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pLV-Puro-CMV-EPB41L4A-AS1-unspliced MUT

Plasmid

#242750

Purpose

Expresses unspliced EPB41L4A-AS1 in mammalian cells

Depositor

Igor Ulitsky

Article

Monziani et al bioRxiv 2025.04.08.647803

Insert

EPB41L4A-AS1 (EPB41L4A-AS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

AACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…

Promoter

CMV

Available Since

Sept. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-HSF1-VIRF2

Plasmid

#218557

Purpose

Encodes SFFV promoter-driven MCP-HSF1-VIRF2 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-HSF1-VIRF2

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-MLLx3-HSF1

Plasmid

#218558

Purpose

Encodes SFFV promoter-driven MCP-MLLx3-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-MLLx3-HSF1

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pLex307-UCOE-SFFV-puroR-MCP-P65-HSF1

Plasmid

#218559

Purpose

Encodes SFFV promoter-driven MCP-P65-HSF1 with an N-terminal bipartite SV40 NLS, along with puromycin resistance

Depositor

Alejandro Chavez

Article

Giddins et al Nat Commun. 2025 Nov 20;16(1):11114. doi

Insert

MCP-P65-HSF1

Use

Lentiviral

Tags

SV40NLS

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Available Since

Aug. 13, 2025

Availability

Academic Institutions and Nonprofits only

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LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

Plasmid

#233442

Purpose

PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGGS puro -GFP-delta73-82 REX1-IRES-mCherry

Plasmid

#232246

Purpose

Mammalian expression of delta73-82 REX1-GFP-IRES-mCherry

Depositor

Francisco Bustos

Article

Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

Insert

delta73-82 REX1-GFP-IRES-mCherry (Zfp42 Mouse)

Tags

AcGFP1

Expression

Mammalian

Mutation

delta73-82

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Neuroligin-3 [N110/29R-rat IgG2a] - Chimeric

Plasmid

#231821

Purpose

Mammalian expression plasmid of anti-Neuroligin-3 (Rat) rat IgG2a R-mab. Derived from hybridoma N110/29.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Neuroligin-3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Nlgn3 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

Dual CMV

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0230 EF1a_Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry_Gen2

Plasmid

#231207

Purpose

Single transcript cageIL-10 (mRens) + ER-renin (H2M) (Lenti)

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Puro_ERret-H2M-Renin_T2A_mRens-cageIL-10-c-myc_T2A-mCherry (IL10 Human)

Use

Lentiviral

Mutation

L147I, R148H, I203V, L290V

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-puro SNAP-CD86-eGFP-FKBP(f36v)

Plasmid

#200283

Purpose

Membrane protein fusion with extracellular SNAPtag and intracellular FKBP(f36v) dimerization domain and GFP reporter under Tetracycline-inducible promoter

Depositor

Kristina Ganzinger

Article

Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

Insert

SNAPtag-eGFP-CD86tm-eGFP-FKBP(f36v)

Use

Lentiviral

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-p120catenin(Canis)-exon4 116-138 gRNA

Plasmid

#209923

Purpose

A knock-out vector for the dog CTNND1

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

A gRNA targeting the dog CTNND1 gene.

Use

CRISPR and Lentiviral

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Squash-FTL-IRE

Plasmid

#216828

Purpose

Expresses a circular RNA containing a Squash aptamer and an IRE (FTL derived) in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Squash-FTL-IRE

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAV-puro-U6+27-F30-Tornado-Squash-TfR-IRE

Plasmid

#216829

Purpose

Expresses a circular RNA containing a Squash aptamer and an IRE (TfR derived) in mammalian cells, using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Hagen et al Cell Chem Biol. 2024 Aug 23:S2451-9456(2

Insert

Tornado-Squash-TfR-IRE

Expression

Mammalian

Promoter

U6+27

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Plasmid

#217970

Purpose

Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

Depositor

Robert Piper

Article

Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

Insert

2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-IRES-puro6 gal4DBD-HIFCAD myc tag

Plasmid

#207171

Purpose

expression plasmid for Gal4DBD-HIF-CAD.myc expression

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD Myc tag linker

Tags

2xMyc tag

Expression

Mammalian

Promoter

EF1a

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP

Plasmid

#211343

Purpose

Expression of codon-optimized mitochondrial base editor in human cells; cell sorting; puromycin drug selection

Depositor

Monkol Lek

Insert

m.3075-Right Puro DdCBE-G1397-C- T1413I-GFP

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)

Plasmid

#214882

Purpose

Lentiviral vector encoding RfxCas13d targeting PROX guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crPROX-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-NPY2R/Neuropeptide Y receptor type 2 [L119/134R]

Plasmid

#188173

Purpose

Mammalian Expression Plasmid of anti-NPY2R/Neuropeptide Y receptor type 2 (Human). Derived from hybridoma L119/134

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-NPY2R/Neuropeptide Y receptor type 2 (Homo sapiens) recombinant mouse monoclonal antibody (NPY2R Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 T52A (B)-C-Myc-DDK-P2A-Puro

Plasmid

#211621

Purpose

Rhno1 T52A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

T52A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S51A (A)-C-Myc-DDK-P2A-Puro

Plasmid

#211620

Purpose

Rhno1 S51A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S51A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-h.Rhno1 S163A (E)-C-Myc-DDK-P2A-Puro

Plasmid

#211624

Purpose

Rhno1 S163A

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

hRHNO1 (RHNO1 Human)

Tags

myc-flag

Expression

Mammalian

Mutation

S163A

Promoter

CMV

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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