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pCDNA5/FRT/TO_H2B_HaloTag9

Plasmid

#169334

Purpose

Nuclear expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

H2B-HaloTag9 (H2BC21 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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CMV+ plasmid

Plasmid

#169740

Purpose

Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

pCMV-tetO2

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAB078d9

Plasmid

#79207

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-MKL1-~100

Plasmid

#27176

Depositor

Ron Prywes

Article

Cen et al Mol Cell Biol. 2003 Sep . 23(18):6597-60

Insert

MKL1 (MRTFA Human)

Tags

3XFLAG

Expression

Mammalian

Mutation

Deletion of 100 aa from ATG

Available Since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only
pET28_Human_SAT1_Cterm_His

Plasmid

#220487

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Nterm_His

Plasmid

#220482

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Article

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Tags

6xHs

Expression

Bacterial

Mutation

none

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_AC-CEN_ARS_HIS

Plasmid

#202129

Purpose

Level 0 plasmid containing a centromere sequence that is recognized in diatoms with AC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

CEN_ARS_HIS

Use

Synthetic Biology

Mutation

ARS sequence mutated to remove sapI site for dome…

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEY15

Plasmid

#191021

Purpose

flp-1 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

flp-1 (flp-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_TNK2-1/1

Plasmid

#91377

Purpose

Protein expression and purification of human SH3 domain construct TNK2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TNK2-1/1 (TNK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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LT3REVIN

Plasmid

#111175

Purpose

Tet-regulated miR-E (miR-30 variant)-based RNAi

Depositor

Johannes Zuber

Article

Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

Insert

miR-E (miR-30 variant)

Use

Lentiviral

Mutation

WT

Promoter

T3G

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pBABE Zeo IkB SR

Plasmid

#83023

Purpose

Retroviral expression of dominant negative IkB alpha

Depositor

Yi Zhang

Article

Kim et al Cell Rep. 2016 Sep 27;17(1):275-288. doi

Insert

NFKB Inhibitor Alpha (NFKBIA Human)

Use

Retroviral

Mutation

Serine 32 to Alanine, Serine 36 to Alanine

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pEY12

Plasmid

#191018

Purpose

Fluorescent reporter driving panneuronal nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

rab-3 (rab-3 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY46

Plasmid

#191050

Purpose

glr-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

glr-1 (glr-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pEY38

Plasmid

#191042

Purpose

unc-8 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

unc-8 (unc-8 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEY13

Plasmid

#191019

Purpose

Fluorescent reporter driving amphid and phasmid nuclear GCaMP6s expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

arrd-4 (ZK938.2 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pEY20

Plasmid

#191026

Purpose

gcy-18 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

gcy-18 (gcy-18 Nematode)

Expression

Worm

Mutation

None

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHH29_32 series plasmid

Plasmid

#120901

Purpose

Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Inserts

Ec-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pAd GFP HDAC5 WT

Plasmid

#32217

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Use

Adenoviral

Tags

GFP

Expression

Mammalian

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only
pAB085d

Plasmid

#79209

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ptet

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pL0_EF-PtBle

Plasmid

#202180

Purpose

Level 0 plasmid containing a phleomycin antibiotic cassette (fcpF promoter) with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

ShBle expression cassette

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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