Addgene

We narrowed to 2,475 results for: 683

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YCe1901 HC_Kan_PGKpA_p22

Plasmid

#100683

Purpose

part designed to occupy position 22 of EMMA. Functional category: polyadenylation signal sequence

Depositor

Yizhi Cai

Article

Martella et al ACS Synth Biol. 2017 Apr 24. doi: 10.102

Insert

PGKpA

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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TOPO CloverCP

Plasmid

#68444

Purpose

TOPO construct expressing CloverCP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

CloverCP

Use

Gmap

Tags

"CP" degradation domain

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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XE75 Beta-catenin Delta N-9 GFP

Plasmid

#16831

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

beta-catenin Delta N - 9 GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Expression

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
TOPO mT2

Plasmid

#68491

Purpose

TOPO construct expressing mT2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mTurquoise2

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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XE73 TM Beta-catenin 1-myc in CS2+

Plasmid

#16834

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

TM beta-catenin 1-myc (ctnnb1.L Frog)

Use

Xenopus expression

Tags

c-myc

Expression

Mutation

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pMS161

Plasmid

#215683

Purpose

Guide only plasmid targeting chrIII split hygromycinR landing pad

Depositor

Patrick Phillips

Article

Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

Insert

U6p::GTCCAGCGGCAGATCGGCGG

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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vYM015_phiC31-nTRE-Ins(low)-pEF1s(orig)-H2b-Citrine

Plasmid

#197683

Purpose

"Insert(low)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpM-HsPAN3_245-687-L571EG578E_U

Plasmid

#147683

Purpose

Bacterial Expression of HsPAN3_245-687-L571EG578E

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

HsPAN3_245-687-L571EG578E (PAN3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCZGY2748

Plasmid

#193331

Purpose

Site specific CRISPR/Cas9 editing of C. elegans Chr I

Depositor

Yishi Jin

Article

Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

Insert

sgRNA for ttTi4348

Use

Tags

None

Expression

Worm

Mutation

Promoter

U6

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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NK85

Plasmid

#176611

Purpose

To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

FCERG

Use

Lentiviral

Tags

EGFP and HA, SNAP-tag

Expression

Mammalian

Mutation

C25A

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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NK83

Plasmid

#176610

Purpose

To measure Syk intensity in response to ligand clustering using DNA origami

Depositor

Meghan Morrissey

Article

Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

Insert

NeonGreen-tSH2 Syk (Syk Mouse, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

n/a

Promoter

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHIS8-3_DL121

Plasmid

#171953

Purpose

Expression vector for ecDHFR-asLOV2 fusion protein. Also has TYMS

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Insert

Fusion of ecDHFR and asLOV2 at position 121 of DHFR

Use

Tags

8-His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Use

Tags

8-His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR 221-PHL2 (STOP)

Plasmid

#118683

Purpose

Gateway entry vector

Depositor

Kristiina Himanen

Article

Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

Insert

PHL2 (AT3G24120 Mustard Weed)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD-pTDH3 (01-11)

Plasmid

#101683

Purpose

pTDH3 in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

GGAG-[promoter of S. cerevisiae YGR192C (675bp)]-CCAT

Use

Yeast expression

Tags

Expression

Mutation

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode6

Plasmid

#83683

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode6

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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TOPO HRE

Plasmid

#68458

Purpose

TOPO construct expressing HRE for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Hypoxia Response Element

Use

Gmap

Tags

Expression

Bacterial

Mutation

Promoter

Plac

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pARD (pKD-G4)

Plasmid

#62683

Purpose

Mammalian expression of amiR-eGFP from the broadly active CAG promoter/enhancer

Depositor

Masato Ohtsuka

Article

Miura et al PLoS One. 2015 Aug 18;10(8):e0135919. do

Insert

amiR-eGFP419

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pFUS2_a3a

Plasmid

#50683

Purpose

Intermediate array vector

Depositor

Takashi Yamamoto

Article

Sakuma et al Sci Rep. 2013 Nov 29;3:3379. doi: 10.103

Insert

LacZ + BsaI restriction sites

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only

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Arp2/3_pBIG2abcd

Plasmid

#173683

Purpose

Insect expression of the 7-subunit Human Arp2/3 complex

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Inserts

Arp2
Arp3
ArpC2
ArpC3-FLAG
ArpC4
ArpC5
ArpC1

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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