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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 813 results for: neb

Showing: 361 - 380 of 813 results

  1. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q-A60C

    Plasmid
    #114618
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 37 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, A60C; 37 polyQ repeat
    Promoter
    Available Since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    114618-plasmid-map-sequence-id-222655

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  2. pTWIN1-His6-Ssp-Htt2-90(A2C)-15Q-A60C

    Plasmid
    #114608
    Purpose
    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & A60C cysteine mutations and a 15 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Use
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    A2C, A60C; 15 polyQ repeat
    Promoter
    Available Since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    114608-plasmid-map-sequence-id-222653

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  4. pUC19-Q03JI6

    Plasmid
    #103123
    Purpose
    Cas9 coding gene template with optimized sequence for human codon usage
    Depositor
    Duhee Bang
    Article
    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55
    Insert
    Q03JI6 (Cas9 coding gene from Campylobacter jejuni)
    Use
    CRISPR; Cloning vector
    Tags
    SV40NLS
    Expression
    Mutation
    human codon-optimized
    Promoter
    Available Since
    April 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    103123-plasmid-map-sequence-id-201885

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  12. PaPchF-AMT

    Plasmid
    #242104
    Purpose
    Adenylase-methyltransferase didomain of the nonribosomal peptide synthetase Pchf from Pseudomonas aeruginosa for the production of pyochelin
    Depositor
    Audrey Lamb
    Article
    Ronnebaum et al Biochemistry. 2019 Feb 12;58(6):665-678.
    Insert
    PaPchF-AMT
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Sept. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    242104-plasmid-map-sequence-id-486672

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  15. pJYS1Peftu

    Plasmid
    #85546
    Purpose
    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter
    Depositor
    Sheng Yang
    Article
    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.
    Inserts
    Cpf1
    recT
    Use
    CRISPR; Shuttle vector corynebacterium glutamicum…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    etfu and unknown
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85546-plasmid-map-sequence-id-176968

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  18. pJYS1Ptac

    Plasmid
    #85545
    Purpose
    Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter
    Depositor
    Sheng Yang
    Article
    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.
    Inserts
    Cpf1
    recT
    Use
    CRISPR; Shuttle vector corynebacterium glutamicum…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    tac and unknown
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    85545-plasmid-map-sequence-id-176966

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  19. pCgCas9_recT

    Plasmid
    #153337
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; Kanr
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    Cas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153337-plasmid-map-sequence-id-305977

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  20. pCgsgRNA_crtYf

    Plasmid
    #153338
    Purpose
    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr
    Depositor
    Sheng Yang
    Article
    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19
    Insert
    crRNA targeting crtYf
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    153338-plasmid-map-sequence-id-306018

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Showing: 361 - 380 of 813 results