We narrowed to 4,084 results for: lenti crispr plasmid

1. pL-CRISPR.EFS.tRFP

   Plasmid

   #57819

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses tagRFP via P2A cleavage site. EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-tRFP
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL-CRISPR.EFS.PAC

   Plasmid

   #57828

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, EFS Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2

   Plasmid

   #216173

   Purpose

   Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

   Depositor

   Makiko Iwafuchi

   Article

   Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

   Insert

   sgRNA- FOXA.bs- ZEB2.locus
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Lenti-TargetBC-5xTAPE-1-pegRNA-InsertBC

   Plasmid

   #183790

   Purpose

   A single construct out of the pool of plasmid for lineage recording experiment using DNA Ticker Tape.

   Depositor

   Jay Shendure

   Article

   Choi et al Nature. 2022 Jul 6. pii: 10.1038/s41586-

   Inserts

   P2A-eGFP-TargetBC-5xTAPE-1
   pegRNA-InsertBC
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   G>A in the gRNA scaffold- please see depositor…

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-sgRNA-Cre-GpNLuc

   Plasmid

   #208382

   Purpose

   Derived from LentiCRISPRV2 by replacing Cas9 with Cre recombinase and puromycin resistance with GpNLuc. Contains a stuffer sequence for cloning of sgRNA of interest, as per LentiCRISPRV2.

   Depositor

   Gaorav Gupta

   Article

   Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

   Type

   Empty backbone

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LentiGuide-Neo-NCOA7g1 (BB34)

   Plasmid

   #139452

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-Neo-NCOA7g3 (BB36)

   Plasmid

   #139454

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LentiGuide-Neo-NCOA7g2 (BB35)

   Plasmid

   #139453

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: neoR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. L40C-CRISPR.EFS.mNeon

   Plasmid

   #69146

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter driven

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Inserts

   SpCas9
   Sp sgRNA scaffold
   EFS (EEF1A1 Human)
   P2A-mNeonGreen
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

    Plasmid

    #207180

    Purpose

    Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-SFFVp-CRISPRoffv2.1-IRES-BlastR

    Plasmid

    #207181

    Purpose

    Lentiviral Expression Plasmid for CRISPRoff2.1 driven by SFFV Promoter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFVp

    Available since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-EGFR-L858R-T790M-dual-nick sgRNA

    Plasmid

    #214101

    Purpose

    Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.

    Depositor

    Ting Zhou

    Article

    Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

    Insert

    EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    June 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. L-CRISPR-CTN (MLL-ENL)

    Plasmid

    #69212

    Purpose

    Lentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Inserts

    SpCas9
    Sp sgRNA scaffold
    EFS (EEF1A1 Human)
    P2A-mNeonGreen
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd

    Plasmid

    #216170

    Purpose

    Express the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- FOXA.bs- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FgH1tUTG-sgRNA.Control-ZEB2_CRISPRd

    Plasmid

    #216172

    Purpose

    Express the gRNA targeting the control site in the human ZEB2 locus for the CRISPRd experiment

    Depositor

    Makiko Iwafuchi

    Article

    Matsui et al Mol Cell. 2024 Jan 4:S1097-2765(23)01026

    Insert

    sgRNA- Control- ZEB2.locus
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Lenti-RNF2-ngRNA+41_EF1a-puroR

    Plasmid

    #207358

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 +41 ngRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Lenti-sgRNA-Cre-GpNluc-sgControl

    Plasmid

    #208383

    Purpose

    This sgControl, located in the TP53BP1 intron, serves as a control sgRNA for the others. The vector was cloned from Lenti-sgRNA-Cre-GpNLuc.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgControl (TP53BP1 intron) (Trp53bp1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#1

    Plasmid

    #208387

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#1 gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgZBP1 (Zbp1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-sgRNA-Cre-GpNLuc-sgmZBP1#2

    Plasmid

    #208388

    Purpose

    Lentiviral vector expressing Cas9, GpNLuc, and sgRNA targeting murine ZBP1#2 gene.

    Depositor

    Gaorav Gupta

    Article

    Cho et al Nature. 2024 Jan 10. doi: 10.1038/s41586

    Insert

    sgZBP1 (Zbp1 Mouse)
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Jan. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-N1303K-ngRNA+14_EF1a-puroR

    Plasmid

    #207359

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    N1303K-CFTR +14 ngRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge