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pCEP4-myc-HLA-A2-VN (Y84P)

Plasmid

#135495

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (Y84P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

Y84P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A2-VN (E166P)

Plasmid

#135498

Purpose

Mammalian expression of VN-fused and myc-tagged HLA-A*02:01 (E166P mutant)

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*02:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag, Signal peptide f…

Expression

Mammalian

Mutation

E166P

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-AuroraB L154A/H250Y

Plasmid

#108491

Purpose

expression of EGFP-AuroraB Analog sensitive (L154A/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

EGFP

Expression

Mammalian

Mutation

Analog sensitive L154A/H250Y

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-INCENP d543-746

Plasmid

#108473

Purpose

expression of vsv-INCENP d543-746

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

INCENP d543-746 AA (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

Delta Alpha helix, aa543-746 deleted

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shGBP1.2.mKO2

Plasmid

#85210

Purpose

TRCN0000116120 (Target TGAGACGACGAAAGGCATGTA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.

Depositor

Sandra Martha Gomes Dias

Article

Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

Insert

GBP1 (guanylate binding protein 1) (GBP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

RNA polymerase III promoter for human U6 snRNA fo…

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a I18A

Plasmid

#40043

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a I18A (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Isoleucine 18 to Alanine

Promoter

CMV promoter

Available Since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a linker

Plasmid

#40040

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a linker (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

linker domain (144-353)

Promoter

cmv

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only
5` CC: Puro – loxP – GFP-C

Plasmid

#219563

Purpose

5` circularization cassette.Used in combination with one of the 3' circularization cassettes to induce Cre-mediated circularizazion or inversion of a desired genomic region.

Depositor

Andrea Ventura

Article

Pradella et al Nature. 2024 Dec 18. doi: 10.1038/s41586

Insert

hPGK-promoter_PuroR_2A_loxP_GFP-C

Use

Unspecified

Promoter

hPGK

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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CIRTS-control

Plasmid

#213788

Purpose

CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.

Depositor

Timothy Bredy

Article

Liau et al Nat Commun. 2023 Nov 22;14(1):7616. doi:

Insert

CIRTS-Calm3-U6-control gRNA

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

Syn1, U6

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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p5E-EFS (EF1a core) (JDW 1319)

Plasmid

#224474

Purpose

Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

EFS promoter

Expression

Mammalian

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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RUNX1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176974

Purpose

Mammalian expression vector encoding RUNX1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

RUNX1 (RUNX1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-BUBR1

Plasmid

#234704

Purpose

expression of fusion protein

Depositor

Lienhard Schmitz

Article

Seibert et al J Cell Biol. 2019 Apr 1;218(4):1164-1181

Insert

BUBR1 (BUB1B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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9_T7-mScarlet3-LactC2

Plasmid

#225936

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al Development. 2026 Apr 15;153(8):dev20541

Insert

mScarlet3-LactC2

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-DEST (JDW 1186)

Plasmid

#229820

Purpose

An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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Cre-IRES-PuroR

Plasmid

#30205

Purpose

Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

Depositor

Darrell Kotton

Article

Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

Insert

Cre recombinase (cre Enterobacteria phage P1)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

EF1alpha

Available Since

April 12, 2012

Availability

Academic Institutions and Nonprofits only

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GLI1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176968

Purpose

Mammalian expression vector encoding GLI1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

GLI1 (GLI1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry-N1 GBP (GBP-mCherry)

Plasmid

#162879

Purpose

Expression in mammalian cells of GFP binding protein (GBP) tagged with mCherry

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

GFP Binding Protein

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn1_HyPer7_mito

Plasmid

#238968

Purpose

Mammalian neurons HyPer7 expression targeted to the mitochondrial matrix

Depositor

Dmitry Bilan

Article

Kelmanson et al Redox Biol. 2021 Nov 3;48:102178. doi: 1

Insert

HyPer7

Use

AAV

Tags

3x Cytochrome C Oxidase Subunit VIII presequense

Expression

Mammalian

Promoter

hSyn1

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pME Actin Vhh Halo Tag (JDW 1223)

Plasmid

#224498

Purpose

Gateway compatible middle entry clone containing an Actin nanonbody fused to a flexible linker and Halo Tag (For visualizing actin, actin chromobody)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Actin-Vhh-Halo-Tag

Use

Gateway cloning

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1-E-'No'Mi-Red-T2A-Puro

Plasmid

#246642

Purpose

EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.

Depositor

Koen Breyne

Article

Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70

Insert

cleavable E-NoMi-Red

Use

Lentiviral

Tags

FLAG-Nanoluciferase and mCherry

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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