We narrowed to 42,360 results for: Tro;

1. pLVPT-rtTR-KRAB

   Plasmid

   #11777

   Purpose

   Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

   Depositor

   Patrick Aebischer, Didier Trono

   Article

   Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

   Insert

   mPGK, GFP, rtTR-KRAB, Tet-off
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 17, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCW02

   Plasmid

   #72301

   Purpose

   Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Type

   Empty backbone

   Use

   Derivative of puc19

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Aug. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLys-M1

   Plasmid

   #109382

   Purpose

   Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

   Depositor

   Tom Ellis

   Article

   Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

   Insert

   pARABAD-VioB-mCherry
   

   Expression

   Bacterial

   Available Since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSP64-membrane-mCherry-LOV

   Plasmid

   #135599

   Purpose

   Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus

   Depositor

   Mamiko Yajima

   Article

   Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

   Insert

   membrane-mCherry-LOV
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   LOV and mCherry

   Promoter

   Unknown

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICH51288

   Plasmid

   #50269

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL2_166

   Plasmid

   #199098

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Nili Ostrov, Cultivarium Tools, Charlie Gilbert

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   RK2 ORI plus NGS barcode 12, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-pDisplay-Halo

   Plasmid

   #223497

   Purpose

   Expression of HaloTags to outer surface in mammalian cells

   Depositor

   James Frank

   Article

   Tobias et al Chem Sci. 2021 Sep 15;12(40):13506-13512

   Insert

   HaloTag
   

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAC-EsaR

   Plasmid

   #47643

   Purpose

   wt esaR gene under control of Plac in pACYC184

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   Plac-esaR-terminator
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pGL2_167

   Plasmid

   #199099

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Nili Ostrov, Cultivarium Tools, Charlie Gilbert

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGL2_170

    Plasmid

    #199102

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Nili Ostrov, Cultivarium Tools, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pSa ORI plus NGS barcode 16, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECFP-DEVG-Venus

    Plasmid

    #24538

    Depositor

    Peter Sorger

    Article

    Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

    Insert

    ECFP-DEVG-Venus
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    DEVDR mutated to DEVG

    Promoter

    CMV

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pHB-2

    Plasmid

    #140955

    Purpose

    For PHB production in E. coli (T7-pAC mutant). Please note that this is listed as #140954 in the publication.

    Depositor

    Kang Zhou

    Article

    Liu et al Metab Eng. 2020 Sep;61:206-214. doi: 10.

    Insert

    phaCAB
    

    Expression

    Bacterial

    Mutation

    R238S mutation in PhaC

    Available Since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZac2.1 hSynapsin1 FLEX NAPA-N SV40

    Plasmid

    #92283

    Purpose

    FRET probe; extracellular synapse targeted mCherry, Cre-dependently expressed

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Neuronal
    

    Use

    AAV

    Promoter

    hSynapsin1

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tol2-krt4-RFP

    Plasmid

    #97149

    Purpose

    As a control of miRNA sponge

    Depositor

    Qing Deng

    Article

    Zhou et al Cell Rep. 2018 Feb 13;22(7):1810-1823. d

    Type

    Empty backbone

    Use

    Zebrafish

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-cdc2 (716)

    Plasmid

    #61840

    Purpose

    mammalian expression of cdc2

    Depositor

    Jonathon Pines

    Article

    Hagting et al EMBO J. 1998 Jul 15;17(14):4127-38.

    Insert

    cdc2 (CDK1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

16. SAFB1-pcDNA3.1

    Plasmid

    #32742

    Depositor

    Steffi Oesterreich

    Article

    Townson et al J Biol Chem. 2003 May 30;278(22):20059-6

    Insert

    SAFB1 (SAFB Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pMVS1111A:PhmtB-bgaB

    Plasmid

    #205998

    Purpose

    Conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus encoding beta-galactosidase

    Depositor

    Bastian Molitor

    Article

    Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

    Insert

    thermostable beta-galactosidase
    

    Promoter

    PhmtB

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRc/CMV HA TrkA

    Plasmid

    #39977

    Depositor

    Yves-Alain Barde

    Article

    Bibel et al EMBO J. 1999 Feb 1;18(3):616-22.

    Insert

    TrkA (Ntrk1 Rat)
    

    Tags

    BM40 signal sequence and HA

    Expression

    Mammalian

    Mutation

    endogenous signal sequence was replaced with BM40…

    Promoter

    CMV

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSETB-de4GFP

    Plasmid

    #85472

    Purpose

    Inducible expression of dual emitting pH sensitive GFP in bacterial cells

    Depositor

    S. James Remington

    Article

    Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

    Insert

    de4GFP
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S65T/C48S/H148C/T203C

    Promoter

    T7

    Available Since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1455_pDEST_miniTol2_R4-R2_MCS

    Plasmid

    #171794

    Purpose

    pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a Multiple Cloning Site for selection marker insertion.

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Type

    Empty backbone

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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