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Plasmid#161596PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1DepositorInsertguiding RNA
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTCW018
Plasmid#83531PurposeAromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpinDepositorInsertpARO9-mf_2-pFUS1J2-ARO7i-CYC1t
UseTagsExpressionYeastMutationPromoterpARO9/pFUS1J2Available sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 IRF9
Plasmid#67079PurposeStandard for cell-free expression benchmarking.DepositorInsertIRF9 (IRF9 Human)
UseCell-free expressionTagsEGFP and HisExpressionMutationPromoterT7Available sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
PLJR965
Plasmid#115163PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosisDepositorInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR (deleted TetON) MtbCO
L5 attP
L5 Int
KanR
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNEU-hMbPylRS-4xU6M15
Plasmid#105830PurposePlasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cellsDepositorInsertsHumanized MbPylRS
tRNAM15
UseTagsExpressionMammalianMutation(Y271A, Y349F)MbPylRSPromoterCMV and U6Available sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRT029
Plasmid#127969Purposedouble-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locusDepositorInsertCLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28 His6-mEGFP-D4H
Plasmid#226373PurposeProduction of recombinant mEGFP-D4H, a fluorescent probe binding cholesterolDepositorInsertD4H (pfoA Clostridium perfringens)
UseTagsHis6 and monomeric EGFPExpressionBacterialMutationPromoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Caveolin-3
Plasmid#68396PurposeFluorescently tagged Caveolin-3 for expression in mammalian cellsDepositorInsertCav3 (Cav3 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
PLJR962
Plasmid#115162PurposeSth1 dCas9 TetR and KanR L5 Int attP for M. smegmatisDepositorInsertsSth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
EFS-EGFP-P2A-Neo-WPRE-U6-AsCas12aDR-AsCas12aDR (pRG212)
Plasmid#149722PurposeLentiviral expression of crRNA with GFP and a neomycin resistance geneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6 promoter for crRNA expression and EFS promoter…Available sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti SYN-dCas9-KRAB-MeCP2
Plasmid#155365PurposeExpresses dCas9-KRAB-MeCP2 fusion driven by human SYN promoterDepositorInsertdCas9-KRAB-MeCP2
UseCRISPR and LentiviralTags3x FLAG and 7x HisExpressionMammalianMutationPromoterSYNAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMTL3
Plasmid#127966Purposeconstitutive expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTL5
Plasmid#127967PurposeDHFR-degron controlled expression of KRAB-dCas9-BFP-KRAB from the AAVS1 locusDepositorInsertAAVS1-CAG-DHFR-KRAB-dCas9-BFP-KRAB
UseTALENTagsExpressionMammalianMutationPromoterCAGAvailable sinceNov. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
sfTq2ox-PBP5
Plasmid#117960PurposePeriplasmic expression of superfolder mTurquoise2ox (pNM077 pTHV037-DsbAss-LEGPAGL-sfTq2ox-EFGS-∆1-17-PBP5)DepositorInsertsfTq2ox-PBP5 (dacA )
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 3xFlag IFIT3
Plasmid#53553Purposemammalian expression of IFIT3DepositorInsertIFIT3 (IFIT3 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYDE009
Plasmid#194151PurposeDelivery of dcas9 into A. baumannii via Tn7 for CRISPRi. Tn7 element has tetR-tetP-dcas9, GmR; plasmid backbone is CarbR.DepositorInserttetR-tetP-dcas9-rrnB T1-T7Te
UseTagsExpressionBacterialMutationPromotertetpAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB3052(gRNA X-4, XI-3, XII-5)
Plasmid#73294PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+) 6xHis-TEV-IFIT3
Plasmid#53559Purposebacterial expression of IFIT3DepositorInsertIFIT3 (IFIT3 Human)
UseTags6xHIS and TEVExpressionBacterialMutationPromoterT7Available sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB3051(gRNA X-3 XI-2 XII-2)
Plasmid#73293PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-3, XI-2, and XII-2DepositorInsertguiding RNA
UseCRISPR; GrnaTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
DT034A
Plasmid#159395PurposeConstitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoterDepositorInsertpr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
UseSynthetic BiologyTagsFLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…ExpressionBacterialMutationPromoterJ23117 and pLux promoterAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only