We narrowed to 48,845 results for: des.1

1. pUb FLAG-IntS6 AA 1-600

   Plasmid

   #195072

   Purpose

   Expresses FLAG-tagged Drosophila IntS6 amino acids 1-600

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   IntS6 (IntS6 Fly)
   

   Use

   Tags

   3xFLAG

   Expression

   Insect

   Mutation

   Promoter

   Ubi-p63e

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-THTAP-VPS9D1, residues 1-97

   Plasmid

   #180640

   Purpose

   Bacterial expression for VPS9D1 MIT domain, residues 1-97. Has N-terminal HIS-GST tag. Internal ID: WISP20-89.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   VPS9D1 (C16orf7 Human)
   

   Use

   Tags

   HIS-GST

   Expression

   Bacterial

   Mutation

   Residues 1-97

   Promoter

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCA528 KATNA1 MIT residues 1-79

   Plasmid

   #180614

   Purpose

   Bacterial expression for KATNA1 MIT domains, residues 1-79. Has N-terminal HIS-SUMO tag. Internal ID: WISP19-20.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNA1 (KATNA1 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-79

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCA528 KATNAL1 MIT residues 1-87

   Plasmid

   #180618

   Purpose

   Bacterial expression for KATNAL1 MIT domains, residues 1-87. Has N-terminal HIS-SUMO tag. Internal ID: WISP20-54.

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   KATNAL1 (KATNAL1 Human)
   

   Use

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 1-87

   Promoter

   T7

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#1

   Plasmid

   #197421

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #1 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT7-EGFP-C1-HsNXF-1-R233AR276A_O

   Plasmid

   #147120

   Purpose

   Mammalian Expression of HsNxf-1-R233AR276A

   Depositor

   Elisa Izaurralde

   Article

   Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

   Insert

   HsNxf-1-R233AR276A (NXF1 See Depositor Comments Below, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. RPS15A sgRNA-1 lentiCRISPR v2 plasmid

   Plasmid

   #192227

   Purpose

   lentiviral vector expressing sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR

   Depositor

   Dieter Wolf

   Article

   Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

   Insert

   sgRNA-1 targeting eIF3 binding site of RPS15A 5'UTR (RPS15A Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-PD-1 (BTLA ITIM)-mGFP

   Plasmid

   #180817

   Purpose

   Expression of human PD-1 with immunoreceptor tyrosine-based inhibitory motif (ITIM) from BTLA, fused to mEGFP

   Depositor

   Enfu Hui

   Article

   Xu et al Elife. 2021 Nov 4;10. pii: 74276. doi: 1

   Insert

   PD-1 (BTLA ITIM) (PDCD1 Human)
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mutation

   replaced PD-1-ITIM (VDYGEL) with BTLA-ITIM (IVYAS…

   Promoter

   SFFV

   Available since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3/flag-pBirA(1-K283)-FRB

   Plasmid

   #154451

   Purpose

   expresses flag-pBirA(1-K283)-FRB/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pBirA(1-K283)-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCLXSN(GFP)-HA-Tbkbp1 1-280

    Plasmid

    #125178

    Purpose

    Retroviral expression of mouse Tbkbp1 (1-280)

    Depositor

    Shao-Cong Sun

    Article

    Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

    Insert

    Tbkbp1 (Tbkbp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-280

    Promoter

    CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLXSN(GFP)-HA-Tbkbp1 1-330

    Plasmid

    #125179

    Purpose

    Retroviral expression of mouse Tbkbp1 (1-330)

    Depositor

    Shao-Cong Sun

    Article

    Zhu et al Nat Commun. 2018 Jul 18;9(1):2812. doi:

    Insert

    Tbkbp1 (Tbkbp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-330

    Promoter

    CMV

    Available since

    May 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. TET-pLKO.1 PURO shGDF11 #2

    Plasmid

    #83084

    Purpose

    Lentiviral shRNA vector for inducible knockdown of human GDF11

    Depositor

    Kevin Janes

    Article

    Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

    Insert

    shGDF11 (GDF11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

13. LSB-hsa-miR-218-1-3p

    Plasmid

    #103356

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa_miR-218-1-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa_miR-218-1-3p target
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPCX2-HA-GATA3 N1(1-259)

    Plasmid

    #89830

    Purpose

    Express GATA3 N1 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    N1 (AA 1-259)

    Promoter

    CMV

    Available since

    May 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLPCX2-HA-GATA3 N2(1-294)

    Plasmid

    #89831

    Purpose

    Express GATA3 N2 in mammalian cells

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Gata3 (Gata3 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    N2 (AA 1-294)

    Promoter

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETDUET1-hUCH37 (ISF1) - hNFRKB (1-117)

    Plasmid

    #61941

    Purpose

    Co-expression of hUCH37 (ISF1) and hNFRKB (1-117) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Inserts

    hUCH37 (ISF1) (UCHL5 Human)
    hNFRKB (1-117) (NFRKB Human)
    

    Use

    Tags

    6x HIS

    Expression

    Bacterial

    Mutation

    Construct ends at residue 117, stop codon introdu…

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

17. Kv7.4 (1-645, Δ368-492)/pcDNA3.1

    Plasmid

    #111454

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-492), C643A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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18. PGC-1 alpha promoter 2kb luciferase

    Plasmid

    #8887

    Depositor

    Bruce Spiegelman

    Article

    Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

    Insert

    PGC-1 alpha promoter (Ppargc1a Mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CHOP promoter (-649/+136) pmCherry-1

    Plasmid

    #36035

    Depositor

    Quan Lu

    Article

    Oh et al J Biol Chem. 2012 Feb 17;287(8):6025-34.

    Insert

    CHOP promoter (-647 to +136) (DDIT3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMVp-dCas9-3xNLS-VP64 (Construct 1)

    Plasmid

    #55195

    Purpose

    Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/hUBC

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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