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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,448 results for: NEB

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Showing: 361 - 380 of 1448 results

  4. pCMV/MASC_(GLuc)_INT

    Plasmid
    #68440
    Purpose
    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone.
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing three PP7 Stem-loops
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    68440-plasmid-map-sequence-id-124660

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  11. pU6_(GLuc)_INT[S1Apt]

    Plasmid
    #68425
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct with S1 streptavidin aptamer
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6
    Available Since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68425-plasmid-map-sequence-id-120203

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  12. pmalc4x_G252R_TEV

    Plasmid
    #47704
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation G252R
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Olfactomedin-like domain with G252R mutation (MYOC Human)
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed Glycine 252 Arginine
    Promoter
    Ptac
    Available Since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47704-plasmid-map-sequence-id-68124

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  13. pmalc4x_K398R_TEV

    Plasmid
    #47701
    Purpose
    Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation K398R
    Depositor
    Raquel Lieberman
    Article
    Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.
    Insert
    Olfactomedin-like domain with K398R mutation (MYOC Human)
    Tags
    MBP
    Expression
    Bacterial
    Mutation
    changed Lysine 398 to Arginine
    Promoter
    Ptac
    Available Since
    Sept. 9, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    47701-plasmid-map-sequence-id-68116

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  15. (SUMO)10-(SIM)5

    Plasmid
    #126866
    Purpose
    Bacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    126866-plasmid-map-sequence-id-263307

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Showing: 361 - 380 of 1448 results