We narrowed to 21,977 results for: Sis

1. lentiCRISPR v2-sgBAX-2

   Plasmid

   #210130

   Purpose

   knock out BAX in mammalian cells

   Depositor

   Boyi Gan

   Article

   Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

   Insert

   Apoptosis regulator BAX (BAX Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-FLEX-SaCas9-U6-sgGria3

   Plasmid

   #124855

   Purpose

   Mutagenesis of Gria3

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gria3 (Gria3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-FLEXfrt-SaCas9-U6-sgGrin1

   Plasmid

   #124865

   Purpose

   Mutagenesis of Grin1

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Grin1 (Grin1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)

   Plasmid

   #100742

   Purpose

   To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis

   Depositor

   Stephen Royle

   Article

   Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

   Insert

   CD8A (CD8A Human)
   

   Use

   Tags

   LOVpep and tagRFP657

   Expression

   Mammalian

   Mutation

   Truncated

   Promoter

   CMV

   Available since

   Sept. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLVX-mCherry-KATNA1

   Plasmid

   #180650

   Purpose

   Lentiviral expression vector for KATNA1. Used for generating cell lines. Has N-terminal mCherry tag. Dox-inducible. Internal ID: WISP20-47.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   siRNA resistant to: GGACAGCACUCCCUUGAAA

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-ybbr-HIS-ELP-ddFLN4-I27-XMod-Doc (wild type)

   Plasmid

   #153442

   Purpose

   E. coli expression of Rc. XDocB wild-type construct containing ybbr tag, ELP linker and ddFLN4 and I27 fingerprint domains. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc.XDocB
   

   Use

   Tags

   3xELP linker, 6xHis, Titin I27, ddFLN4 fingerprin…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCold I-Hero13

   Plasmid

   #187929

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero13

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero13 (BEX3 Human)
   

   Use

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. hHSF1 S303A

   Plasmid

   #118363

   Purpose

   This plasmid expresses human HSF1 S303A mutant, which cannot undergo sumoylation on K298 due to disrupted PDSM motif.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hHSF1 (HSF1 Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   S303A

   Promoter

   Available since

   Nov. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZLblasti-HA-MEK1 C121S

   Plasmid

   #53165

   Purpose

   Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C121S

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

10. MSCV-TAP(FLAG-HA) CYLD

    Plasmid

    #60031

    Purpose

    CYLD with N-terminal TAP-tag (HA,FLAG) and IRES-Puro to allow tagged protein to be maintained

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

    Insert

    Cylindromatosis (CYLD Human)
    

    Use

    Retroviral

    Tags

    FLAG, HA, and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    LTR-driven expression

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

11. CD8-dCherry-FRB

    Plasmid

    #100739

    Purpose

    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis (dark mCherry K70N)

    Depositor

    Stephen Royle

    Article

    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

    Insert

    CD8A (CD8A Human)
    

    Use

    Tags

    FRB and dCherry

    Expression

    Mammalian

    Mutation

    Dark mCherry (K70N) non-fluorescent

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMMP-NGFR:LMP1-IRES-mRFP

    Plasmid

    #36974

    Depositor

    Bill Sugden

    Article

    Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

    Insert

    NGFR:LMP1 (NGFR, LMP-1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    NGFR:LMP1 chimeric receptor consists of aa 1–276 …

    Promoter

    CMV

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKIF1C-2xFLAG

    Plasmid

    #130979

    Purpose

    Expression of KIF1C-2xFLAG in mammalian cells.

    Depositor

    Anne Straube

    Article

    Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi

    Insert

    KIF1C-2xFLAG (KIF1C Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    4 silent mutations that make this construct resis…

    Promoter

    CMV

    Available since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-FLEXfrt-SaCas9-U6-sgGria3

    Plasmid

    #124869

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSUPER-retro-puro-tetO-Mek1-shRNA

    Plasmid

    #53179

    Purpose

    Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (Map2k1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pAN19-3xFLAG-TIR1-NOSt

    Plasmid

    #108545

    Purpose

    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 [Wild type] and NOS terminator

    Depositor

    Keiko Torii

    Article

    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d

    Insert

    TRANSPORT INHIBITOR RESPONSE 1 fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    

    Use

    Cloning vector

    Tags

    3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Gal4-VP16-HSF1Δ∼PRD

    Plasmid

    #71727

    Purpose

    plasmid expressing fusion construct of Gal4 BDB VP16 AD and HSF1Δ∼PRD RD

    Depositor

    Lea Sistonen

    Article

    Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

    Insert

    Gal4 DBD HSF1 Δ∼PRD RD VP16 AD (HSF1 Human, Herpes Simplex Virus protein VP16, Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-mEGFP-hCTNNA1_E307K

    Plasmid

    #234560

    Purpose

    CTNNA1 butterfly eye mutant

    Depositor

    Cara Gottardi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

    Insert

    mEGFP:hCTNNA1_E307K (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    E307K

    Promoter

    CMV

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV-mEGFP-hCTNNA1_R551A

    Plasmid

    #234558

    Purpose

    CTNNA1 M2-M3 salt-bridge disrupting mutant

    Depositor

    Cara Gottardi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

    Insert

    mEGFP:hCTNNA1_R551A (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    R551A

    Promoter

    CMV

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-mEGFP-hCTNNA1_DM2,3-domain

    Plasmid

    #234556

    Purpose

    CTNNA1 M2,3-domain deletion mutant

    Depositor

    Cara Gottardi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Aug 26:2023.08.

    Insert

    mEGFP:hCTNNA1_DM2,3-domain (CTNNA1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Deletion of aa 397-509

    Promoter

    CMV

    Available since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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