Pnmr-1

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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