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Plasmid#180794PurposeExpression of human PD-1 extracellular domain and transmembrane domain fused to BTLA intracellular domain followed by mEGFPDepositorUseLentiviralTagsmEGFPExpressionMutationPromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only
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pHR-SV40-BTLAecto&tmd-PD-1icd-mGFP
Plasmid#180795PurposeExpression of human BTLA extracellular domain and transmembrane domain fused to PD-1 intracellular domain followed by mEGFPDepositorUseLentiviralTagsmEGFPExpressionMutationPromoterSV40Available sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY232
Plasmid#184149Purposeshort tracrRNA generator with WT anti-repeat regionDepositorInserts-tracrRNA-WT
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD63-(TEV)-tTA
Plasmid#162597Purposehuman CD63-TEV cleavage site-tTADepositorInserthuman CD63 (CD63 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-Cre
Plasmid#167939Purposehuman CD81-TEV cleavage site-CreDepositorInserthuman CD81 (CD81 Human)
UseTagsCre recombinase and TEV protease cleavage siteExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCOM-CD63-COM
Plasmid#138350PurposeExpressing COM-CD63-COM fusion protein in mammalian cellsDepositorInsertCOM-CD63-COM fusion protein
UseCRISPR and LentiviralTagsCOM is fused to the N and C termini of human CD63ExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBC114
Plasmid#202276PurposeLevel 2 for strain-specific barcode DNA tag with bc141 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc141
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD81-(TEV)-tTA
Plasmid#162598Purposehuman CD81-TEV cleavage site-tTADepositorInserthuman CD81 (CD81 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAOJ15
Plasmid#91567PurposeBroad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoterDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterTetracycline promoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CD9-(TEV)-tTA
Plasmid#162596Purposehuman CD9-TEV cleavage site-tTADepositorInserthuman CD9 (CD9 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV/N3IC-HA
Plasmid#47618Purposein vitro transcription and translation of HA-tagged Notch3 Intracellular domainDepositorInsertNotch3 Intracellular domain (Notch3 Mouse)
UseIn vitro transcription and translationTagsHAExpressionMammalianMutationcodons 1664 (Met) to 2318 (Ala)PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLY239
Plasmid#184152PurposeReprogrammed short tracrRNA generator for hijacking RFP mRNA as CRISPR RNA (target site 3)DepositorInserts-tracrRNA-R3
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLY273
Plasmid#184155PurposeReprogrammed short tracrRNA for hijacking mRNA of zraPDepositorInserts-tracrRNA-zraP-22
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBC113
Plasmid#202275PurposeLevel 2 for strain-specific barcode DNA tag with bc140 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc140
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC115
Plasmid#202277PurposeLevel 2 for strain-specific barcode DNA tag with bc142 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlumDepositorInsertbc142
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC117
Plasmid#202278PurposeLevel 2 for strain-specific barcode DNA tag with bc144 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent markerDepositorInsertbc144
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC118
Plasmid#202279PurposeLevel 2 for strain-specific barcode DNA tag with bc145 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent markerDepositorInsertbc145
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBC119
Plasmid#202280PurposeLevel 2 for strain-specific barcode DNA tag with bc146 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent markerDepositorInsertbc146
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBCC092
Plasmid#202219PurposeLevel 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and AmCyan1 fluorescent markerDepositorInsertpBCC25, pBCC30, pBCC56, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBCC093
Plasmid#202220PurposeLevel 2 empty backbone for strain-specific barcode DNA tag through Tn7 bacterial insertion, Tetracyline selectable marker and tagRFP fluorescent markerDepositorInsertpBCC25, pBCC30, pBCC58, pBCC27, pBCC26, pBCC28, pICH41822 (G5)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only