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Plasmid#105494PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible BitlucOptDepositorInsertBitlucopt
UseAtc-dependent expression in c. difficileTagsExpressionMutationPromoterPtetAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG007 (Ec86 retron)
Plasmid#157885PurposeExpresses the Ec86 retron defense systemDepositorInsertEc86 retron
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3xFLAG-V5-ccdB
Plasmid#87064PurposeMammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneUseTags3xFLAG-V5ExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMH-HA
Plasmid#101767PurposeGateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion proteinDepositorTypeEmpty backboneUseTagsHAExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLP1
Plasmid#209988PurposeEntry vector to store Level 0 partsDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA24N_6xHis-TEV-rpsA
Plasmid#83585PurposeExpress E. coli ribosomal protein S1 with a TEV-cleavable, N-terminal His tagDepositorInsertrpsA (rpsA Escherichia coli)
UseTagsHis tagExpressionBacterialMutationPromoterp-T5 LacAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQCXP CMV/TO DEST (w360-1)
Plasmid#17386PurposeRetroviral Gateway destination vector, Expression, CMV/TO promoter, PuroDepositorTypeEmpty backboneUseRetroviral; Destination vectorTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTECH-chAcK3RS(IPYE)
Plasmid#104069PurposeExpression plasmid encoding chAcK3RS(IPYE) and pylTDepositorInsertPlpp chAcK3RS(IPYE); PproK pyl
UseTagsExpressionBacterialMutationContains activating mutations V31I, T56P, H62Y, A…PromoterPlpp and PproKAvailable sinceDec. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Dest-pA-pgk-bsd
Plasmid#74918PurposeGateway destination piggyBac vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBA630
Plasmid#186241PurposeRfxCas13d Negative Control (RFP targeting)DepositorInsertpTet-RfxCas13d + crRNA negative control
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG025 (DUF4297 + HerA)
Plasmid#157903PurposeExpresses the DUF4297 + HerA defense systemDepositorInsertDUF4297 + HerA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETAipi-ispA/dxs
Plasmid#178302PurposeProduces beta-carotene in E. coli; pAC-BETAipi inserted with an operon containing ispA and dxs from E. coli.DepositorInsertsispA
dxs
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLG013 (DRT type 5)
Plasmid#157891PurposeExpresses the DRT type 5 defense systemDepositorInsertDRT type 5
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDSW1728
Plasmid#120812PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)DepositorInsertred fluorescent protein (mCherry), codon-optimized for C. difficile
UseTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB129
Plasmid#68583PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagstdTomatoExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLG027 (qatABCD)
Plasmid#157905PurposeExpresses the qatABCD defense systemDepositorInsertqatABCD (ATPase + unknown protein + QueC homolog + TatD DNase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-MYC
Plasmid#101765PurposeGateway-compatible destination vector for mammalian expression of N-terminal Myc-tag fusion proteinDepositorTypeEmpty backboneUseTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLG003 (Druantia type I)
Plasmid#157881PurposeExpresses the Druantia type I defense systemDepositorInsertDruantia type I
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG023 (dsr2)
Plasmid#157901PurposeExpresses the dsr2 defense systemDepositorInsertdsr2 (SIR2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only