We narrowed to 10,362 results for: Coli
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Plasmid#167676PurposeIntermediate vector for eight gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRAvailable SinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAJM.771
Plasmid#108534PurposeNahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.DepositorInsertNahRAM + PSalTTC-YFP
ExpressionBacterialMutationNahR: I25V P72H V118A R248CAvailable SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG034 (restriction-like)
Plasmid#157912PurposeExpresses a restriction-like defense systemDepositorInsert4-gene restriction-like system
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG026 (tmn)
Plasmid#157904PurposeExpresses the tmn defense systemDepositorInserttmn (transmembrane ATPase)
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRT801
Plasmid#68112PurposeIntegration vector derived from Streptomyces phage phiBT1. Apramycin resistance gene, oriT, ColE1 origin of replication.DepositorInsertsaac3(IV)
oriT
attP
int
UseStreptomyces integrating vectorAvailable SinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABE8r-R153del
Plasmid#208296PurposeExpresses ABE8r in mammalian cellsDepositorInsertTadA8r-R153del-SpCas9 D10A
ExpressionMammalianMutationW23R, H36L, R47K, P48A, R51L, I76Y, V82S, A106V, …Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA_MBP
Plasmid#132700Purposeexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISADepositorInsertMaltose binding Protein
TagsAvi and HisExpressionBacterialPromoterpBADAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRF0G-Tyr
Plasmid#113644PurposePlasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.DepositorInsertMjYRS and tRNA gene
UseSynthetic BiologyAvailable SinceJuly 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLG027 (qatABCD)
Plasmid#157905PurposeExpresses the qatABCD defense systemDepositorInsertqatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVS2
Plasmid#33144DepositorInsertsLacO (256 copies)
GAL-GFP-kd200-GALpA reporter
ExpressionYeastMutationGFP is mutated such that amino acids 64-73 (FSYGV…Promoterbidirectional GAL1-GAL10 promoterAvailable SinceDec. 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLG024 (SIR2 + HerA)
Plasmid#157902PurposeExpresses the SIR2 + HerA defense systemDepositorInsertSIR2 + HerA
ExpressionBacterialAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
G555
Plasmid#216483PurposeExpression of PlsB, PlsC, CdsA and PssA under the control of T7 promoters. The 4-gene insert is flanked by Phi29 replication origins.DepositorInsertplsB, plsC, cdsA, pssA
UseCell-free expressionPromoterT7 for each geneAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLG003 (Druantia type I)
Plasmid#157881PurposeExpresses the Druantia type I defense systemDepositorInsertDruantia type I
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAJM.474
Plasmid#108526PurposeLuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.DepositorInsertLuxR + PLuxB-YFP
ExpressionBacterialMutationVanR: T49I A111V P179SAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKL002
Plasmid#98921PurposepET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.DepositorInsertsPROPS
GCaMP6f
ExpressionBacterialPromoterT7Available SinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)
Plasmid#110554PurposeminiTn7 delivery plasmid with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
ExpressionBacterialPromoterAraC-ParaBADAvailable SinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHC79
Plasmid#167551PurposeBackbone plasmid to pPM1001, pPM1002, pPM1003, pPM2212 and pPM2213DepositorTypeEmpty backboneUseCosmidAvailable SinceMay 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG002 (BREX type I)
Plasmid#157880PurposeExpresses the BREX type I defense systemDepositorInsertBREX type I
ExpressionBacterialAvailable SinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMON-HsNMT1
Plasmid#67475PurposeExpression of human N-myristoyltransferase 1 for N-myristoylation of ARF family proteins in bacteriaDepositorInsertHsNMT1 (NMT1 Human)
ExpressionBacterialAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a T7pCONS sfGFP
Plasmid#154462PurposepET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available SinceAug. 14, 2020AvailabilityAcademic Institutions and Nonprofits only